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  • KD/KO Validated

PARP9 Polyclonal antibody

PARP9 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 17535-1-AP

Synonyms

B aggressive lymphoma protein, BAL, BAL1, PARP 9, PARP9, Poly [ADP ribose] polymerase 9



Tested Applications

Positive WB detected inHeLa cells, K-562 cells, MCF-7 cells, rat skeletal muscle tissue
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman spleen tissue, human skin tissue, human kidney tissue, human heart tissue, human lung tissue, human ovary tissue, human lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

17535-1-AP targets PARP9 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Cited Reactivity human, tree threw
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PARP9 fusion protein Ag11587
Full Name poly (ADP-ribose) polymerase family, member 9
Calculated molecular weight 819 aa, 92 kDa
Observed molecular weight 88 kDa
GenBank accession numberBC039580
Gene symbol PARP9
Gene ID (NCBI) 83666
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Poly(ADP-ribosyl)ation is a post-translational modification of proteins mediated by one of the 17 members of the poly(ADP-ribose) polymerases (PARP). PARP-9 belongs to the subfamily of macroPARPs, associating one to three macro domains to the PARP domain. Overexpression of PARP-9 stimulates cell migration in vitro, suggesting a role for PARP-9 in the promotion of malignant B cell migration and dissemination in high risk DLBCL. PARP-9 is also likely a transcription coactivator, its overexpression in B lymphocytes, stimulated by IFNγ, inducing the transcription of IFNγ-controlled genes.

Protocols

Product Specific Protocols
WB protocol for PARP9 antibody 17535-1-APDownload protocol
IHC protocol for PARP9 antibody 17535-1-APDownload protocol
IP protocol for PARP9 antibody 17535-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Oncol Lett

PARP9 is overexpressed in human breast cancer and promotes cancer cell migration.

Authors - Xinghong Tang
  • KD Validated
tree threwIF,WB

J Immunol

An Alternative Splicing of Tupaia STING Modulated Anti-RNA Virus Responses by Targeting MDA5-LGP2 and IRF3.

Authors - Ling Xu
  • KD Validated
humanWB

Mol Ther

PMID: 29456019

Authors - Xinyi Wang
humanWB,IF

Mol Cell

Ubiquitin Modification by the E3 Ligase/ADP-Ribosyltransferase Dtx3L/Parp9.

Authors - Chun-Song Yang