PARP1 Polyclonal antibody

PARP1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (5)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

221

Reviews (1)

Cat no : 13371-1-AP

Synonyms

ADPRT, ADPRT 1, ADPRT1, pADPRT 1, PARP, PARP 1, PARP1, poly (ADP ribose) polymerase 1, Poly [ADP ribose] polymerase 1, Poly[ADP ribose] synthase 1, PPOL

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Validation Data Gallery

• 

  WB analysis using 13371-1-AP

  Jurkat cells (25 μg/lane) were subjected to SDS PAGE followed by western blot with 13371-1-AP (PARP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 13371-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13371-1-AP (PARP1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 13371-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13371-1-AP (PARP1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  WB analysis of HeLa using 13371-1-AP

  Anti-Fas treated HeLa cells were subjected to SDS PAGE followed by western blot with 13371-1-AP (PARP1 Antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.

• 

  IP experiment of K-562 using 13371-1-AP

  IP Result of anti-PARP1 (IP:13371-1-AP, 4ug; Detection:13371-1-AP 1:600) with K-562 cells lysate 5000ug.

• 

  WB analysis of HeLa using 13371-1-AP

  Cobalt Chloride treated HeLa cells were subjected to SDS PAGE followed by western blot with 13371-1-AP (PARP1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of Neuro-2a using 13371-1-AP

  Immunofluorescent analysis of (4% PFA) fixed Neuro-2a cells using 13371-1-AP (PARP1 antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: Jurkat cells, Cobalt Chloride treated HeLa cells, C6 cells, Fas antibody treated HeLa cells
• Positive IP detected in: K-562 cells
• Positive IHC detected in: human liver cancer tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: Neuro-2a cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 211 publications below
• IHC: See 8 publications below
• IF: See 3 publications below
• IP: See 2 publications below

Product Information

13371-1-AP targets PARP1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, M. grisea, monkey, mouse, pig, rat, sheep, swine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PARP1 fusion protein Ag4193
• Full Name: poly (ADP-ribose) polymerase 1
• Calculated molecular weight: 1014 aa, 113 kDa
• Observed molecular weight: 113-116 kDa, 89 kDa
• GenBank accession number: BC037545
• Gene symbol: PARP1
• Gene ID (NCBI): 142
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

PARP1 (poly(ADP-ribose) polymerase 1) is a nuclear enzyme catalyzing the poly(ADP-ribosyl)ation of many key proteins in vivo. The normal function of PARP1 is the routine repair of DNA damage. Activated by DNA strand breaks, the PARP1 is cleaved into an 85 to 89-kDa COOH-terminal fragment and a 24-kDa NH2-terminal peptide by caspases during the apoptotic process. The appearance of PARP fragments is commonly considered as an important biomarker of apoptosis. In addition to caspases, other proteases like calpains, cathepsins, granzymes and matrix metalloproteinases (MMPs) have also been reported to cleave PARP1 and gave rise to fragments ranging from 42-89-kDa. This antibody was generated against the C-terminal region of human PARP1 and it recognizes the full-length as well as the cleavage of the PARP1.

Publications

Species	Application	Title
human	WB,IHC	Cancers (Basel); Antrocin Sensitizes Prostate Cancer Cells to Radiotherapy through Inhibiting PI3K/AKT and MAPK Signaling Pathways.; Authors - Yu-An Chen
human	WB	Med Sci Monit; PMID: 30605443; Authors - Lin Chen
human	WB	J Cell Physiol; Rg3 inhibits gemcitabine-induced lung cancer cell invasiveness through ROS-dependent, NF-κB- and HIF-1α-mediated downregulation of PTX3.; Authors - Bulbul Ahmmed
human	WB	J Cell Mol Med; Tumour necrosis factor-α-induced protein 8-like 2 is a novel regulator of proliferation, migration, and invasion in human rectal adenocarcinoma cells.; Authors - Dong-Dong Wu
human	WB	Toxins (Basel); Autophagy and Apoptosis Interact to Modulate T-2 Toxin-Induced Toxicity in Liver Cells.; Authors - Jing Wu
human	WB	Br J Pharmacol; Potent effects of dioscin against hepatocellular carcinoma through regulating TIGAR-mediated apoptosis, autophagy and DNA damage.; Authors - Zhang Mao

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Brandon (Verified Customer) (02-01-2019)

Detected Full-length and cleaved PARP-1. Also detected a smaller fragment in mouse mouse liver tissue.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500 in 5% skim milk
• Cell Tissue Type: C57BL/6 Mouse liver

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