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  • KD/KO Validated

PAX6 Polyclonal antibody

PAX6 Polyclonal Antibody for FC, IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, FC,ELISA

Conjugate

Unconjugated

Cat no : 12323-1-AP

Synonyms

AN, AN2, Aniridia type II protein, D11S812E, MGDA, Oculorhombin, paired box 6, Paired box protein Pax 6, PAX6, WAGR



Tested Applications

Positive WB detected inY79 cells, human stomach tissue, mouse embryo tissue, rat brain tissue
Positive IHC detected inmouse brain tissue, mouse embryo tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse brain tissue, iPS cells, Neuro-2a cells
Positive FC detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

12323-1-AP targets PAX6 in WB, IHC, IF, FC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PAX6 fusion protein Ag2984
Full Name paired box 6
Calculated molecular weight 47 kDa
Observed molecular weight 47 kDa
GenBank accession numberBC011953
Gene symbol PAX6
Gene ID (NCBI) 5080
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Background

Paired-box 6 (Pax6) is a highly conserved nuclear transcription factor that is key in neural tissue development, particularly eye morphogenesis.

What is the molecular weight of Pax6?

46.6 kDa. Pax6 is composed of 422 amino acids and contains a paired domain and a homeodomain.

What is the role of Pax6 in development?

In embryos, Pax6 is expressed in the developing central nervous system (CNS), eye, nose, and also in the pancreas. As a transcription factor, Pax6 is one of the earliest genes expressed in the eye and regulates the expression of many genes required for retinal and olfactory development (PMID: 10461206). The broad expression of Pax6 in the neuroectoderm, which develops to the CNS, makes it a useful marker for neural stem cells (PMID: 20621053).

The homology of Pax6 to the Drosophila gene eyeless and the mouse gene Small eye highlights its importance across a wide spectrum of organisms. In model organisms such as flies or mice, a dramatic phenotype can be seen. Mutations in eyeless lead to ectopic eye development and mice with heterozygote small eye mutations do not develop eyes or nasal cavities, and homozygote small eye mice do not survive (PMID: 7892602; PMID 8875254).

What is the role of Pax6 in eye disease?

Due to its key role as a master regulator in eye development, mutations in Pax6 have been associated with many different eye diseases. In humans, premature stop signals that lead to the production of a truncated protein have been found to cause aniridia, the absence of an iris (PMID: 19898691). Single amino acid mutations in Pax6 may lead to Peters' anomaly, which causes thinning and clouding of the cornea (PMID: 8162071).


Protocols

Product Specific Protocols
WB protocol for PAX6 antibody 12323-1-APDownload protocol
IHC protocol for PAX6 antibody 12323-1-APDownload protocol
IF protocol for PAX6 antibody 12323-1-APDownload protocol
FC protocol for PAX6 antibody 12323-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

ACS Nano

Silver Nanoparticles Compromise Female Embryonic Stem Cell Differentiation through Disturbing X Chromosome Inactivation.

Authors - Jie Zhang
humanIF

Stem Cells Dev

Rapid Differentiation of Multi-Zone Ocular Cells from Human Induced Pluripotent Stem Cells and Generation of Corneal Epithelial and Endothelial Cells.

Authors - Zongyi Li
humanWB,IHC,IF

Cell Death Dis

PMID: 31024010

Authors - Dong-Ming Wu
  • KD Validated
IF

Cell Stem Cell

Engineering Genetic Predisposition in Human Neuroepithelial Stem Cells Recapitulates Medulloblastoma Tumorigenesis.

Authors - Miller Huang
mouseWB

Cell Metab

Mitochondrial Dynamics Is Critical for the Full Pluripotency and Embryonic Developmental Potential of Pluripotent Stem Cells.

Authors - Xiuying Zhong
humanIF

Am J Stem Cells

A robust method to derive functional neural crest cells from human pluripotent stem cells.

Authors - Kreitzer Faith R FR

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Julie (Verified Customer) (01-18-2021)

This antibody works very well for immunofluorescence on human brain.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: Human
FH

Rachel (Verified Customer) (02-01-2019)

Worked well and Proteintech employees were very helpful during the ordering process.

  • Applications: Other,
  • Cell Tissue Type: Epithelial cells