PARD6B Polyclonal antibody

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• PARD6B Polyclonal antibody (13996-1-AP)

PARD6B Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 13996-1-AP Copied!

Publications (9)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

PAR-6 beta, PAR6B, PAR-6B, Partitioning defective 6 homolog beta

• 

  WB analysis of HeLa using 13996-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13996-1-AP (PARD6B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 13996-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13996-1-AP (PARD6B antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human placenta using 13996-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 13996-1-AP (PARD6B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of PC-3 using 13996-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 13996-1-AP (PARD6B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HEK-293 using 13996-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13996-1-AP (PARD6B Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of HEK-293 using 13996-1-AP

  IP result of anti-PARD6B (IP:13996-1-AP, 3ug; Detection:13996-1-AP 1:200) with HEK-293 cells lysate 6000ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse kidney using 13996-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 13996-1-AP (PARD6B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse kidney using 13996-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 13996-1-AP (PARD6B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse kidney using 13996-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 13996-1-AP (PARD6B antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human stomach cancer using 13996-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13996-1-AP (PARD6B antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse liver using 13996-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 13996-1-AP (PARD6B antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of rat kidney using 13996-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 13996-1-AP (PARD6B antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of rat liver using 13996-1-AP

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 13996-1-AP (PARD6B antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human pancreas using 13996-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 13996-1-AP (PARD6B antibody) at dilution of 1:100 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human pancreas using 13996-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 13996-1-AP (PARD6B antibody) at dilution of 1:100 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HepG2 using 13996-1-AP

  Immunofluorescent analysis of HepG2 cells, using PARD6B antibody 13996-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of MCF-7 using 13996-1-AP

  Immunofluorescent analysis of MCF-7 cells, using PARD6B antibody 13996-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, human placenta tissue, PC-3 cells, LNCaP cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: mouse kidney tissue, mouse liver tissue, human pancreas tissue, rat liver tissue, human stomach cancer tissue, rat kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells, MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13996-1-AP targets PARD6B in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag5083

  Product name: Recombinant human PARD6B protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-372 aa of BC060847

  Sequence: MNRSHRHGAGSGCLGTMEVKSKFGAEFRRFSLERSKPGKFEEFYGLLQHVHKIPNVDVLVGYADIHGDLLPINNDDNYHKAVSTANPLLRIFIQKKEEADYSAFGTDTLIKKKNVLTNVLRPDNHRKKPHIVISMPQDFRPVSSIIDVDILPETHRRVRLYKYGTEKPLGFYIRDGSSVRVTPHGLEKVPGIFISRLVPGGLAQSTGLLAVNDEVLEVNGIEVSGKSLDQVTDMMIANSRNLIITVRPANQRNNVVRNSRTSGSSGQSTDNSLLGYPQQIEPSFEPEDEDSEEDDIIIEDNGVPQQIPKAVPNTESLESLTQIELSFESGQNGFIPSNEVSLAAIASSSNTEFETHAPDQKLLEEDGTIITL

• Full Name: par-6 partitioning defective 6 homolog beta (C. elegans)
• Calculated Molecular Weight: 41 kDa
• Observed Molecular Weight: 50-53 kDa
• GenBank Accession Number: BC060847
• Gene Symbol: PARD6B
• Gene ID (NCBI): 84612
• RRID: AB_2159779
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9BYG5
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PARD6B (also named PAR6B) is a member of the PAR6 family. PARD6B is known to play a key role in mammary epithelial cell biology. Several studies have been reported which indicate deregulated PARD6B signaling contributes to malignant epithelial cell phenotypes due predominantly to disrupted polymerization and maintenance of tight junctions (PMID: 22957302). The approximately 4.8-kb long PARD6B mRNA was predominantly detected in both adult and fetal kidneys, while much weaker but significant signals were observed in the placenta, lung, and liver (PMID: 11260256). This antibody detects PARD6B with an apparent molecular weight of 50-53 kDa as has been demonstrated by several researches (PMID: 22496418; 25662318).

Publications

What published studies show

The PARD6B Polyclonal antibody (Cat# 13996-1-AP) has 9 citations published in journals including Cell, Cell Death & Differentiation, Cancer Letters, Stem Cell Research & Therapy, Journal of Agricultural and Food Chemistry, Cancer Gene Therapy, iScience, Theriogenology, and PLoS One. Research fields span early embryo development, mitotic spindle biology, lung cancer, COPD/emphysema, collagen characterization, retinal degeneration, Sertoli cell polarity, and salivary gland proliferation.

Automatically generated from the 9 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IF	Cell; Two distinct causes contribute to the low efficiency of human pre-implantation development.; Authors - Zixuan Li
		Cell Death Differ; USP21-mediated deubiquitylation stimulates NuMA recruitment to the cell cortex to promote mitotic spindle orientation.; Authors - Mingzheng Hu
human	WB,IHC	Cancer Lett; Oncogenic functions and therapeutic potentials of targeted inhibition of SMARCAL1 in small cell lung cancer; Authors - Bei-Bei Sun
mouse,human	WB	Stem Cell Res Ther; The PAR6B-PRKCI-PAR3 complex influences alveolar regeneration in patients with the emphysema subtype of chronic obstructive pulmonary disease; Authors - Di Wang
human	WB	J Agric Food Chem; Discovery and Functional Characterization of a Recombinant Fragment of Human Collagen Type XVII; Authors - Lianhua Piao
		Cancer Gene Ther; PRAD6A promotes lung adenocarcinoma cell proliferation and invasion through Serpina3; Authors - Lanlin Hu