• Featured Product
  • KD/KO Validated

Granulin Polyclonal antibody

Granulin Polyclonal Antibody for IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 18410-1-AP

Synonyms

acrogranin, GEP, GP88, granulin, Granulin A Granulin 5, Granulin B Granulin 4, Granulin C Granulin 6, Granulin D Granulin 7, Granulin E, Granulin F Granulin 3, Granulin G Granulin 2, Granulins, GRN, PCDGF, PEPI, PGRN, Proepithelin



Tested Applications

Positive WB detected inmouse kidney tissue, A431 cells, HEK-293 cells, HeLa cells, human saliva tissue, mouse heart tissue, mouse lung tissue, PC-3 cells, rat brain tissue, THP-1 cells, U-937 cells
Positive IP detected inmouse kidney tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Sample-dependent, check data in validation data gallery

Product Information

18410-1-AP targets Granulin in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Granulin fusion protein Ag13178
Full Name granulin
Calculated molecular weight 64 kDa
Observed molecular weight 68 kDa, 70-88 kDa
GenBank accession numberBC000324
Gene symbol GRN
Gene ID (NCBI) 2896
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GRN, also known as PGRN or PCDGF, is a cysteine-rich protein of 68.5 kDa that is typically secreted into a highly glycosylated 88 kDa form. PGRN is a unique growth factor that plays an important role in cutaneous wound healing. It has an anti-inflammatory effect and promotes cell proliferation. When PCDGF is degraded to several 6-25 kDa fragments, called granulins (GRNs) by neutrophil proteases, a pro-inflammatory reaction occurs. PGRN is widely expressed, particularly in epithelial cells, immune cells, neurons, and chondrocytes. High levels of PGRN expression have been reported in human cancers, and its expression is closely correlated with the development and metastasis of several cancers. The recent discovery that mutations in the gene encoding for pro-granulin (GRN) cause frontotemporal lobar degeneration (FTLD), and other neurodegenerative diseases leading to dementia, has brought renewed interest in progranulin and its functions in the central nervous system.

Protocols

Product Specific Protocols
WB protocol for Granulin antibody 18410-1-APDownload protocol
IP protocol for Granulin antibody 18410-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Mol Med Rep

A method for establishing human primary gastric epithelial cell culture from fresh surgical gastric tissues.

Authors - Faisal Aziz
humanIHC

Clin Exp Dermatol

Significance of PC cell-derived growth factor and cyclin D1 expression in cutaneous squamous cell carcinoma.

Authors - Huang K K
humanWB, IHC

J Cancer Res Clin Oncol

PMID: 25527419

Authors - Faisal Aziz
mouseIHC

Immunology

Progranulin is preferentially expressed in patients with psoriasis vulgaris and protects mice from psoriasis-like skin inflammation.

Authors - Kun Huang
  • KO Validated
ratWB,IHC,IF

Int J Mol Sci

Involvement of Progranulin and Granulin Expression in Inflammatory Responses after Cerebral Ischemia.

Authors - Ichiro Horinokita
ratWB

Neuropharmacology

Abnormal spatiotemporal expression pattern of progranulin and neurodevelopment impairment in VPA-induced ASD rat model.

Authors - Junying Lan

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Sharan (Verified Customer) (06-24-2020)

The antibody detects the GRN protein but there is also a lot of cross-reactivity with non-specific antigens in HeLa lysate

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HeLa cells
Granulin Antibody Western Blot, validation (1:1000 dilution) in HeLa cells (Cat no:18410-1-AP)