|Positive WB detected in||mouse kidney tissue, A431 cells, HEK-293 cells, HeLa cells, human saliva tissue, mouse heart tissue, mouse lung tissue, PC-3 cells, rat brain tissue, THP-1 cells, U-937 cells|
|Positive IP detected in||mouse kidney tissue|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Sample-dependent, check data in validation data gallery|
18410-1-AP targets Granulin in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Granulin fusion protein Ag13178|
|Calculated molecular weight||64 kDa|
|Observed molecular weight||68 kDa, 70-88 kDa|
|GenBank accession number||BC000324|
|Gene ID (NCBI)||2896|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
GRN, also known as PGRN or PCDGF, is a cysteine-rich protein of 68.5 kDa that is typically secreted into a highly glycosylated 88 kDa form. PGRN is a unique growth factor that plays an important role in cutaneous wound healing. It has an anti-inflammatory effect and promotes cell proliferation. When PCDGF is degraded to several 6-25 kDa fragments, called granulins (GRNs) by neutrophil proteases, a pro-inflammatory reaction occurs. PGRN is widely expressed, particularly in epithelial cells, immune cells, neurons, and chondrocytes. High levels of PGRN expression have been reported in human cancers, and its expression is closely correlated with the development and metastasis of several cancers. The recent discovery that mutations in the gene encoding for pro-granulin (GRN) cause frontotemporal lobar degeneration (FTLD), and other neurodegenerative diseases leading to dementia, has brought renewed interest in progranulin and its functions in the central nervous system.
Mol Med Rep
A method for establishing human primary gastric epithelial cell culture from fresh surgical gastric tissues.
Clin Exp Dermatol
Significance of PC cell-derived growth factor and cyclin D1 expression in cutaneous squamous cell carcinoma.
J Cancer Res Clin Oncol
Progranulin is preferentially expressed in patients with psoriasis vulgaris and protects mice from psoriasis-like skin inflammation.
Int J Mol Sci
Involvement of Progranulin and Granulin Expression in Inflammatory Responses after Cerebral Ischemia.
J Mol Med (Berl)
Progranulin alleviates podocyte injury via regulating CAMKK/AMPK-mediated autophagy under diabetic conditions.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Sharan (Verified Customer) (06-24-2020)
The antibody detects the GRN protein but there is also a lot of cross-reactivity with non-specific antigens in HeLa lysate