ChromoTek PARP1-Chromobody® plasmid (TagGFP)

DNA plasmid encoding for anti-PARP1 VHH (anti-PARP1 Nanobody) fused to TagGFP2.

Vector type

Mammalian expression vector

Promoter

Constitutive CMV IE

Reporter

TagGFP2

Epitope

PARP1

Cat no : xcg

Synonyms

Chromobody, PARP1, PARP1 Chromobody



Product Information

DNA plasmid encoding for anti-PARP1 VHH (anti-PARP1 Nanobody) fused to TagGFP2.

ApplicationsLive cell imaging
SpecificityPoly(ADP-ribose) polymerase 1 (PARP1), tested in human cells.
Does not bind to PARP2, PARP3, andPARP 9
Vector typeMammalian expression vector
PromoterConstitutive CMV IE
ReporterTagGFP2
Codon usageMammalian
SelectionKan/Neo
Encoded Nanobody/ VHHMonoclonal anti-PARP1 single domain antibody (sdAb) fragment
SequenceWith the PARP1-Chromobody plasmid you receive the sequence information of the alpaca antibody to PARP1 fused to TagGFP2 or TagRFP, as well as the full vector sequence.
Microscopy techniquesWide-field epifluorescence microscopy; confocal microscopy
TransfectionTransfection of Chromobody plasmids into mammalian cells can be done with standard DNA-transfection methods, e.g. lipofection (Lipofectamine 2000® from Thermo Fisher Scientific), according to the manufacturer’s protocol for the transfection reagent. Please choose the transfection method that works the best for your cell type.
Storage ConditionShipped at ambient temperature. Store at -20 °C

Publications

ApplicationTitle

Biophys J

Q-FADD: A Mechanistic Approach for Modeling the Accumulation of Proteins at Sites of DNA Damage.

Authors - Jyothi Mahadevan

Nat Commun

Serine-linked PARP1 auto-modification controls PARP inhibitor response.

Authors - Evgeniia Prokhorova