PARP1 Polyclonal antibody
PARP1 Polyclonal Antibody for FC, IF, IHC, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (4)
Applications
WB, IP, IHC, IF, FC,ELISA
Conjugate
Unconjugated
268
Cat no : 13371-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | Jurkat cells, C6 cells, Fas antibody treated HeLa cells, Cobalt Chloride treated HeLa cells |
| Positive IP detected in | K-562 cells |
| Positive IHC detected in | human liver cancer tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | Neuro-2a cells |
| Positive FC detected in | K-562 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 258 publications below |
| IHC | See 9 publications below |
| IF | See 4 publications below |
| IP | See 2 publications below |
Product Information
13371-1-AP targets PARP1 in WB, IP, IHC, IF, FC,ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | Fungus, human, monkey, mouse, pig, rat, sheep |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | PARP1 fusion protein Ag4193 |
| Full Name | poly (ADP-ribose) polymerase 1 |
| Calculated molecular weight | 1014 aa, 113 kDa |
| Observed molecular weight | 113-116 kDa, 89 kDa |
| GenBank accession number | BC037545 |
| Gene symbol | PARP1 |
| Gene ID (NCBI) | 142 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PARP1 (poly(ADP-ribose) polymerase 1) is a nuclear enzyme catalyzing the poly(ADP-ribosyl)ation of many key proteins in vivo. The normal function of PARP1 is the routine repair of DNA damage. Activated by DNA strand breaks, the PARP1 is cleaved into an 85 to 89-kDa COOH-terminal fragment and a 24-kDa NH2-terminal peptide by caspases during the apoptotic process. The appearance of PARP fragments is commonly considered as an important biomarker of apoptosis. In addition to caspases, other proteases like calpains, cathepsins, granzymes and matrix metalloproteinases (MMPs) have also been reported to cleave PARP1 and gave rise to fragments ranging from 42-89-kDa. This antibody was generated against the C-terminal region of human PARP1 and it recognizes the full-length as well as the cleavage of the PARP1.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PARP1 antibody 13371-1-AP | Download protocol |
| IHC protocol for PARP1 antibody 13371-1-AP | Download protocol |
| IF protocol for PARP1 antibody 13371-1-AP | Download protocol |
| IP protocol for PARP1 antibody 13371-1-AP | Download protocol |
| FC protocol for PARP1 antibody 13371-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cancers (Basel) Antrocin Sensitizes Prostate Cancer Cells to Radiotherapy through Inhibiting PI3K/AKT and MAPK Signaling Pathways. | ||
J Cell Physiol Rg3 inhibits gemcitabine-induced lung cancer cell invasiveness through ROS-dependent, NF-κB- and HIF-1α-mediated downregulation of PTX3. | ||
J Cell Mol Med Tumour necrosis factor-α-induced protein 8-like 2 is a novel regulator of proliferation, migration, and invasion in human rectal adenocarcinoma cells. | ||
Toxins (Basel) Autophagy and Apoptosis Interact to Modulate T-2 Toxin-Induced Toxicity in Liver Cells. | ||
Br J Pharmacol Potent effects of dioscin against hepatocellular carcinoma through regulating TIGAR-mediated apoptosis, autophagy and DNA damage. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Azita (Verified Customer) (06-02-2021) | Western blot analysis using PARP1 polyclonal antibody in NSC34 cell line at dilution of 1:500.
|
FH Brandon (Verified Customer) (02-01-2019) | Detected Full-length and cleaved PARP-1. Also detected a smaller fragment in mouse mouse liver tissue.
|
