PAX3 Polyclonal antibody

PAX3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Reviews (1)

Cat no : 51036-2-AP

Synonyms

CDHS, HUP2, paired box 3, Paired box protein Pax 3, PAX3, WS1

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Validation Data Gallery

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  WB analysis of mouse skin using 51036-2-AP

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 51036-2-AP (PAX3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 51036-2-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 51036-2-AP (PAX3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 51036-2-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 51036-2-AP (PAX3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 51036-2-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 51036-2-AP (PAX3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of rat brain using 51036-2-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 51036-2-AP (PAX3 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 51036-2-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 51036-2-AP (PAX3 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse skin tissue, A375 cells, HepG2 cells, K-562 cells
• Positive IHC detected in: rat brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

51036-2-AP targets PAX3 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PAX3 fusion protein Ag0477
• Full Name: paired box 3
• Calculated Molecular Weight: 120 kDa
• Observed Molecular Weight: 56 kDa
• GenBank Accession Number: BC114363
• Gene Symbol: PAX3
• Gene ID (NCBI): 5077
• RRID: AB_670479
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PAX3, a transcription factor and multifunctional regulatory protein, is normally expressed during embryonic development. In the nervous system, PAX3 is involved in neural tube closure, neural crest development, and peripheral neuron differentiation. In the present study, PAX3 was reported as a novel regulator of GFAP transcription, and the overexpression and suppression of PAX3 could inhibit and promote NSC differentiation, respectively. In muscle development, PAX3 ensures the survival of myogenic progenitor cells with Pax3-expressing progenitors giving rise to both skeletal and smooth muscle cells. PAX3 also has a well-established role in the development of melanocytes during embryogenesis, and has recently been characterized as a molecular switch in the mature melanocyte. Mutations in PAX3 can cause Waardenburg syndrome.

Publications

Species	Application	Title
mouse	WB	Food Funct; N-3 polyunsaturated fatty acids effectively protect against neural tube defects in diabetic mice induced by streptozotocin.; Authors - Kelei Li
mouse	WB	J Agric Food Chem; Synergistic Effects of Folic Acid and n-3 Polyunsaturated Fatty Acid in Preventing Neural Tube Defects in Diabetic Mice; Authors - Kelei Li

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Kim (Verified Customer) (12-03-2019)

Tissue fixed in neutral buffered formalin, 24h, 4degC. Paraffin embedded. Citrate pH6.0/0.05% Tween antigen retrieval, 20 min ~90degC. Polymer-HRP secondary. No counterstain. DAB detection.Photos are: snout/tongue and back.

• Applications: Immunohistochemistry,
• Primary Antibody Dilution: 1/200
• Cell Tissue Type: E12.5 mouse embryo, sagittal section

PAX3 Antibody Immunohistochemistry, validation (1/200 dilution) in E12.5 mouse embryo, sagittal section (Cat no:51036-2-AP)