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PARP1 Polyclonal antibody

PARP1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (5)

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 13371-1-AP

Synonyms

ADPRT, ADPRT 1, ADPRT1, pADPRT 1, PARP, PARP 1, PARP1, poly (ADP ribose) polymerase 1, Poly [ADP ribose] polymerase 1, Poly[ADP ribose] synthase 1, PPOL



Tested Applications

Positive WB detected inJurkat cells, HeLa cells, C6 cells, Fas antibody treated HeLa cells, Cobalt Chloride treated HeLa cells, THP-1 cells
Positive IP detected inK-562 cells
Positive IHC detected inhuman liver cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inNeuro-2a cells, mouse testis tissue, MCF-7 cells
Positive FC detected inK-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

13371-1-AP targets PARP1 in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, canine, Fungus, monkey, pig, sheep
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PARP1 fusion protein Ag4193
Full Name poly (ADP-ribose) polymerase 1
Calculated molecular weight 1014 aa, 113 kDa
Observed molecular weight 113-116 kDa, 89 kDa
GenBank accession numberBC037545
Gene symbol PARP1
Gene ID (NCBI) 142
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PARP1 (poly(ADP-ribose) polymerase 1) is a nuclear enzyme catalyzing the poly(ADP-ribosyl)ation of many key proteins in vivo. The normal function of PARP1 is the routine repair of DNA damage. Activated by DNA strand breaks, the PARP1 is cleaved into an 85 to 89-kDa COOH-terminal fragment and a 24-kDa NH2-terminal peptide by caspases during the apoptotic process. The appearance of PARP fragments is commonly considered as an important biomarker of apoptosis. In addition to caspases, other proteases like calpains, cathepsins, granzymes and matrix metalloproteinases (MMPs) have also been reported to cleave PARP1 and gave rise to fragments ranging from 42-89-kDa. This antibody was generated against the C-terminal region of human PARP1 and it recognizes the full-length as well as the cleavage of the PARP1.

Protocols

Product Specific Protocols
WB protocol for PARP1 antibody 13371-1-APDownload protocol
IHC protocol for PARP1 antibody 13371-1-APDownload protocol
IF protocol for PARP1 antibody 13371-1-APDownload protocol
IP protocol for PARP1 antibody 13371-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Cancer

YTHDF2 mediates the mRNA degradation of the tumor suppressors to induce AKT phosphorylation in N6-methyladenosine-dependent way in prostate cancer.

Authors - Jiangfeng Li
humanWB

ACS Nano

PMID: 31670945

Authors - Ying Xu
mouseWB

Exp Mol Med

Protective effect of hepatocyte-enriched lncRNA-Mir122hg by promoting hepatocyte proliferation in acute liver injury

Authors - Zhenjun Yu
humanWB

Nat Commun

The ATM and ATR kinases regulate centrosome clustering and tumor recurrence by targeting KIFC1 phosphorylation.

Authors - Guangjian Fan
humanWB

Nat Commun

Loss of KLF14 triggers centrosome amplification and tumorigenesis.

Authors - Guangjian Fan
human,mouseWB,IHC

Biomaterials

Tumor-specific nitric oxide generator to amplify peroxynitrite based on highly penetrable nanoparticles for metastasis inhibition and enhanced cancer therapy.

Authors - Menghao Shi

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Ruchi (Verified Customer) (12-09-2022)

The Ab was used for IF at a dilution of 1:100 for staining mice skin wounds. This is a nuclear protein and so required an additional time for fixation (30 minutes in 4% paraformaldehyde) and permeabilization by chilled methanol for 10 minutes. The blocking buffer used was 4% BSA.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: PARP1
  • Cell Tissue Type: Mouse skin
FH

Marina (Verified Customer) (05-30-2022)

In the blot we could detect native PARP1 as well as its cleaved 89 kDa form after PARP inhibitor treatment

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Human breast cancer cells
PARP1 Antibody Western Blot validation (1:500 dilution) in Human breast cancer cells (Cat no:13371-1-AP)
FH

Huanzhou (Verified Customer) (04-08-2022)

This product works well for WB.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: EBV-transformed lymphoblastoid B-cell lines
FH

Azita (Verified Customer) (06-02-2021)

Western blot analysis using PARP1 polyclonal antibody in NSC34 cell line at dilution of 1:500.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: NSC34
FH

Brandon (Verified Customer) (02-01-2019)

Detected Full-length and cleaved PARP-1. Also detected a smaller fragment in mouse mouse liver tissue.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:500 in 5% skim milk
  • Cell Tissue Type: C57BL/6 Mouse liver