PARK7,DJ-1 Polyclonal antibody

• Featured Product
• KD/KO Validated

PARK7,DJ-1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

7

Reviews (1)

Cat no : 11681-1-AP

Synonyms

DJ 1, DJ1, Oncogene DJ1, PARK7, Parkinson disease protein 7, Protein DJ 1

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Validation Data Gallery

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  WB analysis of HeLa using 11681-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11681-1-AP (PARK7,DJ-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 11681-1-AP

  IP Result of anti-PARK7,DJ-1 (IP:11681-1-AP, 4ug; Detection:11681-1-AP 1:1000) with HeLa cells lysate 1200ug.

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  IHC staining of human kidney using 11681-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11681-1-AP (PARK7,DJ-1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human gliomas using 11681-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11681-1-AP (PARK7,DJ-1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of SH-SY5Y using 11681-1-AP

  Immunofluorescent analysis of SH-SY5Y cells using 11681-1-AP (PARK7,DJ-1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, Jurkat cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human gliomas tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:20-1:200

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IF: See 1 publications below

Product Information

11681-1-AP targets PARK7,DJ-1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PARK7,DJ-1 fusion protein Ag2287
• Full Name: Parkinson disease (autosomal recessive, early onset) 7
• Calculated molecular weight: 189 aa, 20 kDa
• Observed molecular weight: 20 kDa, 25 kDa
• GenBank accession number: BC008188
• Gene symbol: PARK7
• Gene ID (NCBI): 11315
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

PARK7, also named as DJ1, belongs to the peptidase C56 family. It protects cells against oxidative stress and cell death. PARK7 plays a role in regulating expression or stability of the mitochondrial uncoupling proteins SLC25A14 and SLC25A27 in dopaminergic neurons of the substantia nigra pars compacta and attenuates the oxidative stress induced by calcium entry into the neurons via L-type channels during pacemaking. It eliminates hydrogen peroxide and protects cells against hydrogen peroxide-induced cell death. PARK7 has cell-growth promoting activity and transforming activity. It may function as a redox-sensitive chaperone. It's precursor undergoes a cleavage of a C-terminal peptide and subsequent activation of protease activity in response to oxidative stress. The amino acid replace at 166 (L → P) reduces PARK7 protein stability and leads to increased degradation. The predicted MW of this protein is 20 kDa, An additional 25 kDa band can be observed due to modification (PMID: 31767755).

Publications

Species	Application	Title
human	WB	Curr Med Sci; DJ-1 Alters Epirubicin-induced Apoptosis via Modulating Epirubicinactivated Autophagy in Human Gastric Cancer Cells.; Authors - Xue-Kai Pan ; KD Validated
human	WB	Evid Based Complement Alternat Med; Mechanistic Study of the Phytocompound, 2- β -D-Glucopyranosyloxy-1-hydroxytrideca-5,7,9,11-tetrayne in Human T-Cell Acute Lymphocytic Leukemia Cells by Using Combined Differential Proteomics and Bioinformatics Approaches.; Authors - Jeng-Yuan Shiau
		Oncol Rep; DJ-1 knockdown inhibits growth and xenograft‑induced tumor generation of human hepatocellular carcinoma cells.; Authors - Shunfang Liu
human	WB	Virulence; Enterovirus 71 induces neural cell apoptosis and autophagy through promoting ACOX1 downregulation and ROS generation.; Authors - Lei You
human, mouse	WB,IF	Mol Cell Biol; ROS-mediated DJ-1 monomerization modulates intracellular trafficking involving Karyopherin β2.; Authors - Benny Björkblom
human	WB	Mol Cell Proteomics; Chemoproteomics Reveals Chemical Diversity and Dynamics of 4-Oxo-2-nonenal Modifications in Cells.; Authors - Rui Sun

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Daniel (Verified Customer) (04-16-2019)

Specific band in RCC4 cells

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human kidney cancer cells (RCC4)

PARK7,DJ-1 Antibody Western Blot, validation (1:1000 dilution) in Human kidney cancer cells (RCC4) (Cat no:11681-1-AP)