Cat no : xcr
DNA plasmid encoding for anti-PARP1 VHH (anti-PARP1 Nanobody) fused to TagRFP.
|Description||The PARP1-Chromobody visualizes endogenous PARP1 in real time in live cells.
• Easily transfect your cells
• Use PARP1-Chromobody® as marker of DNA damage after microirradiation
• PARP1-Chromobody® does not affect cell viability
|Applications||Live cell imaging|
|Specificity||Poly(ADP-ribose) polymerase 1 (PARP1), tested in human cells.|
Does not bind to PARP2, PARP3, andPARP 9
|Vector type||Mammalian expression vector|
|Promoter||Constitutive CMV IE|
|Sequence||With the PARP1-Chromobody plasmid you receive the sequence information of the alpaca antibody to PARP1 fused to TagGFP2 or TagRFP, as well as the full vector sequence.|
|Transfection||Transfection of Chromobody plasmids into mammalian cells can be done with standard DNA-transfection methods, e.g. lipofection (Lipofectamine 2000® from Thermo Fisher Scientific), according to the manufacturer’s protocol for the transfection reagent. Please choose the transfection method that works the best for your cell type.|
|Microscopy techniques||Wide-field epifluorescence microscopy; confocal microscopy|
|Storage Condition||Shipped at ambient temperature. Store at -20 °C|
|SDS PARP1--Chromobody® TagGFP plasmid (PDF)|
|Datasheet PARP1-Chromobody® TagGFP plasmid (PDF)|
|Datasheet PARP1-Chromobody® TagRFP plasmid (PDF)|
|Chromotek nanobodies brochure (PDF)|
|End User License Agreement EULA PARP1-Chromobody TagGFP plasmid non-profit (PDF)|
|End User License Agreement EULA PARP1-Chromobody TagRFP plasmid non-profit (PDF)|
SPINDOC binds PARP1 to facilitate PARylation.
Proc Natl Acad Sci U S A
Rapid recruitment of p53 to DNA damage sites directs DNA repair choice and integrity.
J Cell Sci
Local inhibition of rRNA transcription without nucleolar segregation after targeted ion irradiation of the nucleolus.
Chromatin nanoscale compaction in live cells visualized by acceptor-to-donor ratio corrected Förster resonance energy transfer between DNA dyes.