PARD6B Polyclonal antibody

PARD6B Polyclonal Antibody for FC, IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC,ELISA

Conjugate

Unconjugated

Cat no : 13996-1-AP

Synonyms

PAR 6 beta, PAR 6B, PAR6B, PARD6B



Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, human placenta tissue, PC-3 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inmouse kidney tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, MCF-7 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 2 publications below

Product Information

13996-1-AP targets PARD6B in WB, IP, IHC, IF, FC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PARD6B fusion protein Ag5083
Full Name par-6 partitioning defective 6 homolog beta (C. elegans)
Calculated molecular weight 41 kDa
Observed molecular weight 50-53 kDa
GenBank accession numberBC060847
Gene symbol PARD6B
Gene ID (NCBI) 84612
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PARD6B (also named as PAR6B) is a member of the PAR6 family. PARD6B is known to play a key role in mammary epithelial cell biology. Several studies have been reported which indicate deregulated PARD6B signaling contributes to malignant epithelial cell phenotypes due predominantly to disrupted polymerization and maintenance of tight junctions (PMID: 22957302). The approximately 4.8-kb long PARD6B mRNA was predominantly detected in both adult and foetal kidneys, while much weaker but significant signals were observed in the placenta, lung and liver (PMID: 11260256). This antibody detects PARD6B with an apparent molecular weight of 50-53 kDa as has been demonstrated by several researches (PMID: 22496418; 25662318).

Protocols

Product Specific Protocols
WB protocol for PARD6B antibody 13996-1-APDownload protocol
IHC protocol for PARD6B antibody 13996-1-APDownload protocol
IF protocol for PARD6B antibody 13996-1-APDownload protocol
IP protocol for PARD6B antibody 13996-1-APDownload protocol
FC protocol for PARD6B antibody 13996-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Theriogenology

Influence of Wilms' tumor suppressor gene WT1 on bovine Sertoli cells polarity and tight junctions via non-canonical WNT signaling pathway.

Authors - Xue Wang
mouseWB

PLoS One

PKCζ and JNK signaling regulate radiation-induced compensatory proliferation in parotid salivary glands.

Authors - Wen Yu Wong