Tested Applications
| Positive WB detected in | HEK-293 cells, HEK-293T cells, PC-13 cells, mouse liver tissue |
| Positive IP detected in | HEK-293T cells |
| Positive IHC detected in | mouse testis tissue, rat testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC (Intra) detected in | HEK-293T cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4800 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:150-1:600 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
24103-1-AP targets PCGF6 in WB, IHC, FC (Intra), IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag21124 Product name: Recombinant human PCGF6 protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 1-352 aa of BC010235 Sequence: MEGVAVVTAGSVGAAKTEGAAALPPPPPPPVSPPALTPAPAAGEEGPAPLSETGAPGCSGSRPPELEPERSLGRFRGRFEDEDEELEEEEELEEEEEEEEEDMSHFSLRLEGGRQDSEDEEERLINLSELTPYILCSICKGYLIDATTITECLHTFCKSCIVRHFYYSNRCPKCNIVVHQTQPLYNIRLDRQLQDIVYKLVINLEEREKKQMHDFYKERGLEVPKPAVPQPVPSSKGRSKKVLESVFRIPPELDMSLLLEFIGANEGTGHFKPLEKKFVRVSGEATIGHVEKFLRRKMGLDPACQVDIICGDHLLEQYQTLREIRRAIGDAAMQDGLLVLHYGLVVSPLKIT Predict reactive species |
| Full Name | polycomb group ring finger 6 |
| Calculated Molecular Weight | 352 aa, 39 kDa |
| Observed Molecular Weight | 40-50 kDa |
| GenBank Accession Number | BC010235 |
| Gene Symbol | PCGF6 |
| Gene ID (NCBI) | 84108 |
| RRID | AB_2737369 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen Affinity purified |
| UNIPROT ID | Q9BYE7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PCGF6, also named as MBLR or RNF134, is a 350 amino acid protein, which contains one RING-type zinc finger. PCGF6 localizes in the nucleus and is widely expressed in many tissues. PCGF6 as a transcriptional repressor may modulate the levels of histone H3K4Me3 by activating KDM5D histone demethylase.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for PCGF6 antibody 24103-1-AP | Download protocol |
| IHC protocol for PCGF6 antibody 24103-1-AP | Download protocol |
| IP protocol for PCGF6 antibody 24103-1-AP | Download protocol |
| WB protocol for PCGF6 antibody 24103-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The PCGF6 Polyclonal antibody (Cat# 24103-1-AP) has 13 citations published in journals including Cell Stem Cell, Nature Communications, Nucleic Acids Research, Science Advances, International Journal of Biological Sciences, Molecular Cancer Research, Journal of Virology, PLoS Genetics, Genes & Cells, and eLife. The associated research fields span epigenetic regulation, chromatin biology, germline gene silencing, stem cell identity, neurodevelopment, cancer (renal cell carcinoma, neuroblastoma), and viral reactivation.
| Species | Application | Title |
|---|---|---|
Cell Stem Cell SUMO Safeguards Somatic and Pluripotent Cell Identities by Enforcing Distinct Chromatin States. | ||
Nat Commun PCGF6 controls neuroectoderm specification of human pluripotent stem cells by activating SOX2 expression.
| ||
Nat Commun E2F6 initiates stable epigenetic silencing of germline genes during embryonic development. | ||
Nat Commun Repression of germline genes by PRC1.6 and SETDB1 in the early embryo precedes DNA methylation-mediated silencing.
| ||
Nucleic Acids Res Systematic identification of factors involved in the silencing of germline genes in mouse embryonic stem cells | ||
Nucleic Acids Res The histone acetyltransferase KAT6A is recruited to unmethylated CpG islands via a DNA binding winged helix domain |
Reviews
What customers say
One reviewer rated this antibody 4 out of 5 stars for Western Blot on E14Tg mouse embryonic stem cells at a 1:500 dilution. They performed subcellular nuclear fractionation with increasing salt concentrations, including chromatin fractions. After transfer, they blocked with 5% BSA in PBST for 1 hour, incubated the primary antibody overnight at cold temperature, and detected bands using Licor secondary antibodies with a Licor imager. The reviewer provided detailed protocol information supporting the antibody's performance in their application.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Aktan (Verified Customer) (12-24-2019) | The blot is subcellular fractionation of the cell nuclei using increasing concentrations of salt. Last two lanes are chromatin fractions. After electrophoresis and transfer, 5%BSA in PBST was used as blocker for 1h. The primary antibody is diluted in blocker 1:500 and the blot is incubated o/n at cold. The bands are detected using licor secondary antibodies and Licor imager.
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