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  • KD/KO Validated

PARD3 Polyclonal antibody

PARD3 Polyclonal Antibody for WB, IP, IF, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse and More (2)





Cat no : 11085-1-AP


ASIP, Baz, Bazooka, CTCL tumor antigen se2 5, PAR 3, PAR3, PAR3 alpha, PAR3A, PAR3alpha, PARD 3, PARD3, PARD3A, SE2 5L16, SE2 5LT1, SE2 5T2

Tested Applications

Positive WB detected inA549 cells, MCF-7 cells, HEK-293 cells, HeLa cells
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells, mouse brain tissue, mouse kidney tissue
Positive FC detected inMCF-7 cells

Recommended dilution

Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:200
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11085-1-AP targets PARD3 in WB, IP, IF, FC, IHC, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat, chicken
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PARD3 fusion protein Ag1565
Full Name par-3 partitioning defective 3 homolog (C. elegans)
Calculated Molecular Weight 151 kDa
Observed Molecular Weight 180 kDa, 140-150 kDa, 100 kDa
GenBank Accession NumberBC011711
Gene Symbol PARD3
Gene ID (NCBI) 56288
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PARD3 (also known as ASIP, Par3, or Bazooka) is one of PARD proteins which are essential for asymmetric cell division and polarized growth. PARD3 is involved in the establishment of cell polarity and in the asymmetric cytokinesis. It plays a role in tight junctions at epithelial cell-cell contacts. PARD3 has three splice isoforms at 100 kDa, 150 kDa, and 180 kDa. This polyclonal antibody raised against C-terminal 281 amino acids of human PARD3 recognizes these three isoforms.


Product Specific Protocols
WB protocol for PARD3 antibody 11085-1-APDownload protocol
IHC protocol for PARD3 antibody 11085-1-APDownload protocol
IF protocol for PARD3 antibody 11085-1-APDownload protocol
IP protocol for PARD3 antibody 11085-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Nat Commun

EMX2-GPR156-Gαi reverses hair cell orientation in mechanosensory epithelia.

Authors - Katie S Kindt

Matrix Biol

Ameloblastin promotes polarization of ameloblast cell lines in a 3-D cell culture system

Authors - Gayathri Visakan


Coupling of apical-basal polarity and PCP to interpret the Wnt signaling gradient and orient feather branch.

Authors - Jianqiong Lin
  • KD Validated


Perturbation of epithelial apicobasal polarity by rhomboid family-1 gene overexpression.

Authors - Xue-Mei Peng


Glucocorticoid receptor-mediated Nr1d1 chromatin circadian misalignment in stress-induced irritable bowel syndrome

Authors - Gen Zheng

Front Cell Dev Biol

Nox1-based NADPH oxidase regulates the Par protein complex activity to control cell polarization

Authors - Alejandra Valdivia


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Sarah (Verified Customer) (06-26-2019)

Western blot: Total cell lysate (15 ug) was resolved on a 4-12% Bis-Tris gel and transferred to nitrocellulose membrane. Membrane was incubated in blocking buffer (5% milk/0.1% Tween-20) for 1h. Membrane was incubated with anti-PARD-3 in blocking buffer (1:1000) at 4C overnight. After washing, membrane was incubated in anti-rabbit-HRP in blocking bufffer (1:3000) for 1h at room temperature. Protein was detected using ECL reagent and imaged on a chemiluminescence detection system.Immunofluorescence: Cells fixed in MeOH at -20 degrees were stained with 1:200 primary antibody. After washing, coverslips were incubated in 1:500 AF488 secondary antibody. Following mounting, images were acquired using a confocal microscope. Staining was very weak, as is seen by the image (Green = PARD3, Blue = Hoescht)

  • Applications: Western Blot, Immunofluorescence,
  • Primary Antibody Dilution: 1:1000 (WB), 1:200 (IF)
  • Cell Tissue Type: MCF10A
PARD3 Antibody Western Blot,Immunofluorescence, validation (1:1000 (WB), 1:200 (IF) dilution) in MCF10A (Cat no:11085-1-AP)