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- KD/KO Validated
PCK1 Polyclonal antibody
PCK1 Polyclonal Antibody for IF, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
human, mouse, rat and More (3)
WB, IP, IHC, IF, ELISA
Cat no : 16754-1-AP
|Positive WB detected in||mouse liver tissue, HepG2 cells, rat liver tissue|
|Positive IP detected in||HepG2 cells|
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunofluorescence (IF)||IF : 1:200-1:800|
|Sample-dependent, check data in validation data gallery|
|KD/KO||See 5 publications below|
|WB||See 46 publications below|
|IHC||See 5 publications below|
|IF||See 7 publications below|
|IP||See 2 publications below|
16754-1-AP targets PCK1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, bovine, goat, pig|
|Host / Isotype||Rabbit / IgG|
|Immunogen||PCK1 fusion protein Ag10261|
|Full Name||phosphoenolpyruvate carboxykinase 1 (soluble)|
|Calculated molecular weight||622 aa, 69 kDa|
|Observed molecular weight||66-69 kDa|
|GenBank accession number||BC023978|
|Gene ID (NCBI)||5105|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
PCK1(Phosphoenolpyruvate carboxykinase, cytosolic) is also named as PEPCK1 and belongs to the phosphoenolpyruvate carboxykinase [GTP] family. It catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. It is also a main control point for the regulation of gluconeogenesis. In eukaryotes there are two isozymes: a cytoplasmic one and a mitochondrial one. Defects in PCK1 are the cause of cytosolic phosphoenolpyruvate carboxykinase deficiency (C-PEPCKD).
|Product Specific Protocols|
|WB protocol for PCK1 antibody 16754-1-AP||Download protocol|
|IF protocol for PCK1 antibody 16754-1-AP||Download protocol|
|IP protocol for PCK1 antibody 16754-1-AP||Download protocol|
|Click here to view our Standard Protocols|
The gluconeogenic enzyme PCK1 phosphorylates INSIG1/2 for lipogenesis.
Glycogen metabolism regulates macrophage-mediated acute inflammatory responses.
Liver-targeted Nano-MitoPBN normalizes glucose metabolism by improving mitochondrial redox balance.
Proc Natl Acad Sci U S A
Circadian clock-dependent and -independent rhythmic proteomes implement distinct diurnal functions in mouse liver.
Prostaglandin F2α Facilitates Hepatic Glucose Production Through CaMKIIγ/p38/FoxO1-Signaling Pathway in Fasting and Obesity.
Cancer Cell Int
PCK1 activates oncogenic autophagy via down-regulation Serine phosphorylation of UBAP2L and antagonizes colorectal cancer growth
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
James (Verified Customer) (11-18-2022)
Detection of PCK1 in liver protein extracts