Validation Data Gallery
|Positive WB detected in||HEK-293 cells, HeLa cells, HepG2 cells, Jurkat cells, MCF-7 cells, mouse testis tissue, NIH/3T3 cells, Raji cells, rat liver tissue|
|Positive IP detected in||HEK-293 cells|
|Positive IHC detected in||human breast cancer tissue, human gliomas tissue, human stomach cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||human breast cancer tissue, HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
The immunogen of 24036-1-AP is PCNA Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||PCNA fusion protein Ag7416|
|Full Name||proliferating cell nuclear antigen|
|Calculated molecular weight||29 kDa/31 kDa|
|Observed molecular weight||36-38 kDa|
|GenBank accession number||BC000491|
|Gene ID (NCBI)||5111|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
The proliferating cell nuclear antigen (PCNA), a protein synthesized in early G1 and S phases of the cell cycle, functions in cell cycle progression, DNA replication and DNA repair. In early S phase, PCNA exhibits granular distribution and is absent from the nucleoli; however, in late S phase, it relocates to the nucleoli. PCNA exists in two basic forms: one involved in ongoing DNA replication, which localizes specifically to the nucleus, and a second, soluble form, not implicated in constant synthesis. Interestingly, the latter form degrades in the presence of organic solvents, rendering it undetectable by histological methods in tissues using organic fixatives, and thus also providing a method of visualizing only the synthesizing form.This antibody specifically react with the 36kd human PCNA protein.
|Product Specific Protocols|
|WB protocol for PCNA antibody 24036-1-AP||Download protocol|
|IHC protocol for PCNA antibody 24036-1-AP||Download protocol|
|IF protocol for PCNA antibody 24036-1-AP||Download protocol|
|IP protocol for PCNA antibody 24036-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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