- Featured Product
- KD/KO Validated
Granulin Polyclonal antibody
Granulin Polyclonal Antibody for IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 18410-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | mouse kidney tissue, human saliva, A431 cells, HeLa cells, rat brain tissue, HEK-293 cells, PC-3 cells, mouse heart tissue, mouse lung tissue, U-937 cells, THP-1 cells, rat heart tissue, rat kidney tissue |
Positive IP detected in | mouse kidney tissue |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 1 publications below |
WB | See 4 publications below |
IHC | See 5 publications below |
IF | See 2 publications below |
Product Information
18410-1-AP targets Granulin in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Granulin fusion protein Ag13178 |
Full Name | granulin |
Calculated molecular weight | 64 kDa |
Observed molecular weight | 68 kDa, 70-88 kDa |
GenBank accession number | BC000324 |
Gene symbol | GRN |
Gene ID (NCBI) | 2896 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
GRN, also known as PGRN or PCDGF, is a cysteine-rich protein of 68.5 kDa that is typically secreted into a highly glycosylated 88 kDa form. PGRN is a unique growth factor that plays an important role in cutaneous wound healing. It has an anti-inflammatory effect and promotes cell proliferation. When PCDGF is degraded to several 6-25 kDa fragments, called granulins (GRNs) by neutrophil proteases, a pro-inflammatory reaction occurs. PGRN is widely expressed, particularly in epithelial cells, immune cells, neurons, and chondrocytes. High levels of PGRN expression have been reported in human cancers, and its expression is closely correlated with the development and metastasis of several cancers. The recent discovery that mutations in the gene encoding for pro-granulin (GRN) cause frontotemporal lobar degeneration (FTLD), and other neurodegenerative diseases leading to dementia, has brought renewed interest in progranulin and its functions in the central nervous system.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for Granulin antibody 18410-1-AP | Download protocol |
IP protocol for Granulin antibody 18410-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Mol Med Rep A method for establishing human primary gastric epithelial cell culture from fresh surgical gastric tissues. | ||
Clin Exp Dermatol Significance of PC cell-derived growth factor and cyclin D1 expression in cutaneous squamous cell carcinoma. | ||
Immunology Progranulin is preferentially expressed in patients with psoriasis vulgaris and protects mice from psoriasis-like skin inflammation.
| ||
Int J Mol Sci Involvement of Progranulin and Granulin Expression in Inflammatory Responses after Cerebral Ischemia. | ||
Neuropharmacology Abnormal spatiotemporal expression pattern of progranulin and neurodevelopment impairment in VPA-induced ASD rat model. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Sharan (Verified Customer) (06-24-2020) | The antibody detects the GRN protein but there is also a lot of cross-reactivity with non-specific antigens in HeLa lysate
![]() |