PARD3 Polyclonal antibody

• Featured Product
• KD/KO Validated

PARD3 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

Publications

23

Reviews (1)

Cat no : 11085-1-AP

Synonyms

ASIP, Baz, Bazooka, CTCL tumor antigen se2 5, PAR 3, PAR3, PAR3 alpha, PAR3A, PAR3alpha, PARD 3, PARD3, PARD3A, SE2 5L16, SE2 5LT1, SE2 5T2

---

Validation Data Gallery

• 

  WB analysis using 11085-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11085-1-AP (PARD3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 11085-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 11085-1-AP (PARD3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of MCF-7 using 11085-1-AP

  IP Result of anti-PARD3 (IP:11085-1-AP, 4ug; Detection:11085-1-AP 1:200) with MCF-7 cells lysate 2900ug.

• 

  IHC staining of human colon cancer using 11085-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11085-1-AP (PARD3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon cancer using 11085-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11085-1-AP (PARD3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of MCF-7 using 11085-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using PARD3 antibody (11085-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse kidney using 11085-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using 11085-1-AP (PARD3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 11085-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 11085-1-AP (PARD3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of MCF-7 using 11085-1-AP

  1X10^6 MCF-7 cells were stained with 0.2ug PARD3 antibody (11085-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

---

Tested Applications

• Positive WB detected in: A549 cells, MCF-7 cells, HEK-293 cells, HeLa cells
• Positive IP detected in: MCF-7 cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: MCF-7 cells, mouse brain tissue, mouse kidney tissue
• Positive FC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:50-1:200
• Immunofluorescence (IF): IF : 1:200-1:800

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 3 publications below
• WB: See 9 publications below
• IHC: See 4 publications below
• IF: See 11 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Product Information

11085-1-AP targets PARD3 in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat, chicken
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PARD3 fusion protein Ag1565
• Full Name: par-3 partitioning defective 3 homolog (C. elegans)
• Calculated molecular weight: 151 kDa
• Observed molecular weight: 180 kDa, 140-150 kDa, 100 kDa
• GenBank accession number: BC011711
• Gene symbol: PARD3
• Gene ID (NCBI): 56288
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PARD3 (also known as ASIP, Par3, or Bazooka) is one of PARD proteins which are essential for asymmetric cell division and polarized growth. PARD3 is involved in the establishment of cell polarity and in the asymmetric cytokinesis. It plays a role in tight junctions at epithelial cell-cell contacts. PARD3 has three splice isoforms at 100 kDa, 150 kDa, and 180 kDa. This polyclonal antibody raised against C-terminal 281 amino acids of human PARD3 recognizes these three isoforms.

Publications

Species	Application	Title
mouse	IF	Nat Commun; EMX2-GPR156-Gαi reverses hair cell orientation in mechanosensory epithelia.; Authors - Katie S Kindt
mouse	IF	Matrix Biol; Ameloblastin promotes polarization of ameloblast cell lines in a 3-D cell culture system; Authors - Gayathri Visakan
chicken	IHC,IF	Development; Coupling of apical-basal polarity and PCP to interpret the Wnt signaling gradient and orient feather branch.; Authors - Jianqiong Lin ; KD Validated
		FASEB J; Perturbation of epithelial apicobasal polarity by rhomboid family-1 gene overexpression.; Authors - Xue-Mei Peng
mouse	WB	iScience; Glucocorticoid receptor-mediated Nr1d1 chromatin circadian misalignment in stress-induced irritable bowel syndrome; Authors - Gen Zheng
mouse	IP	Front Cell Dev Biol; Nox1-based NADPH oxidase regulates the Par protein complex activity to control cell polarization; Authors - Alejandra Valdivia

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Sarah (Verified Customer) (06-26-2019)

Western blot: Total cell lysate (15 ug) was resolved on a 4-12% Bis-Tris gel and transferred to nitrocellulose membrane. Membrane was incubated in blocking buffer (5% milk/0.1% Tween-20) for 1h. Membrane was incubated with anti-PARD-3 in blocking buffer (1:1000) at 4C overnight. After washing, membrane was incubated in anti-rabbit-HRP in blocking bufffer (1:3000) for 1h at room temperature. Protein was detected using ECL reagent and imaged on a chemiluminescence detection system.Immunofluorescence: Cells fixed in MeOH at -20 degrees were stained with 1:200 primary antibody. After washing, coverslips were incubated in 1:500 AF488 secondary antibody. Following mounting, images were acquired using a confocal microscope. Staining was very weak, as is seen by the image (Green = PARD3, Blue = Hoescht)

• Applications: Western Blot, Immunofluorescence,
• Primary Antibody Dilution: 1:1000 (WB), 1:200 (IF)
• Cell Tissue Type: MCF10A

PARD3 Antibody Western Blot,Immunofluorescence, validation (1:1000 (WB), 1:200 (IF) dilution) in MCF10A (Cat no:11085-1-AP)

Related Products

PARD6B Polyclonal antibody

13996-1-AP

PKC Zeta Polyclonal antibody

10860-1-AP

LIMK2 Polyclonal antibody

12350-1-AP

CDC42 Polyclonal antibody

10155-1-AP

PTEN Polyclonal antibody

22034-1-AP

PTEN Monoclonal antibody

60300-1-Ig