PCGF6 Polyclonal antibody

• Featured Product
• KD/KO Validated

PCGF6 Polyclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, FC, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Publications

10

Reviews (1)

Cat no : 24103-1-AP

Synonyms

MBLR, PCGF6, polycomb group ring finger 6, RING finger protein 134, RNF134

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Validation Data Gallery

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  WB analysis of HEK-293 using 24103-1-AP

  WB result of PCGF6 antibody (24103-1-AP; 1:2400; incubated at room temperature for 1.5 hours) with sh-Control and sh-PCGF6 transfected HEK-293 cells.

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  WB analysis using 24103-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24103-1-AP (PCGF6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-13 using 24103-1-AP

  PC-13 cells were subjected to SDS PAGE followed by western blot with 24103-1-AP (PCGF6 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse testis using 24103-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24103-1-AP (PCGF6 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 24103-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24103-1-AP (PCGF6 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of HEK-293T using 24103-1-AP

  1X10^6 HEK-293T cells were intracellularly stained with 0.4 ug Anti-Human PCGF6 (24103-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

• Positive WB detected in: HEK-293 cells, PC-13 cells, HEK-293T cells, mouse liver tissue
• Positive IHC detected in: mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC detected in: HEK-293T cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4800
• Immunohistochemistry (IHC): IHC : 1:150-1:600
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 7 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below
• ChIP: See 1 publications below

Product Information

24103-1-AP targets PCGF6 in WB, IP, IHC, FC, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PCGF6 fusion protein Ag21124
• Full Name: polycomb group ring finger 6
• Calculated Molecular Weight: 352 aa, 39 kDa
• Observed Molecular Weight: 40-50 kDa
• GenBank Accession Number: BC010235
• Gene Symbol: PCGF6
• Gene ID (NCBI): 84108
• RRID: AB_2737369
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PCGF6, also named as MBLR or RNF134, is a 350 amino acid protein, which contains one RING-type zinc finger. PCGF6 localizes in the nucleus and is widely expressed in many tissues. PCGF6 as a transcriptional repressor may modulate the levels of histone H3K4Me3 by activating KDM5D histone demethylase.

Publications

Species	Application	Title
		Cell Stem Cell; SUMO Safeguards Somatic and Pluripotent Cell Identities by Enforcing Distinct Chromatin States.; Authors - Jack-Christophe Cossec
mouse		Sci Adv; The SAM domain-containing protein 1 (SAMD1) acts as a repressive chromatin regulator at unmethylated CpG islands.; Authors - Bastian Stielow
mouse	WB	Nat Commun; E2F6 initiates stable epigenetic silencing of germline genes during embryonic development.; Authors - Thomas Dahlet
mouse	WB	Nat Commun; Repression of germline genes by PRC1.6 and SETDB1 in the early embryo precedes DNA methylation-mediated silencing.; Authors - Kentaro Mochizuki ; KO Validated
human	WB,CoIP	Nat Commun; PCGF6 controls neuroectoderm specification of human pluripotent stem cells by activating SOX2 expression.; Authors - Xianchun Lan ; KD Validated
human	WB	Elife; Loss of MGA repression mediated by an atypical polycomb complex promotes tumor progression and invasiveness.; Authors - Haritha Mathsyaraja

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Aktan (Verified Customer) (12-24-2019)

The blot is subcellular fractionation of the cell nuclei using increasing concentrations of salt. Last two lanes are chromatin fractions. After electrophoresis and transfer, 5%BSA in PBST was used as blocker for 1h. The primary antibody is diluted in blocker 1:500 and the blot is incubated o/n at cold. The bands are detected using licor secondary antibodies and Licor imager.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: E14Tg mouse embryonic stem cells

PCGF6 Antibody Western Blot, validation (1:500 dilution) in E14Tg mouse embryonic stem cells (Cat no:24103-1-AP)