Tested Applications
| Positive WB detected in | HepG2 cells, rat brain tissue, L02 cells, 4T1 cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
55206-1-AP targets PCSK9 in WB, IHC, IF, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, hamster |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | proprotein convertase subtilisin/kexin type 9 |
| Calculated Molecular Weight | 74 kDa |
| Observed Molecular Weight | 58-62 kDa, 72-78 kDa |
| GenBank Accession Number | NM_174936 |
| Gene Symbol | PCSK9 |
| Gene ID (NCBI) | 255738 |
| RRID | AB_10918272 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8NBP7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Proprotein convertase subtilisin/kexin type 9 (PCSK9) is a crucial protein governing the circulating levels of low density lipoprotein-cholesterol (LDL-C), by virtue of its pivotal role in the degradation of the LDL receptor (LDLR). PCSK9 is expressed in the kidney and lung. It is synthesized as a 72 kDa immature precursor that undergoes autocatalytic cleavage in the endoplasmic reticulum to generate a 63 kDa mature protein. The cleaved N-terminal fragment remains associated with the mature protein and is necessary for its secretion, allowing it to circulate in the blood. The ability of PCSK9 to regulate a diverse group of cell-surface proteins hinted that it might also be able to influence additional membrane proteins that are important in anti-tumour immune responses. Targeting PCSK9 to treat cancer is also attractive because two neutralizing antibodies against it, evolocumab and alirocumab, have already been approved for human clinical use to lower cholesterol levels. (PMID: 30522786, PMID: 22493497)
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for PCSK9 antibody 55206-1-AP | Download protocol |
| WB protocol for PCSK9 antibody 55206-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The PCSK9 Polyclonal antibody (Cat# 55206-1-AP) has 73 citations across journals including Nature, Nature Communications, Metabolism, Acta Pharmacologica Sinica, Journal of Nanobiotechnology, Cell Reports, and International Journal of Molecular Sciences. Research fields span lipid metabolism, atherosclerosis, cardiovascular disease, cancer (hepatocellular, lung, breast, pancreatic), neurodegeneration, reproductive health, renal disease, and novel PCSK9-targeted drug development.
| Species | Application | Title |
|---|---|---|
Cancer Commun (Lond) Lipid metabolism reprograming by SREBP1-PCSK9 targeting sensitizes pancreatic cancer to immunochemotherapy
| ||
Exp Hematol Oncol PCSK9 promotes tumor growth by inhibiting tumor cell apoptosis in hepatocellular carcinoma.
| ||
Metabolism Role of PCSK9 in lipid metabolic disorders and ovarian dysfunction in polycystic ovary syndrome. | ||
Metabolism CADD-engineered peptide protacs efficiently target PCSK9 for hypercholesterolemia in vivo. |
Reviews
What customers say
The single review for this antibody reports excellent performance in immunohistochemistry on hepatic tissue. The user rated it 5 out of 5 stars and noted that it works very well at a 1/100 dilution, particularly at pH 6. Overall, the feedback highlights strong staining results and reliable performance for IHC applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Malak (Verified Customer) (11-30-2020) | The antibody works very well in IHC staining especially at pH6 and 1/100 dilution
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