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PAX6 Polyclonal antibody

PAX6 Polyclonal Antibody for WB, IF, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat and More (1)





Cat no : 12323-1-AP


AN, AN2, Aniridia type II protein, D11S812E, MGDA, Oculorhombin, paired box 6, Paired box protein Pax 6, PAX6, WAGR

Tested Applications

Positive WB detected inY79 cells, mouse embryo tissue, human stomach tissue, rat brain tissue
Positive IHC detected inmouse brain tissue, mouse eye tissue, human retinoblastoma tissue, mouse embryo tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inRetinal organoids, iPS cells
Positive FC detected inSH-SY5Y cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:10-1:100
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12323-1-AP targets PAX6 in WB, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, monkey
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PAX6 fusion protein Ag2984
Full Name paired box 6
Calculated Molecular Weight 47 kDa
Observed Molecular Weight 47 kDa
GenBank Accession NumberBC011953
Gene Symbol PAX6
Gene ID (NCBI) 5080
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information


Paired-box 6 (Pax6) is a highly conserved nuclear transcription factor that is key in neural tissue development, particularly eye morphogenesis.

What is the molecular weight of Pax6?

46.6 kDa. Pax6 is composed of 422 amino acids and contains a paired domain and a homeodomain.

What is the role of Pax6 in development?

In embryos, Pax6 is expressed in the developing central nervous system (CNS), eye, nose, and also in the pancreas. As a transcription factor, Pax6 is one of the earliest genes expressed in the eye and regulates the expression of many genes required for retinal and olfactory development (PMID: 10461206). The broad expression of Pax6 in the neuroectoderm, which develops to the CNS, makes it a useful marker for neural stem cells (PMID: 20621053).

The homology of Pax6 to the Drosophila gene eyeless and the mouse gene Small eye highlights its importance across a wide spectrum of organisms. In model organisms such as flies or mice, a dramatic phenotype can be seen. Mutations in eyeless lead to ectopic eye development and mice with heterozygote small eye mutations do not develop eyes or nasal cavities, and homozygote small eye mice do not survive (PMID: 7892602; PMID 8875254).

What is the role of Pax6 in eye disease?

Due to its key role as a master regulator in eye development, mutations in Pax6 have been associated with many different eye diseases. In humans, premature stop signals that lead to the production of a truncated protein have been found to cause aniridia, the absence of an iris (PMID: 19898691). Single amino acid mutations in Pax6 may lead to Peters' anomaly, which causes thinning and clouding of the cornea (PMID: 8162071).


Product Specific Protocols
WB protocol for PAX6 antibody 12323-1-APDownload protocol
IHC protocol for PAX6 antibody 12323-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Ryszard (Verified Customer) (01-19-2024)

Antibody Pax-6 Rabbit works very well on whole mount mESC-derived cerebral organoids.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1/500
  • Cell Tissue Type: mESC-derived Cerebral Organoids
PAX6 Antibody Immunofluorescence validation (1/500 dilution) in mESC-derived Cerebral Organoids (Cat no:12323-1-AP)

Julie (Verified Customer) (01-18-2021)

This antibody works very well for immunofluorescence on human brain.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: Human

Rachel (Verified Customer) (02-01-2019)

Worked well and Proteintech employees were very helpful during the ordering process.

  • Applications: Other,
  • Cell Tissue Type: Epithelial cells