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Progranulin/PGRN Polyclonal antibody

Progranulin/PGRN Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 18410-1-AP

Synonyms

acrogranin, GEP, GP88, granulin, Granulin A Granulin 5, Granulin B Granulin 4, Granulin C Granulin 6, Granulin D Granulin 7, Granulin E, Granulin F Granulin 3, Granulin G Granulin 2, Granulins, GRN, PCDGF, PEPI, PGRN, Proepithelin



Tested Applications

Positive WB detected inA549 cells, A431 cells, HeLa cells, HEK-293 cells, PC-3 cells, human saliva, mouse heart tissue, mouse lung tissue, rat brain tissue, U-937 cells, THP-1 cells, RAW 264.7 cells
Positive IHC detected inhuman pancreas cancer tissue, human kidney tissue, human lung cancer tissue, human ovary tumor tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

18410-1-AP targets Progranulin/PGRN in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Progranulin/PGRN fusion protein Ag13178
Full Name granulin
Calculated molecular weight 64 kDa
Observed molecular weight 60-70 kDa
GenBank accession numberBC000324
Gene symbol GRN
Gene ID (NCBI) 2896
RRIDAB_10598161
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GRN, also known as PGRN or PCDGF, is a cysteine-rich protein of 68.5 kDa that is typically secreted into a highly glycosylated 88 kDa form. PGRN is a unique growth factor that plays an important role in cutaneous wound healing. It has an anti-inflammatory effect and promotes cell proliferation. When PCDGF is degraded to several 6-25 kDa fragments, called granulins (GRNs) by neutrophil proteases, a pro-inflammatory reaction occurs. PGRN is widely expressed, particularly in epithelial cells, immune cells, neurons, and chondrocytes. High levels of PGRN expression have been reported in human cancers, and its expression is closely correlated with the development and metastasis of several cancers. The recent discovery that mutations in the gene encoding for pro-granulin (GRN) cause frontotemporal lobar degeneration (FTLD), and other neurodegenerative diseases leading to dementia, has brought renewed interest in progranulin and its functions in the central nervous system.

Protocols

Product Specific Protocols
WB protocol for Progranulin/PGRN antibody 18410-1-APDownload protocol
IHC protocol for Progranulin/PGRN antibody 18410-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Transl Psychiatry

Progranulin improves neural development via the PI3K/Akt/GSK-3β pathway in the cerebellum of a VPA-induced rat model of ASD.

Authors - Lili Wang
humanIHC

J Proteome Res

Mining the gastric cancer secretome: identification of GRN as a potential diagnostic marker for early gastric cancer.

Authors - Loei Hendrick H
humanIF

J Mol Med (Berl)

Progranulin alleviates podocyte injury via regulating CAMKK/AMPK-mediated autophagy under diabetic conditions.

Authors - Di Zhou
humanWB

Front Oncol

GRN is a prognostic biomarker and correlated with immune infiltration in glioma: A study based on TCGA data

Authors - Su-Mei Xu
ratWB

Neuropharmacology

Abnormal spatiotemporal expression pattern of progranulin and neurodevelopment impairment in VPA-induced ASD rat model.

Authors - Junying Lan
ratWB,IHC,IF

Int J Mol Sci

Involvement of Progranulin and Granulin Expression in Inflammatory Responses after Cerebral Ischemia.

Authors - Ichiro Horinokita

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Sharan (Verified Customer) (06-24-2020)

The antibody detects the GRN protein but there is also a lot of cross-reactivity with non-specific antigens in HeLa lysate

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HeLa cells
Progranulin/PGRN Antibody Western Blot, validation (1:1000 dilution) in HeLa cells (Cat no:18410-1-AP)