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PARD3 Polyclonal antibody
PARD3 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Cat no : 11085-1-AP
Validation Data Gallery
|Positive WB detected in||A549 cells, MCF-7 cells, HEK-293 cells, HeLa cells|
|Positive IP detected in||MCF-7 cells|
|Positive IHC detected in||human colon cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||MCF-7 cells, mouse brain tissue, mouse kidney tissue|
|Positive FC detected in||MCF-7 cells|
|Western Blot (WB)||WB : 1:2000-1:10000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:200|
|Immunofluorescence (IF)||IF : 1:200-1:800|
|Sample-dependent, check data in validation data gallery|
11085-1-AP targets PARD3 in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse, rat, chicken|
|Host / Isotype||Rabbit / IgG|
|Immunogen||PARD3 fusion protein Ag1565|
|Full Name||par-3 partitioning defective 3 homolog (C. elegans)|
|Calculated molecular weight||151 kDa|
|Observed molecular weight||180 kDa, 140-150 kDa, 100 kDa|
|GenBank accession number||BC011711|
|Gene ID (NCBI)||56288|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
PARD3 (also known as ASIP, Par3, or Bazooka) is one of PARD proteins which are essential for asymmetric cell division and polarized growth. PARD3 is involved in the establishment of cell polarity and in the asymmetric cytokinesis. It plays a role in tight junctions at epithelial cell-cell contacts. PARD3 has three splice isoforms at 100 kDa, 150 kDa, and 180 kDa. This polyclonal antibody raised against C-terminal 281 amino acids of human PARD3 recognizes these three isoforms.
|Product Specific Protocols|
|WB protocol for PARD3 antibody 11085-1-AP||Download protocol|
|IHC protocol for PARD3 antibody 11085-1-AP||Download protocol|
|IF protocol for PARD3 antibody 11085-1-AP||Download protocol|
|IP protocol for PARD3 antibody 11085-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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Coupling of apical-basal polarity and PCP to interpret the Wnt signaling gradient and orient feather branch.
Perturbation of epithelial apicobasal polarity by rhomboid family-1 gene overexpression.
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Front Cell Dev Biol
Nox1-based NADPH oxidase regulates the Par protein complex activity to control cell polarization
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Sarah (Verified Customer) (06-26-2019)
Western blot: Total cell lysate (15 ug) was resolved on a 4-12% Bis-Tris gel and transferred to nitrocellulose membrane. Membrane was incubated in blocking buffer (5% milk/0.1% Tween-20) for 1h. Membrane was incubated with anti-PARD-3 in blocking buffer (1:1000) at 4C overnight. After washing, membrane was incubated in anti-rabbit-HRP in blocking bufffer (1:3000) for 1h at room temperature. Protein was detected using ECL reagent and imaged on a chemiluminescence detection system.Immunofluorescence: Cells fixed in MeOH at -20 degrees were stained with 1:200 primary antibody. After washing, coverslips were incubated in 1:500 AF488 secondary antibody. Following mounting, images were acquired using a confocal microscope. Staining was very weak, as is seen by the image (Green = PARD3, Blue = Hoescht)