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  • KD/KO Validated

PARD3 Polyclonal antibody

PARD3 Polyclonal Antibody for FC, IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, FC,ELISA

Conjugate

Unconjugated

Cat no : 11085-1-AP

Synonyms

ASIP, Baz, Bazooka, CTCL tumor antigen se2 5, PAR 3, PAR3, PAR3 alpha, PAR3A, PAR3alpha, PARD 3, PARD3, PARD3A, SE2 5L16, SE2 5LT1, SE2 5T2



Tested Applications

Positive WB detected inMCF-7 cells
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells, mouse brain tissue, mouse kidney tissue
Positive FC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

11085-1-AP targets PARD3 in WB, IP, IHC, IF, FC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity chicken, human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PARD3 fusion protein Ag1565
Full Name par-3 partitioning defective 3 homolog (C. elegans)
Calculated molecular weight 151 kDa
Observed molecular weight 180 kDa, 140-150 kDa, 100 kDa
GenBank accession numberBC011711
Gene symbol PARD3
Gene ID (NCBI) 56288
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PARD3 (also known as ASIP, Par3, or Bazooka) is one of PARD proteins which are essential for asymmetric cell division and polarized growth. PARD3 is involved in the establishment of cell polarity and in the asymmetric cytokinesis. It plays a role in tight junctions at epithelial cell-cell contacts. PARD3 has three splice isoforms at 100 kDa, 150 kDa, and 180 kDa. This polyclonal antibody raised against C-terminal 281 amino acids of human PARD3 recognizes these three isoforms.

Protocols

Product Specific Protocols
WB protocol for PARD3 antibody 11085-1-APDownload protocol
IHC protocol for PARD3 antibody 11085-1-APDownload protocol
IF protocol for PARD3 antibody 11085-1-APDownload protocol
IP protocol for PARD3 antibody 11085-1-APDownload protocol
FC protocol for PARD3 antibody 11085-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Dev Growth Differ

Overexpression of EphB4 in the mammary epithelium shifts the differentiation pathway of progenitor cells and promotes branching activity and vascularization.

Authors - Philip Kaenel
chickenIHC,IF

Development

Coupling of apical-basal polarity and PCP to interpret the Wnt signaling gradient and orient feather branch.

Authors - Jianqiong Lin
  • KD Validated

FASEB J

Perturbation of epithelial apicobasal polarity by rhomboid family-1 gene overexpression.

Authors - Xue-Mei Peng
WB

Theriogenology

Influence of Wilms' tumor suppressor gene WT1 on bovine Sertoli cells polarity and tight junctions via non-canonical WNT signaling pathway.

Authors - Xue Wang
mouseWB

J Mol Histol

AhR activation protects intestinal epithelial barrier function through regulation of Par-6.

Authors - Kun Yu
mouseIHC

Int J Oncol

Deregulated ephrin-B2 signaling in mammary epithelial cells alters the stem cell compartment and interferes with the epithelial differentiation pathway.

Authors - Kaenel Philip P

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Sarah (Verified Customer) (06-26-2019)

Western blot: Total cell lysate (15 ug) was resolved on a 4-12% Bis-Tris gel and transferred to nitrocellulose membrane. Membrane was incubated in blocking buffer (5% milk/0.1% Tween-20) for 1h. Membrane was incubated with anti-PARD-3 in blocking buffer (1:1000) at 4C overnight. After washing, membrane was incubated in anti-rabbit-HRP in blocking bufffer (1:3000) for 1h at room temperature. Protein was detected using ECL reagent and imaged on a chemiluminescence detection system.Immunofluorescence: Cells fixed in MeOH at -20 degrees were stained with 1:200 primary antibody. After washing, coverslips were incubated in 1:500 AF488 secondary antibody. Following mounting, images were acquired using a confocal microscope. Staining was very weak, as is seen by the image (Green = PARD3, Blue = Hoescht)

  • Applications: Western Blot,Immunofluorescence,
  • Primary Antibody Dilution: 1:1000 (WB), 1:200 (IF)
  • Cell Tissue Type: MCF10A
PARD3 Antibody Western Blot,Immunofluorescence, validation (1:1000 (WB), 1:200 (IF) dilution) in MCF10A (Cat no:11085-1-AP)