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PARK7,DJ-1 Antibody 5 Publications

Rabbit Polyclonal| Catalog number: 11681-1-AP

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Con: 82 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, ELISA
Application not tested? Please see our Guarantee


Images See all

PARK7,DJ-1 Antibody WB HeLa cells 11681-1-AP

HeLa cells were subjected to SDS PAGE followed by western blot with 11681-1-AP(PARK7,DJ-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

PARK7,DJ-1 Antibody IP HeLa cells 11681-1-AP

IP Result of anti-PARK7,DJ-1 (IP:11681-1-AP, 4ug; Detection:11681-1-AP 1:1000) with HeLa cells lysate 1200ug.

PARK7,DJ-1 Antibody IHC human kidney tissue 11681-1-AP

Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11681-1-AP( PARK7,DJ-1 Antibody) at dilution of 1:50 (under 40x lens)

PARK7,DJ-1 Antibody IHC human gliomas tissue 11681-1-AP

Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11681-1-AP( PARK7,DJ-1 Antibody) at dilution of 1:50 (under 40x lens)

PARK7,DJ-1 Antibody IF SH-SY5Y cells 11681-1-AP

Immunofluorescent analysis of SH-SY5Y cells using 11681-1-AP(PARK7,DJ-1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Species specificity:
human, mouse
Positive WB detected in:
HeLa cells, HEK-293 cells, Jurkat cells
Positive IP detected in:
HeLa cells
Positive IHC detected in:
human gliomas tissue, human kidney tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
SH-SY5Y cells
Recommended dilution:
WB : 1:500-1:2000
IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
IHC : 1:20-1:200
IF : 1:20-1:200
Sample-dependent, check data in Validation tab

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
PARK7,DJ-1 fusion protein Ag2287
Full name:
Parkinson disease (autosomal recessive, early onset) 7
Calculated molecular weight:
189aa,20 kDa
Observed molecular weight:
20 kDa, 25 kDa
GenBank accession number:
BC008188
Gene ID (NCBI):
11315
Gene symbol
PARK7
Synonyms
DJ 1, DJ1, Oncogene DJ1, PARK7, Parkinson disease protein 7, Protein DJ 1

Background

PARK7, also named as DJ1, belongs to the peptidase C56 family. It protects cells against oxidative stress and cell death. PARK7 plays a role in regulating expression or stability of the mitochondrial uncoupling proteins SLC25A14 and SLC25A27 in dopaminergic neurons of the substantia nigra pars compacta and attenuates the oxidative stress induced by calcium entry into the neurons via L-type channels during pacemaking. It eliminates hydrogen peroxide and protects cells against hydrogen peroxide-induced cell death. PARK7 has cell-growth promoting activity and transforming activity. It may function as a redox-sensitive chaperone. It's precursor undergoes a cleavage of a C-terminal peptide and subsequent activation of protease activity in response to oxidative stress. The amino acid replace at 166 (L → P) reduces PARK7 protein stability and leads to increased degradation.

Customer Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

Share your scientific insight on this antibody

PARK7,DJ-1 Rabbit Polyclonal antibody

Daniel (Verified Customer), 04-16-2019
Applications
Western Blot,
Primary Antibody Dilution
1:1000
PARK7,DJ-1 Antibody Western Blot, validation (1:1000 dilution) in Human kidney cancer cells (RCC4) (Cat no:11681-1-AP)

PARK7,DJ-1 Antibody Western Blot, validation (1:1000 dilution) in Human kidney cancer cells (RCC4) (Cat no:11681-1-AP)

Cell/Tissue Type
Human kidney cancer cells (RCC4)
Lot Number
Specific band in RCC4 cells

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