PCARE Polyclonal antibody

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  WB analysis using 31187-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31187-1-AP (PCARE antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

PCARE Polyclonal Antibody for WB, ELISA

Cat No. 31187-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Synonyms

C2orf71, FLJ34931

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Tested Applications

• Positive WB detected in: mouse eye tissue, mouse retina tissue, rat eye tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31187-1-AP targets PCARE in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PCARE fusion protein Ag34774
• Full Name: chromosome 2 open reading frame 71
• Calculated Molecular Weight: 140 kDa
• Observed Molecular Weight: 140 kDa
• GenBank Accession Number: NM_001029883
• Gene Symbol: PCARE
• Gene ID (NCBI): 388939
• RRID: AB_3669889
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: A6NGG8
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

C2orf71/PCARE (photoreceptor cilium actin regulator) is a retina-specific protein consisting of 1289 amino acids with a predicted molecular weight of 140 kDa. PCARE is predicted to be myristoylated and palmitoylated at its N terminus and plays an important role in delivering actin-associated components to the base of the photoreceptor OSs to regulate the initial development of OS disks (PMID: 32312818, 35253837).