|Positive WB detected in||MCF-7 cells, PC-3 cells, Raji cells|
|Positive IP detected in||MCF-7 cells|
|Positive IHC detected in||human colon cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
10294-2-AP targets CCM3/PDCD10 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||CCM3/PDCD10 fusion protein Ag0348|
|Full Name||programmed cell death 10|
|Calculated molecular weight||25 kDa|
|Observed molecular weight||25-30 kDa|
|GenBank accession number||BC002506|
|Gene ID (NCBI)||11235|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
PDCD10, also named CCM3 and TFAR15, belongs to the PDCD10 family. PDCD10 promotes cell proliferation, increases MAPK activity and modulates apoptotic pathways. PDCD10 is important for cell migration and for normal structure and assembly of the Golgi complex. PDCD10 is required for normal angiogenesis, vasculogenesis and hematopoiesis during embryonic development, moreover, defects in PDCD10 showed abnormal cardiovascular development. Catalog#10294-2-AP is a rabbit polyclonal antibody raised against the full-length PDCD10 of human origin.
Nat Cell Biol
CCM3 is a gatekeeper in focal adhesions regulating mechanotransduction and YAP/TAZ signalling.
J Clin Invest
Mutations in 2 distinct genetic pathways result in cerebral cavernous malformations in mice.
Ccm3, a gene associated with cerebral cavernous malformations, is required for neuronal migration.
Aging (Albany NY)
PDCD10 promotes the aggressive behaviors of pituitary adenomas by up-regulating CXCR2 and activating downstream AKT/ERK signaling
J Cell Sci
CCM3/PDCD10 stabilizes GCKIII proteins to promote Golgi assembly and cell orientation.
A single-center study on 140 patients with cerebral cavernous malformations: 28 new pathogenic variants and functional characterization of a PDCD10 large deletion .