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- KD/KO Validated
Granulin Polyclonal antibody
Granulin Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
human, mouse, rat
WB, IHC, IF, ELISA
Cat no : 18410-1-AP
Validation Data Gallery
|Positive WB detected in||A549 cells, A431 cells, HeLa cells, HEK-293 cells, PC-3 cells, human saliva, mouse heart tissue, mouse lung tissue, rat brain tissue, U-937 cells, THP-1 cells, RAW 264.7 cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Sample-dependent, check data in validation data gallery|
|KD/KO||See 1 publications below|
|WB||See 6 publications below|
|IHC||See 6 publications below|
|IF||See 2 publications below|
18410-1-AP targets Granulin in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Granulin fusion protein Ag13178|
|Calculated molecular weight||64 kDa|
|Observed molecular weight||60-70 kDa|
|GenBank accession number||BC000324|
|Gene ID (NCBI)||2896|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
GRN, also known as PGRN or PCDGF, is a cysteine-rich protein of 68.5 kDa that is typically secreted into a highly glycosylated 88 kDa form. PGRN is a unique growth factor that plays an important role in cutaneous wound healing. It has an anti-inflammatory effect and promotes cell proliferation. When PCDGF is degraded to several 6-25 kDa fragments, called granulins (GRNs) by neutrophil proteases, a pro-inflammatory reaction occurs. PGRN is widely expressed, particularly in epithelial cells, immune cells, neurons, and chondrocytes. High levels of PGRN expression have been reported in human cancers, and its expression is closely correlated with the development and metastasis of several cancers. The recent discovery that mutations in the gene encoding for pro-granulin (GRN) cause frontotemporal lobar degeneration (FTLD), and other neurodegenerative diseases leading to dementia, has brought renewed interest in progranulin and its functions in the central nervous system.
|Product Specific Protocols|
|WB protocol for Granulin antibody 18410-1-AP||Download protocol|
|Click here to view our Standard Protocols|
GRN is a prognostic biomarker and correlated with immune infiltration in glioma: A study based on TCGA data
Progranulin improves neural development via the PI3K/Akt/GSK-3β pathway in the cerebellum of a VPA-induced rat model of ASD.
J Proteome Res
Mining the gastric cancer secretome: identification of GRN as a potential diagnostic marker for early gastric cancer.
J Mol Med (Berl)
Progranulin alleviates podocyte injury via regulating CAMKK/AMPK-mediated autophagy under diabetic conditions.
Abnormal spatiotemporal expression pattern of progranulin and neurodevelopment impairment in VPA-induced ASD rat model.
Int J Mol Sci
Involvement of Progranulin and Granulin Expression in Inflammatory Responses after Cerebral Ischemia.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Sharan (Verified Customer) (06-24-2020)
The antibody detects the GRN protein but there is also a lot of cross-reactivity with non-specific antigens in HeLa lysate