Granulin Polyclonal antibody

• Featured Product
• KD/KO Validated

Granulin Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

9

Reviews (1)

Cat no : 18410-1-AP

Synonyms

acrogranin, GEP, GP88, granulin, Granulin A Granulin 5, Granulin B Granulin 4, Granulin C Granulin 6, Granulin D Granulin 7, Granulin E, Granulin F Granulin 3, Granulin G Granulin 2, Granulins, GRN, PCDGF, PEPI, PGRN, Proepithelin

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Validation Data Gallery

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  WB analysis using 18410-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18410-1-AP (Granulin antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of human saliva using 18410-1-AP

  human saliva were subjected to SDS PAGE followed by western blot with 18410-1-AP (Granulin antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 18410-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 18410-1-AP (Granulin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 18410-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 18410-1-AP (Granulin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U-937 using 18410-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 18410-1-AP (Granulin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of THP-1 using 18410-1-AP

  THP-1 cells were subjected to SDS PAGE followed by western blot with 18410-1-AP (Granulin antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 18410-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 18410-1-AP (Granulin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 18410-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18410-1-AP (Granulin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 18410-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 18410-1-AP (Granulin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 18410-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 18410-1-AP (Granulin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 18410-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 18410-1-AP (Granulin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse kidney using 18410-1-AP

  IP Result of anti-Granulin (IP:18410-1-AP, 4ug; Detection:18410-1-AP 1:800) with mouse kidney tissue lysate 4000ug.

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Tested Applications

• Positive WB detected in: mouse kidney tissue, human saliva, A431 cells, HeLa cells, rat brain tissue, HEK-293 cells, PC-3 cells, mouse heart tissue, mouse lung tissue, U-937 cells, THP-1 cells, rat heart tissue, rat kidney tissue
• Positive IP detected in: mouse kidney tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 5 publications below
• IF: See 2 publications below

Product Information

18410-1-AP targets Granulin in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Granulin fusion protein Ag13178
• Full Name: granulin
• Calculated molecular weight: 64 kDa
• Observed molecular weight: 68 kDa, 70-88 kDa
• GenBank accession number: BC000324
• Gene symbol: GRN
• Gene ID (NCBI): 2896
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

GRN, also known as PGRN or PCDGF, is a cysteine-rich protein of 68.5 kDa that is typically secreted into a highly glycosylated 88 kDa form. PGRN is a unique growth factor that plays an important role in cutaneous wound healing. It has an anti-inflammatory effect and promotes cell proliferation. When PCDGF is degraded to several 6-25 kDa fragments, called granulins (GRNs) by neutrophil proteases, a pro-inflammatory reaction occurs. PGRN is widely expressed, particularly in epithelial cells, immune cells, neurons, and chondrocytes. High levels of PGRN expression have been reported in human cancers, and its expression is closely correlated with the development and metastasis of several cancers. The recent discovery that mutations in the gene encoding for pro-granulin (GRN) cause frontotemporal lobar degeneration (FTLD), and other neurodegenerative diseases leading to dementia, has brought renewed interest in progranulin and its functions in the central nervous system.

Publications

Species	Application	Title
		Mol Med Rep; A method for establishing human primary gastric epithelial cell culture from fresh surgical gastric tissues.; Authors - Faisal Aziz
human	IHC	Clin Exp Dermatol; Significance of PC cell-derived growth factor and cyclin D1 expression in cutaneous squamous cell carcinoma.; Authors - Huang K K
human	WB, IHC	J Cancer Res Clin Oncol; PMID: 25527419; Authors - Faisal Aziz
mouse	IHC	Immunology; Progranulin is preferentially expressed in patients with psoriasis vulgaris and protects mice from psoriasis-like skin inflammation.; Authors - Kun Huang ; KO Validated
rat	WB,IHC,IF	Int J Mol Sci; Involvement of Progranulin and Granulin Expression in Inflammatory Responses after Cerebral Ischemia.; Authors - Ichiro Horinokita
rat	WB	Neuropharmacology; Abnormal spatiotemporal expression pattern of progranulin and neurodevelopment impairment in VPA-induced ASD rat model.; Authors - Junying Lan

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Sharan (Verified Customer) (06-24-2020)

The antibody detects the GRN protein but there is also a lot of cross-reactivity with non-specific antigens in HeLa lysate

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HeLa cells

Granulin Antibody Western Blot, validation (1:1000 dilution) in HeLa cells (Cat no:18410-1-AP)