Tested Applications
| Positive WB detected in | mouse liver tissue, pig liver tissue, rat liver tissue |
| Positive IP detected in | HepG2 cells |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16754-1-AP targets PCK1/2 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| Tested Reactivity | human, mouse, rat, pig |
| Cited Reactivity | human, mouse, rat, pig, zebrafish, bovine, goat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag10261 Product name: Recombinant human PCK1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 274-622 aa of BC023978 Sequence: EGEKKYLAAAFPSACGKTNLAMMNPSLPGWKVECVGDDIAWMKFDAQGHLRAINPENGFFGVAPGTSVKTNPNAIKTIQKNTIFTNVAETSDGGVYWEGIDEPLASGVTITSWKNKEWSSEDGEPCAHPNSRFCTPASQCPIIDAAWESPEGVPIEGIIFGGRRPAGVPLVYEALSWQHGVFVGAAMRSEATAAAEHKGKIIMHDPFAMRPFFGYNFGKYLAHWLSMAQHPAAKLPKIFHVNWFRKDKEGKFLWPGFGENSRVLEWMFNRIDGKASTKLTPIGYIPKEDALNLKGLGHINMMELFSISKEFWEKEVEDIEKYLEDQVNADLPCEIEREILALKQRISQM Predict reactive species |
| Full Name | phosphoenolpyruvate carboxykinase 1 (soluble) |
| Calculated Molecular Weight | 622 aa, 69 kDa |
| Observed Molecular Weight | 66-69 kDa |
| GenBank Accession Number | BC023978 |
| Gene Symbol | PCK1 |
| Gene ID (NCBI) | 5105 |
| RRID | AB_2160031 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P35558 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PCK1(Phosphoenolpyruvate carboxykinase, cytosolic) is also named as PEPCK1 and belongs to the phosphoenolpyruvate carboxykinase [GTP] family. It catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. It is also a main control point for the regulation of gluconeogenesis. In eukaryotes there are two isozymes: a cytoplasmic one and a mitochondrial one. Defects in PCK1 are the cause of cytosolic phosphoenolpyruvate carboxykinase deficiency (C-PEPCKD). This antibody has weak cross-reactivity with PCK2 protein due to high sequence homology between PCK1 and PCK2.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for PCK1/2 antibody 16754-1-AP | Download protocol |
| IP protocol for PCK1/2 antibody 16754-1-AP | Download protocol |
| WB protocol for PCK1/2 antibody 16754-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The PCK1/2 Polyclonal antibody (Cat# 16754-1-AP) has 115 citations. It appears in high-impact journals including Nature, Cell Metabolism, Nature Communications, Nature Metabolism, Molecular Cell, Journal of Experimental Medicine, Science Advances, and PNAS, among others. Research fields span hepatic gluconeogenesis, glucose and lipid metabolism, insulin resistance, type 2 diabetes, NAFLD/NASH, and metabolic reprogramming, plus oncology (breast, prostate, colorectal, renal, and cholangiocarcinoma), ferroptosis, and immune/inflammatory regulation. Applications are predominantly Western blot, with IHC, IF, and IP.
| Species | Application | Title |
|---|---|---|
Nature The gluconeogenic enzyme PCK1 phosphorylates INSIG1/2 for lipogenesis.
| ||
Cell Metab A 5:2 intermittent fasting regimen ameliorates NASH and fibrosis and blunts HCC development via hepatic PPARα and PCK1 | ||
Cancer Commun (Lond) Simvastatin overcomes the pPCK1-pLDHA-SPRINGlac axis-mediated ferroptosis and chemo-immunotherapy resistance in AKT-hyperactivated intrahepatic cholangiocarcinoma
| ||
Nat Metab Redox-dependent liver gluconeogenesis impacts different intensity exercise in mice | ||
Nat Commun Transcriptional coactivation by EHMT2 restricts glucocorticoid-induced insulin resistance in a study with male mice |
Reviews
What customers say
The single review for this product is a 5-star rating. The user successfully detected PCK1 in liver protein extracts using Western Blot at a 1:1000 dilution, reporting clear and reliable results. Overall, the feedback indicates strong performance for WB applications with this antibody.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
James (Verified Customer) (11-18-2022) | Detection of PCK1 in liver protein extracts
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