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Pyruvate Carboxylase Polyclonal antibody
Pyruvate Carboxylase Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Cat no : 16588-1-AP
Validation Data Gallery
|Positive WB detected in
|mouse liver tissue, HepG2 cells, rat liver tissue
|Positive IP detected in
|HepG2 cells, mouse liver tissue
|Positive IHC detected in
|human liver cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|Western Blot (WB)
|WB : 1:5000-1:50000
|IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
|IHC : 1:500-1:2000
|IF : 1:50-1:500
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
16588-1-AP targets Pyruvate Carboxylase in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|human, mouse, rat
|human, mouse, rat, pig, bovine
|Host / Isotype
|Rabbit / IgG
|Pyruvate Carboxylase fusion protein Ag8857
|Calculated molecular weight
|1178 aa, 130 kDa
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
PC(pyruvate carboxylase) is a member of the family of biotin-dependent carboxylases and is found widely among eukaryotic tissues and in many prokaryotic species. It catalyses the ATP-dependent carboxylation of pyruvate to form oxaloacetate which may be utilised in the synthesis of glucose, fat, some amino acids or their derivatives and several neurotransmitters. Diabetes and hyperthyroidism increase the level of expression of pyruvate carboxylase in the long term, while its activity in the short term is controlled by the intramitochondrial concentrations of acetyl-CoA and pyruvate(PMID:9597748).
|Product Specific Protocols
|WB protocol for Pyruvate Carboxylase antibody 16588-1-AP
|IHC protocol for Pyruvate Carboxylase antibody 16588-1-AP
|IF protocol for Pyruvate Carboxylase antibody 16588-1-AP
|IP protocol for Pyruvate Carboxylase antibody 16588-1-AP
|Click here to view our Standard Protocols
Pyruvate anaplerosis is a targetable vulnerability in persistent leukaemic stem cells
Enhancement of anaerobic glycolysis - a role of PGC-1α4 in resistance exercise.
The long noncoding RNA ADIPINT regulates human adipocyte metabolism via pyruvate carboxylase.
SARS-CoV-2 infection rewires host cell metabolism and is potentially susceptible to mTORC1 inhibition.
A short isoform of spermatogenic enzyme GAPDHS functions as a metabolic switch and limits metastasis in melanoma.
Oxid Med Cell Longev
LINC00092 Modulates Oxidative Stress and Glycolysis of Breast Cancer Cells via Pyruvate Carboxylase-Mediated AKT/mTOR Pathway.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Kevin (Verified Customer) (02-10-2022)
This worked as well as other much more expensive antibodies from differenet suppliers