|Positive WB detected in||mouse skin tissue, A375 cells, HepG2 cells, K-562 cells|
|Western Blot (WB)||WB : 1:200-1:1000|
|Sample-dependent, check data in validation data gallery|
51036-2-AP targets PAX3 in WB applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||PAX3 fusion protein Ag0477|
|Full Name||paired box 3|
|Calculated molecular weight||120 kDa|
|Observed molecular weight||56 kDa|
|GenBank accession number||BC114363|
|Gene ID (NCBI)||5077|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Aliquoting is unnecessary for -20oC storage.|
PAX3, a transcription factor and multifunctional regulatory protein, is normally expressed during embryonic development. In the nervous system, PAX3 is involved in neural tube closure, neural crest development, and peripheral neuron differentiation. In the present study, PAX3 was reported as a novel regulator of GFAP transcription, and the overexpression and suppression of PAX3 could inhibit and promote NSC differentiation, respectively. In muscle development, PAX3 ensures the survival of myogenic progenitor cells with Pax3-expressing progenitors giving rise to both skeletal and smooth muscle cells. PAX3 also has a well-established role in the development of melanocytes during embryogenesis, and has recently been characterized as a molecular switch in the mature melanocyte. Mutations in PAX3 can cause Waardenburg syndrome.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Kim (Verified Customer) (12-03-2019)
Tissue fixed in neutral buffered formalin, 24h, 4degC. Paraffin embedded. Citrate pH6.0/0.05% Tween antigen retrieval, 20 min ~90degC. Polymer-HRP secondary. No counterstain. DAB detection.Photos are: snout/tongue and back.