CCM3/PDCD10 Polyclonal antibody

• Featured Product
• KD/KO Validated

CCM3/PDCD10 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

12

Cat no : 10294-2-AP

Synonyms

CCM3, PDCD10, programmed cell death 10, TFAR15

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Validation Data Gallery

• 

  WB analysis of K-562 using 10294-2-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 10294-2-AP (CCM3/PDCD10 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat skin using 10294-2-AP

  rat skin tissue were subjected to SDS PAGE followed by western blot with 10294-2-AP (CCM3/PDCD10 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse skeletal muscle using 10294-2-AP

  IP Result of anti-CCM3/PDCD10 (IP:10294-2-AP, 3ug; Detection:10294-2-AP 1:300) with mouse skeletal muscle tissue lysate 7500ug.

• 

  IHC staining of human colon cancer using 10294-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 10294-2-AP (CCM3/PDCD10 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 10294-2-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 10294-2-AP (CCM3/PDCD10 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 10294-2-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 10294-2-AP (CCM3/PDCD10 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon cancer using 10294-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 10294-2-AP (CCM3/PDCD10 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon cancer using 10294-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 10294-2-AP (CCM3/PDCD10 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of mouse brain using 10294-2-AP

  WB and IF result of CCM3 antibody (courtesy of Dr. Shah, University of Muenster). IF note: E15 mouse brain cortex was PFA fixed, the sections were antigen retrieved with Na-citrate buffer (pH 6) and blocked with NGS and Triton X100. 1:150 dilution of CCM3 antibody was used with overnight incubation of sections at 4 degrees. Secondary anti-Rabbit conjugated to AF488 was used to detect the staining.

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Tested Applications

• Positive WB detected in: K-562 cells, rat skin tissue
• Positive IP detected in: mouse skeletal muscle tissue
• Positive IHC detected in: human colon cancer tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: mouse brain

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:100-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 8 publications below
• WB: See 11 publications below
• IHC: See 3 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Product Information

10294-2-AP targets CCM3/PDCD10 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CCM3/PDCD10 fusion protein Ag0348
• Full Name: programmed cell death 10
• Calculated molecular weight: 25 kDa
• Observed molecular weight: 25-30 kDa
• GenBank accession number: BC002506
• Gene symbol: PDCD10
• Gene ID (NCBI): 11235
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

PDCD10, also named CCM3 and TFAR15, belongs to the PDCD10 family. PDCD10 promotes cell proliferation, increases MAPK activity and modulates apoptotic pathways. PDCD10 is important for cell migration and for normal structure and assembly of the Golgi complex. PDCD10 is required for normal angiogenesis, vasculogenesis and hematopoiesis during embryonic development, moreover, defects in PDCD10 showed abnormal cardiovascular development. Catalog#10294-2-AP is a rabbit polyclonal antibody raised against the full-length PDCD10 of human origin.

Publications

Species	Application	Title
human	WB,IHC,IF,IP	Cell Death Dis; Programmed cell death 10 promotes metastasis and epithelial-mesenchymal transition of hepatocellular carcinoma via PP2Ac-mediated YAP activation.; Authors - Bo Sun ; KD Validated
mouse,rat	WB,IF	Brain Res Bull; Cerebral cavernous malformation 3 relieves subarachnoid hemorrhage-induced neuroinflammation in rats through inhibiting NF-kB signaling pathway.; Authors - Wei Peng
		Toxicol Lett; Arsenic-induced anti-angiogenesis via miR-425-5p-regulated CCM3.; Authors - Yanfang Gao
human	WB,IHC	J Biol Chem; PDCD10/CCM3 acts downstream of {gamma}-protocadherins to regulate neuronal survival.; Authors - Lin Chengyi C ; KD Validated
human	WB	J Biol Chem; Adaptor protein cerebral cavernous malformation 3 (CCM3) mediates phosphorylation of the cytoskeletal proteins ezrin/radixin/moesin by mammalian Ste20-4 to protect cells from oxidative stress.; Authors - Fidalgo Miguel M ; KD Validated
rat	WB	Biochem J; A novel non-canonical mechanism of regulation of MST3 (mammalian Sterile20-related kinase 3).; Authors - Fuller Stephen J SJ

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