PAX8 Monoclonal antibody

PAX8 Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG2b


human, mouse , rat







Cat no : 60145-4-Ig


paired box 8, Paired box protein Pax 8, PAX8

Tested Applications

Positive WB detected inSKOV-3 cells, HepG2 cells, human thyroid gland tissue
Positive IHC detected inhuman ovary tumor tissue, human tonsillitis tissue, human thyroid cancer tissue, rat kidney tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inSKOV-3 cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:5000-1:20000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60145-4-Ig targets PAX8 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse , rat samples.

Tested Reactivity human, mouse , rat
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen PAX8 fusion protein Ag0306
Full Name paired box 8
Calculated Molecular Weight 48 kDa
Observed Molecular Weight 48 kDa
GenBank Accession NumberBC001060
Gene Symbol PAX8
Gene ID (NCBI) 7849
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PAX8 is a member of the paired box (PAX) family of transcription factors, typically containing a paired box domain and a paired-type homeodomain. It is expressed during organogenesis of the thyroid gland, kidney and Mullerian system. It is thought to regulate the expression of Wilms tumor suppressor (WT1) gene and mutations in PAX8 have been associated with Wilms tumor cells, thyroid and ovarian carcinomas. PAX8 is a useful marker in distinguishing ovarian carcinomas from mammary carcinomas (PMID: 18724243). PAX8 is expressed in a high percentage of ovarian serous, endometrioid, and clear cell carcinomas, but only rarely in primary ovarian mucinous adenocarcinomas.

PAX8 has 5 isoforms with calculated MW 31kd, 34kd, 41kd, 43kd and 48kd. And there is a 60kd band corresponding to the electrophoretic mobility of PAX8 (PMID:15650356).


Product Specific Protocols
WB protocol for PAX8 antibody 60145-4-IgDownload protocol
IHC protocol for PAX8 antibody 60145-4-IgDownload protocol
IF protocol for PAX8 antibody 60145-4-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Dev Cell

Identification of a core transcriptional program driving the human renal mesenchymal-to-epithelial transition

Authors - John-Poul Ng-Blichfeldt

Mod Pathol

Xp11.2 translocation renal cell carcinoma with NONO-TFE3 gene fusion: morphology, prognosis, and potential pitfall in detecting TFE3 gene rearrangement.

Authors - Qiu-Yuan Xia

Stem Cell Res Ther

Genetic correction of induced pluripotent stem cells from a DFNA36 patient results in morphologic and functional recovery of derived hair cell-like cells

Authors - Yi Luo

Front Cell Dev Biol

Lgr4 Regulates Oviductal Epithelial Secretion Through the WNT Signaling Pathway

Authors - Xue Tan

Sci Rep

Differential epithelial and stromal LGR5 expression in ovarian carcinogenesis.

Authors - Hyesung Kim

Hum Pathol

Clinicopathological and Molecular Characterization of Biphasic Hyalinizing Psammomatous Renal Cell Carcinoma (BHP RCC): Further Support for the Newly Proposed Entity.

Authors - Xiao-Tong Wang


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Kenzo (Verified Customer) (03-28-2024)

It worked well for immunolabeling in mouse retina tissues

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Mouse retina

Juan (Verified Customer) (04-18-2019)

It works well in Western Blot experiment.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HepG2 Cell line