|Positive WB detected in||HEK-293 cells, Purified protein, HepG2 cells, C6 cells, RAW 264.7 cells|
|Positive IP detected in||mouse spleen tissue|
|Positive IHC detected in||human breast cancer tissue, human liver cancer tissue, human tonsillitis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||A549 cells|
|Western Blot (WB)||WB : 1:1000-1:6000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
The immunogen of 17373-1-AP is PADI4 Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||PADI4 fusion protein Ag11068|
|Full Name||peptidyl arginine deiminase, type IV|
|Calculated molecular weight||663 aa, 74 kDa|
|Observed molecular weight||67 kDa|
|GenBank accession number||BC025718|
|Gene ID (NCBI)||23569|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
PADI4, also named as PADI5 and PDI5, catalyzes the citrullination/deimination of arginine residues of proteins such as histones, thereby playing a key role in histone code and regulation of stem cell maintenance. PADI4 is expressed in hematologic and rheumatoid arthritis synovial tissues. PADI4 haplotype associated with susceptibility to rheumatoid arthritis increases production of citrullinated peptides, leading, in a cytokine-rich milieu, to a break in tolerance to citrullinated peptides processed and presented in the appropriate HLA context. Immunohistochemistry detected significant PADI4 expression in various malignancies and PADI4 expression was not detected in non-tumor tissues.(PMID: 19183436)
|Product Specific Protocols|
|WB protocol for PADI4 antibody 17373-1-AP||Download protocol|
|IHC protocol for PADI4 antibody 17373-1-AP||Download protocol|
|IF protocol for PADI4 antibody 17373-1-AP||Download protocol|
|IP protocol for PADI4 antibody 17373-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Thioredoxin Modulates Protein Arginine Deiminase 4 (PAD4)-Catalyzed Citrullination.
Cold-inducible RNA-binding Protein Induces Neutrophil Extracellular Traps in the Lungs during Sepsis.
Invest Ophthalmol Vis Sci
Murine Retinal Citrullination Declines With Age and is Mainly Dependent on Peptidyl Arginine Deiminase 4 (PAD4).
Innate and Autoimmunity in the Pathogenesis of Inherited Retinal Dystrophy.
IL-6-PAD4 axis in the earliest phase of arthritis in knock-in gp130F759 mice, a model for rheumatoid arthritis.
BMC Musculoskelet Disord
Decreased severity of experimental autoimmune arthritis in peptidylarginine deiminase type 4 knockout mice.