|Positive WB detected in||mouse liver tissue, HepG2 cells, rat liver tissue|
|Positive IP detected in||HepG2 cells|
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
16754-1-AP targets PCK1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, goat, pig|
|Host / Isotype||Rabbit / IgG|
|Immunogen||PCK1 fusion protein Ag10261|
|Full Name||phosphoenolpyruvate carboxykinase 1 (soluble)|
|Calculated molecular weight||622 aa, 69 kDa|
|Observed molecular weight||66-69 kDa|
|GenBank accession number||BC023978|
|Gene ID (NCBI)||5105|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
PCK1(Phosphoenolpyruvate carboxykinase, cytosolic) is also named as PEPCK1 and belongs to the phosphoenolpyruvate carboxykinase [GTP] family. It catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. It is also a main control point for the regulation of gluconeogenesis. In eukaryotes there are two isozymes: a cytoplasmic one and a mitochondrial one. Defects in PCK1 are the cause of cytosolic phosphoenolpyruvate carboxykinase deficiency (C-PEPCKD).
Liver-derived fibroblast growth factor 21 mediates effects of glucagon-like peptide-1 in attenuating hepatic glucose output.
Diabetes Metab Syndr Obes
Resveratrol affects hepatic gluconeogenesis via histone deacetylase 4.
Adropin reduces blood glucose levels in mice by limiting hepatic glucose production.
J Am Soc Nephrol
Von Hippel-Lindau Acts as a Metabolic Switch Controlling Nephron Progenitor Differentiation.
J Anim Sci Biotechnol
Acta Pharmacol Sin
SL010110, a lead compound, inhibits gluconeogenesis via SIRT2-p300-mediated PEPCK1 degradation and improves glucose homeostasis in diabetic mice.