|Positive WB detected in||HeLa cells, HEK-293 cells, Jurkat cells|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human gliomas tissue, human kidney tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||SH-SY5Y cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
11681-1-AP targets PARK7,DJ-1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||PARK7,DJ-1 fusion protein Ag2287|
|Full Name||Parkinson disease (autosomal recessive, early onset) 7|
|Calculated molecular weight||189 aa, 20 kDa|
|Observed molecular weight||20 kDa, 25 kDa|
|GenBank accession number||BC008188|
|Gene ID (NCBI)||11315|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
PARK7, also named as DJ1, belongs to the peptidase C56 family. It protects cells against oxidative stress and cell death. PARK7 plays a role in regulating expression or stability of the mitochondrial uncoupling proteins SLC25A14 and SLC25A27 in dopaminergic neurons of the substantia nigra pars compacta and attenuates the oxidative stress induced by calcium entry into the neurons via L-type channels during pacemaking. It eliminates hydrogen peroxide and protects cells against hydrogen peroxide-induced cell death. PARK7 has cell-growth promoting activity and transforming activity. It may function as a redox-sensitive chaperone. It's precursor undergoes a cleavage of a C-terminal peptide and subsequent activation of protease activity in response to oxidative stress. The amino acid replace at 166 (L → P) reduces PARK7 protein stability and leads to increased degradation. The predicted MW of this protein is 20 kDa, An additional 25 kDa band can be observed due to modification (PMID: 31767755).
|Product Specific Protocols|
|WB protocol for PARK7,DJ-1 antibody 11681-1-AP||Download protocol|
|IHC protocol for PARK7,DJ-1 antibody 11681-1-AP||Download protocol|
|IF protocol for PARK7,DJ-1 antibody 11681-1-AP||Download protocol|
|IP protocol for PARK7,DJ-1 antibody 11681-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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Daniel (Verified Customer) (04-16-2019)
Specific band in RCC4 cells