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- KD/KO Validated
PARP9 Polyclonal Antibody for IHC, IP, WB, ELISA
Cat no : 17535-1-AP
B aggressive lymphoma protein, BAL, BAL1, PARP 9, PARP9, Poly [ADP ribose] polymerase 9
|Positive WB detected in||HeLa cells, K-562 cells, MCF-7 cells, rat skeletal muscle tissue|
|Positive IP detected in||MCF-7 cells|
|Positive IHC detected in||human spleen tissue, human skin tissue, human kidney tissue, human heart tissue, human lung tissue, human ovary tissue, human lymphoma tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
17535-1-AP targets PARP9 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, rat samples.
|Tested Reactivity||human, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||PARP9 fusion protein Ag11587|
|Full Name||poly (ADP-ribose) polymerase family, member 9|
|Calculated molecular weight||819aa,92 kDa|
|Observed molecular weight||88 kDa|
|GenBank accession number||BC039580|
|Gene ID (NCBI)||83666|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Poly(ADP-ribosyl)ation is a post-translational modification of proteins mediated by one of the 17 members of the poly(ADP-ribose) polymerases (PARP). PARP-9 belongs to the subfamily of macroPARPs, associating one to three macro domains to the PARP domain. Overexpression of PARP-9 stimulates cell migration in vitro, suggesting a role for PARP-9 in the promotion of malignant B cell migration and dissemination in high risk DLBCL. PARP-9 is also likely a transcription coactivator, its overexpression in B lymphocytes, stimulated by interferon gamma (IFNγ), inducing the transcription of IFNγ-controlled genes.
PARP9 is overexpressed in human breast cancer and promotes cancer cell migration.
Exosomes Serve as Nanoparticles to Deliver Anti-miR-214 to Reverse Chemoresistance to Cisplatin in Gastric Cancer.
Ubiquitin Modification by the E3 Ligase/ADP-Ribosyltransferase Dtx3L/Parp9.