Name: Granulin Rabbit Polyclonal antibody
Brand: Proteintech
SKU: 18410-1-AP

Tested Applications

Applications:

WB, IP, IHC, IF, ELISA
Application not tested? Please see our Guarantee

Granulin Antibody WB mouse kidney tissue 18410-1-AP

Various lysates were subjected to SDS PAGE followed by western blot with 18410-1-AP (Granulin antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours

Granulin Antibody IP mouse kidney tissue 18410-1-AP

IP Result of anti-Granulin (IP:18410-1-AP, 4ug; Detection:18410-1-AP 1:800) with mouse kidney tissue lysate 4000ug.

Species specificity:

human, mouse, rat

Positive WB detected in:

mouse kidney tissue, A431 cells, HEK-293 cells, HeLa cells, human saliva tissue, mouse heart tissue, mouse lung tissue, PC-3 cells, rat brain tissue, THP-1 cells, U-937 cells

Positive IP detected in:

mouse kidney tissue

Recommended dilution:

WB : 1:500-1:1000

IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB

Sample-dependent, check data in Validation tab

Product Information

Source:

Rabbit

Purification method:

Antigen affinity purification

Isotype:

IgG

Storage:

PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Store at -20^oC. Aliquoting is unnecessary for -20^oC storage.

Immunogen Information

Immunogen:

Granulin fusion protein Ag13178

Full name:

granulin

Calculated molecular weight:

64 kDa

Observed molecular weight:

68 kDa, 70-88 kDa

GenBank accession number:

BC000324

Gene ID (NCBI):

2896

Gene symbol

GRN

Synonyms

acrogranin, GEP, GP88, granulin, Granulin A Granulin 5, Granulin B Granulin 4, Granulin C Granulin 6, Granulin D Granulin 7, Granulin E, Granulin F Granulin 3, Granulin G Granulin 2, Granulins, GRN, PCDGF, PEPI, PGRN, Proepithelin

Background

GRN, also known as PGRN or PCDGF, is a cysteine-rich protein of 68.5 kDa that is typically secreted into a highly glycosylated 88 kDa form. PGRN is a unique growth factor that plays an important role in cutaneous wound healing. It has an anti-inflammatory effect and promotes cell proliferation. When PCDGF is degraded to several 6–25 kDa fragments, called granulins (GRNs) by neutrophil proteases, a pro-inflammatory reaction occurs. PGRN is widely expressed, particularly in epithelial cells, immune cells, neurons, and chondrocytes. High levels of PGRN expression have been reported in human cancers, and its expression is closely correlated with the development and metastasis of several cancers. The recent discovery that mutations in the gene encoding for pro-granulin (GRN) cause frontotemporal lobar degeneration (FTLD), and other neurodegenerative diseases leading to dementia, has brought renewed interest in progranulin and its functions in the central nervous system.

Showing 1- [6] of [7] Publications

Author:		Kun Huang
Pubmed ID:		25626394
Journal:		Immunology
Title:		Progranulin is preferentially expressed in patients with psoriasis vulgaris and protects mice from psoriasis-like skin inflammation.
Application:		IHC
KO validated
Species:		mouse

Author:		Faisal Aziz
Pubmed ID:		25937205
Journal:		Mol Med Rep
Title:		A method for establishing human primary gastric epithelial cell culture from fresh surgical gastric tissues.
Application:

Species:

Author:		Di Zhou
Pubmed ID:		31402399
Journal:		J Mol Med (Berl)
Title:		Progranulin alleviates podocyte injury via regulating CAMKK/AMPK-mediated autophagy under diabetic conditions.
Application:		IF

Species:		human

Author:		Ichiro Horinokita
Pubmed ID:		31640144
Journal:		Int J Mol Sci
Title:		Involvement of Progranulin and Granulin Expression in Inflammatory Responses after Cerebral Ischemia.
Application:		WB,IHC,IF

Species:		rat

Author:		Loei Hendrick H
Pubmed ID:		22204653
Journal:		J Proteome Res
Title:		Mining the gastric cancer secretome: identification of GRN as a potential diagnostic marker for early gastric cancer.
Application:		IHC

Species:		human

Author:		Huang K K
Pubmed ID:		22420613
Journal:		Clin Exp Dermatol
Title:		Significance of PC cell-derived growth factor and cyclin D1 expression in cutaneous squamous cell carcinoma.
Application:		IHC

Species:		human

WB Figures

Granulin Antibody WB A431 cells 18410-1-AP

A431 cells were subjected to SDS PAGE followed by western blot with 18410-1-AP(Granulin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

Granulin Antibody WB HEK-293 cells 18410-1-AP

HEK-293 cells were subjected to SDS PAGE followed by western blot with 18410-1-AP(Granulin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

Granulin Antibody WB HeLa cells 18410-1-AP

HeLa cells were subjected to SDS PAGE followed by western blot with 18410-1-AP(Granulin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

Granulin Antibody WB human saliva tissue 18410-1-AP

human saliva were subjected to SDS PAGE followed by western blot with 18410-1-AP (Granulin antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours

Granulin Antibody WB mouse heart tissue 18410-1-AP

mouse heart tissue were subjected to SDS PAGE followed by western blot with 18410-1-AP(Granulin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

Various lysates were subjected to SDS PAGE followed by western blot with 18410-1-AP (Granulin antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours

Granulin Antibody WB mouse lung tissue 18410-1-AP

mouse lung tissue were subjected to SDS PAGE followed by western blot with 18410-1-AP(Granulin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

Granulin Antibody WB PC-3 cells 18410-1-AP

PC-3 cells were subjected to SDS PAGE followed by western blot with 18410-1-AP(Granulin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

Granulin Antibody WB rat brain tissue 18410-1-AP

rat brain tissue were subjected to SDS PAGE followed by western blot with 18410-1-AP(Granulin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

Granulin Antibody WB THP-1 cells 18410-1-AP

THP-1 cells were subjected to SDS PAGE followed by western blot with 18410-1-AP( Granulin antibody at dilution of 1:300 incubated at room temperature for 1.5 hours

Granulin Antibody WB U-937 cells 18410-1-AP

U-937 cells were subjected to SDS PAGE followed by western blot with 18410-1-AP(Granulin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

IHC Figures

Granulin Antibody IHC human kidney tissue 18410-1-AP

Immunohistochemical analysis of paraffin-embedded human kidney using 18410-1-AP(Granulin antibody) at dilution of 1:50 (under 10x lens)

Immunohistochemical analysis of paraffin-embedded human kidney using 18410-1-AP(Granulin antibody) at dilution of 1:50 (under 40x lens)

IP Figures

IP Result of anti-Granulin (IP:18410-1-AP, 4ug; Detection:18410-1-AP 1:800) with mouse kidney tissue lysate 4000ug.

Granulin Antibody 7 Publications

Rabbit Polyclonal| Catalog number: 18410-1-AP

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Tested Applications

Product Information

Immunogen Information

Background

Showing 1- [6] of [7] Publications

WB Figures

IHC Figures

IP Figures

Product-specific protocols

Granulin Antibody 7 Publications

Rabbit Polyclonal| Catalog number: 18410-1-AP

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Special offer: Add control/tag antibody for $99

Tested Applications

Product Information

Immunogen Information

Background