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- KD/KO Validated
PARD3 Polyclonal antibody
PARD3 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (2)
Applications
WB, IP, IHC, IF, FC, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 11085-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | A549 cells, MCF-7 cells, HEK-293 cells, HeLa cells |
| Positive IP detected in | MCF-7 cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | MCF-7 cells, mouse brain tissue, mouse kidney tissue |
| Positive FC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:50-1:200 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 8 publications below |
| IHC | See 4 publications below |
| IF | See 11 publications below |
| CoIP | See 1 publications below |
Product Information
11085-1-AP targets PARD3 in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat, chicken |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | PARD3 fusion protein Ag1565 |
| Full Name | par-3 partitioning defective 3 homolog (C. elegans) |
| Calculated molecular weight | 151 kDa |
| Observed molecular weight | 180 kDa, 140-150 kDa, 100 kDa |
| GenBank accession number | BC011711 |
| Gene symbol | PARD3 |
| Gene ID (NCBI) | 56288 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PARD3 (also known as ASIP, Par3, or Bazooka) is one of PARD proteins which are essential for asymmetric cell division and polarized growth. PARD3 is involved in the establishment of cell polarity and in the asymmetric cytokinesis. It plays a role in tight junctions at epithelial cell-cell contacts. PARD3 has three splice isoforms at 100 kDa, 150 kDa, and 180 kDa. This polyclonal antibody raised against C-terminal 281 amino acids of human PARD3 recognizes these three isoforms.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PARD3 antibody 11085-1-AP | Download protocol |
| IHC protocol for PARD3 antibody 11085-1-AP | Download protocol |
| IF protocol for PARD3 antibody 11085-1-AP | Download protocol |
| IP protocol for PARD3 antibody 11085-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Matrix Biol Ameloblastin promotes polarization of ameloblast cell lines in a 3-D cell culture system | ||
Sci Rep Deletion of Brg1 causes abnormal hair cell planer polarity, hair cell anchorage, and scar formation in mouse cochlea. | ||
Hum Mol Genet Loss of BAF (mSWI/SNF) chromatin-remodeling ATPase Brg1 causes multiple malformations of cortical development in mice. | ||
Biomed Pharmacother Par3 promotes breast cancer invasion and migration through pull tension and protein nanoparticle-induced osmotic pressure | ||
J Cell Mol Med PARD3 gene variation as candidate cause of nonsyndromic cleft palate only. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Sarah (Verified Customer) (06-26-2019) | Western blot: Total cell lysate (15 ug) was resolved on a 4-12% Bis-Tris gel and transferred to nitrocellulose membrane. Membrane was incubated in blocking buffer (5% milk/0.1% Tween-20) for 1h. Membrane was incubated with anti-PARD-3 in blocking buffer (1:1000) at 4C overnight. After washing, membrane was incubated in anti-rabbit-HRP in blocking bufffer (1:3000) for 1h at room temperature. Protein was detected using ECL reagent and imaged on a chemiluminescence detection system.Immunofluorescence: Cells fixed in MeOH at -20 degrees were stained with 1:200 primary antibody. After washing, coverslips were incubated in 1:500 AF488 secondary antibody. Following mounting, images were acquired using a confocal microscope. Staining was very weak, as is seen by the image (Green = PARD3, Blue = Hoescht)
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