ChromoTek PARP1-Chromobody® plasmid (TagGFP)

DNA plasmid encoding for anti-PARP1 VHH (anti-PARP1 Nanobody) fused to TagGFP2.




IF, Live cell imaging

Vector type

Mammalian expression vector



Cat no : xcg


Chromobody, PARP1, PARP1 Chromobody

Product Information

DNA plasmid encoding for anti-PARP1 VHH (anti-PARP1 Nanobody) fused to TagGFP2.

DescriptionThe PARP1-Chromobody visualizes endogenous PARP1 in real time in live cells.

• Easily transfect your cells

• Use PARP1-Chromobody® as marker of DNA damage after microirradiation

• PARP1-Chromobody® does not affect cell viability

ApplicationsLive cell imaging
SpecificityPoly(ADP-ribose) polymerase 1 (PARP1), tested in human cells.
Does not bind to PARP2, PARP3, andPARP 9
Vector typeMammalian expression vector
PromoterConstitutive CMV IE
Codon usageMammalian
SequenceWith the PARP1-Chromobody plasmid you receive the sequence information of the alpaca antibody to PARP1 fused to TagGFP2 or TagRFP, as well as the full vector sequence.
TransfectionTransfection of Chromobody plasmids into mammalian cells can be done with standard DNA-transfection methods, e.g. lipofection (Lipofectamine 2000® from Thermo Fisher Scientific), according to the manufacturer’s protocol for the transfection reagent. Please choose the transfection method that works the best for your cell type.
Microscopy techniquesWide-field epifluorescence microscopy; confocal microscopy
Storage ConditionShipped at ambient temperature. Store at -20 °C



Nat Commun

Serine-linked PARP1 auto-modification controls PARP inhibitor response.

Authors - Evgeniia Prokhorova

Biophys J

Q-FADD: A Mechanistic Approach for Modeling the Accumulation of Proteins at Sites of DNA Damage.

Authors - Jyothi Mahadevan