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PCK1 Polyclonal antibody
PCK1 Polyclonal Antibody for IF, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (3)
Applications
WB, IP, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 16754-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | mouse liver tissue, HepG2 cells, rat liver tissue |
Positive IP detected in | HepG2 cells |
Positive IF detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB |
Immunofluorescence (IF) | IF : 1:200-1:800 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 5 publications below |
WB | See 46 publications below |
IHC | See 5 publications below |
IF | See 7 publications below |
IP | See 2 publications below |
Product Information
16754-1-AP targets PCK1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, bovine, goat, pig |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | PCK1 fusion protein Ag10261 |
Full Name | phosphoenolpyruvate carboxykinase 1 (soluble) |
Calculated molecular weight | 622 aa, 69 kDa |
Observed molecular weight | 66-69 kDa |
GenBank accession number | BC023978 |
Gene symbol | PCK1 |
Gene ID (NCBI) | 5105 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PCK1(Phosphoenolpyruvate carboxykinase, cytosolic) is also named as PEPCK1 and belongs to the phosphoenolpyruvate carboxykinase [GTP] family. It catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. It is also a main control point for the regulation of gluconeogenesis. In eukaryotes there are two isozymes: a cytoplasmic one and a mitochondrial one. Defects in PCK1 are the cause of cytosolic phosphoenolpyruvate carboxykinase deficiency (C-PEPCKD).
Protocols
Product Specific Protocols | |
---|---|
WB protocol for PCK1 antibody 16754-1-AP | Download protocol |
IF protocol for PCK1 antibody 16754-1-AP | Download protocol |
IP protocol for PCK1 antibody 16754-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Nature The gluconeogenic enzyme PCK1 phosphorylates INSIG1/2 for lipogenesis.
| ||
Nat Commun Glycogen metabolism regulates macrophage-mediated acute inflammatory responses. | ||
Biomaterials Liver-targeted Nano-MitoPBN normalizes glucose metabolism by improving mitochondrial redox balance. | ||
Proc Natl Acad Sci U S A Circadian clock-dependent and -independent rhythmic proteomes implement distinct diurnal functions in mouse liver.
| ||
Diabetes Prostaglandin F2α Facilitates Hepatic Glucose Production Through CaMKIIγ/p38/FoxO1-Signaling Pathway in Fasting and Obesity. | ||
Cancer Cell Int PCK1 activates oncogenic autophagy via down-regulation Serine phosphorylation of UBAP2L and antagonizes colorectal cancer growth |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH James (Verified Customer) (11-18-2022) | Detection of PCK1 in liver protein extracts
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