PAX3 Polyclonal antibody

PAX3 Polyclonal Antibody for WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB,ELISA

Conjugate

Unconjugated

Cat no : 51036-2-AP

Synonyms

CDHS, HUP2, paired box 3, Paired box protein Pax 3, PAX3, WS1



Tested Applications

Positive WB detected inmouse skin tissue, A375 cells, HepG2 cells, K-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Sample-dependent, check data in validation data gallery

Product Information

51036-2-AP targets PAX3 in WB,ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PAX3 fusion protein Ag0477
Full Name paired box 3
Calculated molecular weight 120 kDa
Observed molecular weight 56 kDa
GenBank accession numberBC114363
Gene symbol PAX3
Gene ID (NCBI) 5077
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

PAX3, a transcription factor and multifunctional regulatory protein, is normally expressed during embryonic development. In the nervous system, PAX3 is involved in neural tube closure, neural crest development, and peripheral neuron differentiation. In the present study, PAX3 was reported as a novel regulator of GFAP transcription, and the overexpression and suppression of PAX3 could inhibit and promote NSC differentiation, respectively. In muscle development, PAX3 ensures the survival of myogenic progenitor cells with Pax3-expressing progenitors giving rise to both skeletal and smooth muscle cells. PAX3 also has a well-established role in the development of melanocytes during embryogenesis, and has recently been characterized as a molecular switch in the mature melanocyte. Mutations in PAX3 can cause Waardenburg syndrome.

Protocols

Product Specific Protocols
WB protocol for PAX3 antibody 51036-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Kim (Verified Customer) (12-03-2019)

Tissue fixed in neutral buffered formalin, 24h, 4degC. Paraffin embedded. Citrate pH6.0/0.05% Tween antigen retrieval, 20 min ~90degC. Polymer-HRP secondary. No counterstain. DAB detection.Photos are: snout/tongue and back.

  • Applications: Immunohistochemistry,
  • Primary Antibody Dilution: 1/200
  • Cell Tissue Type: E12.5 mouse embryo, sagittal section
PAX3 Antibody Immunohistochemistry, validation (1/200 dilution) in E12.5 mouse embryo, sagittal section (Cat no:51036-2-AP)