• Featured Product
  • KD/KO Validated

PCK1 Polyclonal antibody

PCK1 Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 16754-1-AP

Synonyms

PCK1, PEPCK C, PEPCK1, PEPCKC, Phosphoenolpyruvate carboxykinase 1



Tested Applications

Positive WB detected inmouse liver tissue, HepG2 cells, rat liver tissue
Positive IP detected inHepG2 cells
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16754-1-AP targets PCK1 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, goat, pig, bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PCK1 fusion protein Ag10261
Full Name phosphoenolpyruvate carboxykinase 1 (soluble)
Calculated Molecular Weight 622 aa, 69 kDa
Observed Molecular Weight 66-69 kDa
GenBank Accession NumberBC023978
Gene Symbol PCK1
Gene ID (NCBI) 5105
RRIDAB_2160031
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PCK1(Phosphoenolpyruvate carboxykinase, cytosolic) is also named as PEPCK1 and belongs to the phosphoenolpyruvate carboxykinase [GTP] family. It catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. It is also a main control point for the regulation of gluconeogenesis. In eukaryotes there are two isozymes: a cytoplasmic one and a mitochondrial one. Defects in PCK1 are the cause of cytosolic phosphoenolpyruvate carboxykinase deficiency (C-PEPCKD).

Protocols

Product Specific Protocols
WB protocol for PCK1 antibody 16754-1-APDownload protocol
IF protocol for PCK1 antibody 16754-1-APDownload protocol
IP protocol for PCK1 antibody 16754-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IP

Nature

The gluconeogenic enzyme PCK1 phosphorylates INSIG1/2 for lipogenesis.

Authors - Daqian Xu
  • KD Validated
mouseWB

Nat Commun

Glycogen metabolism regulates macrophage-mediated acute inflammatory responses.

Authors - Jingwei Ma
mouseIF

Adv Healthc Mater

Mesenchymal Stem Cell Exosomes Encapsulated Oral Microcapsules for Acute Colitis Treatment.

Authors - Jingjing Gan
humanWB

Biomaterials

Liver-targeted Nano-MitoPBN normalizes glucose metabolism by improving mitochondrial redox balance.

Authors - Meiling Wu
mouseWB

Proc Natl Acad Sci U S A

Circadian clock-dependent and -independent rhythmic proteomes implement distinct diurnal functions in mouse liver.

Authors - Daniel Mauvoisin
  • KO Validated
mouseWB

Diabetes

Prostaglandin F2α Facilitates Hepatic Glucose Production Through CaMKIIγ/p38/FoxO1-Signaling Pathway in Fasting and Obesity.

Authors - Yuanyang Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

James (Verified Customer) (11-18-2022)

Detection of PCK1 in liver protein extracts

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: Liver
PCK1 Antibody Western Blot validation (1/1000 dilution) in Liver (Cat no:16754-1-AP)