|Positive WB detected in||HeLa cells, HEK-293 cells, human placenta tissue, PC-3 cells|
|Positive IP detected in||HEK-293 cells|
|Positive IHC detected in||mouse kidney tissue, human pancreas tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells, MCF-7 cells|
|Positive FC detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
13996-1-AP targets PARD6B in WB, IP, IHC, IF, FC,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||PARD6B fusion protein Ag5083|
|Full Name||par-6 partitioning defective 6 homolog beta (C. elegans)|
|Calculated molecular weight||41 kDa|
|Observed molecular weight||50-53 kDa|
|GenBank accession number||BC060847|
|Gene ID (NCBI)||84612|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
PARD6B (also named as PAR6B) is a member of the PAR6 family. PARD6B is known to play a key role in mammary epithelial cell biology. Several studies have been reported which indicate deregulated PARD6B signaling contributes to malignant epithelial cell phenotypes due predominantly to disrupted polymerization and maintenance of tight junctions (PMID: 22957302). The approximately 4.8-kb long PARD6B mRNA was predominantly detected in both adult and foetal kidneys, while much weaker but significant signals were observed in the placenta, lung and liver (PMID: 11260256). This antibody detects PARD6B with an apparent molecular weight of 50-53 kDa as has been demonstrated by several researches (PMID: 22496418; 25662318).
|Product Specific Protocols|
|WB protocol for PARD6B antibody 13996-1-AP||Download protocol|
|IHC protocol for PARD6B antibody 13996-1-AP||Download protocol|
|IF protocol for PARD6B antibody 13996-1-AP||Download protocol|
|IP protocol for PARD6B antibody 13996-1-AP||Download protocol|
|FC protocol for PARD6B antibody 13996-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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