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  • KD/KO Validated

PCGF6 Polyclonal antibody

PCGF6 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC

Conjugate

Unconjugated

Cat no : 24103-1-AP

Synonyms

MBLR, PCGF6, polycomb group ring finger 6, RING finger protein 134, RNF134



Tested Applications

Positive WB detected inPC-13 cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:150-1:600
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 24103-1-AP is PCGF6 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PCGF6 fusion protein Ag21124
Full Name polycomb group ring finger 6
Calculated molecular weight 352 aa, 39 kDa
Observed molecular weight 39 kDa
GenBank accession numberBC010235
Gene symbol PCGF6
Gene ID (NCBI) 84108
Conjugate Unconjugated
Form Liquid
Purification Method Antigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PCGF6, also named as MBLR or RNF134, is a 350 amino acid protein, which contains one RING-type zinc finger. PCGF6 localizes in the nucleus and is widely expressed in many tissues. PCGF6 as a transcriptional repressor may modulate the levels of histone H3K4Me3 by activating KDM5D histone demethylase.

Protocols

Product Specific Protocols
WB protocol for PCGF6 antibody 24103-1-APDownload protocol
IHC protocol for PCGF6 antibody 24103-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

PLoS Genet

MGA, L3MBTL2 and E2F6 determine genomic binding of the non-canonical Polycomb repressive complex PRC1.6.

Authors - Bastian Stielow
  • KO Validated
humanWB

Elife

Loss of MGA repression mediated by an atypical polycomb complex promotes tumor progression and invasiveness.

Authors - Haritha Mathsyaraja
mouseWB

Nat Commun

Repression of germline genes by PRC1.6 and SETDB1 in the early embryo precedes DNA methylation-mediated silencing.

Authors - Kentaro Mochizuki
  • KO Validated
mouseWB

Nat Commun

E2F6 initiates stable epigenetic silencing of germline genes during embryonic development.

Authors - Thomas Dahlet
mouse

Sci Adv

The SAM domain-containing protein 1 (SAMD1) acts as a repressive chromatin regulator at unmethylated CpG islands.

Authors - Bastian Stielow

Cell Stem Cell

SUMO Safeguards Somatic and Pluripotent Cell Identities by Enforcing Distinct Chromatin States.

Authors - Jack-Christophe Cossec

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Aktan (Verified Customer) (12-24-2019)

The blot is subcellular fractionation of the cell nuclei using increasing concentrations of salt. Last two lanes are chromatin fractions. After electrophoresis and transfer, 5%BSA in PBST was used as blocker for 1h. The primary antibody is diluted in blocker 1:500 and the blot is incubated o/n at cold. The bands are detected using licor secondary antibodies and Licor imager.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: E14Tg mouse embryonic stem cells
PCGF6 Antibody Western Blot, validation (1:500 dilution) in E14Tg mouse embryonic stem cells (Cat no:24103-1-AP)