WB result of α-Parvin/Actopaxin antibody (55268-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-α-Parvin/Actopaxin transfected HeLa cells.
WB result of α-Parvin/Actopaxin antibody (55268-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-α-Parvin/Actopaxin transfected HeLa cells.
WB analysis of U2OS using 55268-1-AP
U2OS cells were subjected to SDS PAGE followed by western blot with 55268-1-AP (PARVA antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
U2OS cells were subjected to SDS PAGE followed by western blot with 55268-1-AP (PARVA antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of mouse kidney using 55268-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 55268-1-AP (PARVA antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 55268-1-AP (PARVA antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IP experiment of U2OS using 55268-1-AP
IP result of anti-Alpha Parvin/Actopaxin (IP:55268-1-AP, 4ug; Detection:55268-1-AP 1:1000) with U2OS cells lysate 0 ug.
IP result of anti-Alpha Parvin/Actopaxin (IP:55268-1-AP, 4ug; Detection:55268-1-AP 1:1000) with U2OS cells lysate 0 ug.
IHC staining of human stomach cancer using 55268-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer slide using 55268-1-AP (Alpha Parvin/Actopaxin antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human stomach cancer slide using 55268-1-AP (Alpha Parvin/Actopaxin antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 55268-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 55268-1-AP (PARVA Antibody) at dilution of 1:200 (under 10x lens).
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 55268-1-AP (PARVA Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human liver cancer using 55268-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 55268-1-AP (PARVA Antibody) at dilution of 1:200 (under 40x lens).
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 55268-1-AP (PARVA Antibody) at dilution of 1:200 (under 40x lens).
IHC staining of human heart using 55268-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 55268-1-AP (PARVA antibody) at dilution of 1:50 (under 10x lens).
Immunohistochemical analysis of paraffin-embedded human heart using 55268-1-AP (PARVA antibody) at dilution of 1:50 (under 40x lens).
IF Staining of MDCK using 55268-1-AP
Immunofluorescent analysis of MDCK cells using 55268-1-AP (PARVA antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunofluorescent analysis of MDCK cells using 55268-1-AP (PARVA antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
human stomach cancer tissue, human heart tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
MDCK cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:200-1:1000
Immunoprecipitation (IP)
IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)
IHC : 1:50-1:500
Immunofluorescence (IF)/ICC
IF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
α-Parvin (PARVA), also named as MXRA2, Actopaxin and CH-ILKBP, belongs to the parvin family. It probably plays a role in the regulation of cell adhesion and cytoskeleton organization. (PMID:11134073) This antibody is specific to α-Parvin.
Protocols
Product Specific Protocols
WB protocol for Alpha Parvin/Actopaxin antibody 55268-1-AP
WB result of α-Parvin/Actopaxin antibody (55268-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-α-Parvin/Actopaxin transfected HeLa cells.
WB analysis of U2OS using 55268-1-AP
U2OS cells were subjected to SDS PAGE followed by western blot with 55268-1-AP (PARVA antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of mouse kidney using 55268-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 55268-1-AP (PARVA antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human stomach cancer using 55268-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer slide using 55268-1-AP (Alpha Parvin/Actopaxin antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 55268-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 55268-1-AP (PARVA Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human liver cancer using 55268-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 55268-1-AP (PARVA Antibody) at dilution of 1:200 (under 40x lens).
IHC staining of human heart using 55268-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 55268-1-AP (PARVA antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human heart using 55268-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 55268-1-AP (PARVA antibody) at dilution of 1:50 (under 40x lens).
IP Figures
IP experiment of U2OS using 55268-1-AP
IP result of anti-Alpha Parvin/Actopaxin (IP:55268-1-AP, 4ug; Detection:55268-1-AP 1:1000) with U2OS cells lysate 0 ug.
IF/ICC Figures
IF Staining of MDCK using 55268-1-AP
Immunofluorescent analysis of MDCK cells using 55268-1-AP (PARVA antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
KD/KO VALIDATED
Alpha Parvin/Actopaxin Polyclonal antibody
Catalog Number
55268-1-AP
Citations
2
Dilutions
WB : 1:200-1:1000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:50-1:500 IF/ICC : 1:10-1:100
Applications
WB, IHC, IF/ICC, IP, ELISA
Reactivity
human, mouse, rat, canine
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
