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Pyruvate Carboxylase Polyclonal antibody

Pyruvate Carboxylase Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat and More (1)





Cat no : 16588-1-AP


PC, PCB, pyruvate carboxykinase, pyruvate Carboxylase, Pyruvic carboxylase

Tested Applications

Positive WB detected inmouse liver tissue, HepG2 cells, rat liver tissue
Positive IP detected inHepG2 cells, mouse liver tissue
Positive IHC detected inhuman liver cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 10 publications below

Product Information

16588-1-AP targets Pyruvate Carboxylase in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, pig, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Pyruvate Carboxylase fusion protein Ag8857
Full Name pyruvate carboxylase
Calculated molecular weight 1178 aa, 130 kDa
Observed molecular weight 125-130 kDa
GenBank accession numberBC011617
Gene symbol PC
Gene ID (NCBI) 5091
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PC(pyruvate carboxylase) is a member of the family of biotin-dependent carboxylases and is found widely among eukaryotic tissues and in many prokaryotic species. It catalyses the ATP-dependent carboxylation of pyruvate to form oxaloacetate which may be utilised in the synthesis of glucose, fat, some amino acids or their derivatives and several neurotransmitters. Diabetes and hyperthyroidism increase the level of expression of pyruvate carboxylase in the long term, while its activity in the short term is controlled by the intramitochondrial concentrations of acetyl-CoA and pyruvate(PMID:9597748).


Product Specific Protocols
WB protocol for Pyruvate Carboxylase antibody 16588-1-APDownload protocol
IHC protocol for Pyruvate Carboxylase antibody 16588-1-APDownload protocol
IF protocol for Pyruvate Carboxylase antibody 16588-1-APDownload protocol
IP protocol for Pyruvate Carboxylase antibody 16588-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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