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Pyruvate Carboxylase Polyclonal antibody
Pyruvate Carboxylase Polyclonal Antibody for IF, IHC, IP, WB,ELISA
Cat no : 16588-1-AP
Validation Data Gallery
|Positive WB detected in||mouse liver tissue, HepG2 cells, rat liver tissue|
|Positive IP detected in||HepG2 cells, mouse liver tissue|
|Positive IHC detected in||human liver cancer tissue, human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:500-1:2000|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
16588-1-AP targets Pyruvate Carboxylase in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, bovine, pig|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Pyruvate Carboxylase fusion protein Ag8857|
|Full Name||pyruvate carboxylase|
|Calculated molecular weight||1178 aa, 130 kDa|
|Observed molecular weight||125-130 kDa|
|GenBank accession number||BC011617|
|Gene ID (NCBI)||5091|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
PC(pyruvate carboxylase) is a member of the family of biotin-dependent carboxylases and is found widely among eukaryotic tissues and in many prokaryotic species. It catalyses the ATP-dependent carboxylation of pyruvate to form oxaloacetate which may be utilised in the synthesis of glucose, fat, some amino acids or their derivatives and several neurotransmitters. Diabetes and hyperthyroidism increase the level of expression of pyruvate carboxylase in the long term, while its activity in the short term is controlled by the intramitochondrial concentrations of acetyl-CoA and pyruvate(PMID:9597748).
|Product Specific Protocols|
|WB protocol for Pyruvate Carboxylase antibody 16588-1-AP||Download protocol|
|IHC protocol for Pyruvate Carboxylase antibody 16588-1-AP||Download protocol|
|IF protocol for Pyruvate Carboxylase antibody 16588-1-AP||Download protocol|
|IP protocol for Pyruvate Carboxylase antibody 16588-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Kevin (Verified Customer) (02-10-2022)
This worked as well as other much more expensive antibodies from differenet suppliers