PAX7 Polyclonal antibody

PAX7 Polyclonal Antibody for IF, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 20570-1-AP

Synonyms

HUP1, paired box 7, Paired box protein Pax 7, PAX7, PAX7B



Tested Applications

Positive WB detected inNCCIT cell, Jurkat cells, HeLa cells, C2C12 cell
Positive IF detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

20570-1-AP targets PAX7 in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name paired box 7
Calculated molecular weight 57 kDa
Observed molecular weight 57 kDa
GenBank accession numberNM_002584
Gene symbol PAX7
Gene ID (NCBI) 5081
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Pax7 is a highly conserved transcription factor involved in early development of the neural crest and gastrulation and is a commonly used marker for satellite cells in muscle tissue. This antibody is specific to PAX7. It will not cross-react with other PAX protein family members.

What is the molecular weight of Pax7?

57 kDa

What is the role of Pax7?

One of the Paired Box (Pax) transcription factors, which are key in early development and organogenesis in vertebrates, Pax7 binds directly to DNA and is key in neural crest induction (PMID: 26410165). One of the earlier markers in this phase of embryogenesis, Pax7 therefore influences the migration and proliferation of multiple lineages of cells (PMID: 22848431).

In adult tissue, Pax7 is expressed by the quiescent resident muscle stem cells, also known as satellite cells, making it a useful marker for this cell type. It is essential for their formation and maintenance in their niche. When activated, these cells can proliferate in the normal maintenance of muscle tissue or respond to injury in repair. The co-expression of Pax7 with MyoD in these activated satellite cells regulates muscle cell proliferation (PMID: 16608873).

What proteins does Pax7 interact with?

An orthologue of Pax7, another transcription factor of the same family, is Pax3. These two proteins can form a heterodimer, binding to DNA to work together in myogenesis but can also function independently and have overlapping roles (PMID: 16380438).

What is the role of Pax7 in disease?

Pax7 and Pax 3 have both been identified in tumor tissue from the childhood cancer alveolar rhabdomyosarcoma (ARMS), which affects skeletal muscle (PMID: 23206814).


Protocols

Product Specific Protocols
WB protocol for PAX7 antibody 20570-1-APDownload protocol
IF protocol for PAX7 antibody 20570-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Skelet Muscle

Dilated cardiomyopathy-mediated heart failure induces a unique skeletal muscle myopathy with inflammation.

Authors - Taejeong Song
mouseWB

Exp Physiol

Overexpression of Dnmt3a ameliorates diabetic muscle atrophy by modulating Pten/Akt pathway.

Authors - Manfeng Wang
mouseIF

Biochem Biophys Res Commun

Exosomes of oral squamous cell carcinoma cells containing miR-181a-3p induce muscle cell atrophy and apoptosis by transmissible endoplasmic reticulum stress signaling.

Authors - Ling Qiu
mouseIHC

Sci Rep

Anti-aging Effect of Transplanted Amniotic Membrane Mesenchymal Stem Cells in a Premature Aging Model of Bmi-1 Deficiency.

Authors - Chunfeng Xie
mouseWB

Front Cell Dev Biol

PERK Signaling Controls Myoblast Differentiation by Regulating MicroRNA Networks.

Authors - Ye-Ya Tan
mouseIF, WB

Theranostics

Trio cooperates with Myh9 to regulate neural crest-derived craniofacial development.

Authors - Shuyu Guo

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Sahya (Verified Customer) (08-13-2018)

Protocol - Primary Antibody - 1 hourPBS washSecondary Antibody - 1 hour

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:100 and 1:200
  • Cell Tissue Type: Skeletal Muscle Satellite Cells