ChromoTek PARP1-Chromobody® plasmid (TagRFP)

DNA plasmid encoding for anti-PARP1 VHH (anti-PARP1 Nanobody) fused to TagRFP.

Vector type

Mammalian expression vector

Promoter

Constitutive CMV IE

Reporter

TagRFP

Epitope

PARP1

Cat no : xcr

Synonyms



Product Information

DNA plasmid encoding for anti-PARP1 VHH (anti-PARP1 Nanobody) fused to TagRFP.

Applications Live cell imaging
SpecificityPoly(ADP-ribose) polymerase 1 (PARP1), tested in human cells.
Does not bind to PARP2, PARP3, andPARP 9
Vector typeMammalian expression vector
PromoterConstitutive CMV IE
ReporterTagRFP
Codon usageMammalian
SelectionKan/Neo
Encoded Nanobody/ VHHMonoclonal anti-PARP1 single domain antibody (sdAb) fragment
SequenceWith the PARP1-Chromobody plasmid you receive the sequence information of the alpaca antibody to PARP1 fused to TagGFP2 or TagRFP, as well as the full vector sequence.
Microscopy techniquesWide-field epifluorescence microscopy; confocal microscopy
TransfectionTransfection of Chromobody plasmids into mammalian cells can be done with standard DNA-transfection methods, e.g. lipofection (Lipofectamine 2000® from Thermo Fisher Scientific), according to the manufacturer’s protocol for the transfection reagent. Please choose the transfection method that works the best for your cell type.
StorageShipped at ambient temperature. Store at -20 °C

Publications

ApplicationTitle
IF

J Biophotonics

Chromatin nanoscale compaction in live cells visualized by acceptor-to-donor ratio corrected Förster resonance energy transfer between DNA dyes.

Authors - Simone Pelicci
IF

J Cell Sci

Local inhibition of rRNA transcription without nucleolar segregation after targeted ion irradiation of the nucleolus.

Authors - Christian Siebenwirth