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  • KD/KO Validated

CCM3/PDCD10 Polyclonal antibody

CCM3/PDCD10 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 10294-2-AP

Synonyms

CCM3, CCM3/PDCD10, PDCD10, programmed cell death 10, TFAR15



Tested Applications

Positive WB detected inMCF-7 cells, PC-3 cells, Raji cells
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10294-2-AP targets CCM3/PDCD10 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CCM3/PDCD10 fusion protein Ag0348
Full Name programmed cell death 10
Calculated molecular weight 25 kDa
Observed molecular weight 25-30 kDa
GenBank accession numberBC002506
Gene symbol PDCD10
Gene ID (NCBI) 11235
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PDCD10, also named CCM3 and TFAR15, belongs to the PDCD10 family. PDCD10 promotes cell proliferation, increases MAPK activity and modulates apoptotic pathways. PDCD10 is important for cell migration and for normal structure and assembly of the Golgi complex. PDCD10 is required for normal angiogenesis, vasculogenesis and hematopoiesis during embryonic development, moreover, defects in PDCD10 showed abnormal cardiovascular development. Catalog#10294-2-AP is a rabbit polyclonal antibody raised against the full-length PDCD10 of human origin.

Protocols

Product Specific Protocols
WB protocol for CCM3/PDCD10 antibody 10294-2-APDownload protocol
IHC protocol for CCM3/PDCD10 antibody 10294-2-APDownload protocol
IP protocol for CCM3/PDCD10 antibody 10294-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nat Cell Biol

CCM3 is a gatekeeper in focal adhesions regulating mechanotransduction and YAP/TAZ signalling.

Authors - Shan Wang
  • KO Validated
human,mouseWB,IHC

J Clin Invest

Mutations in 2 distinct genetic pathways result in cerebral cavernous malformations in mice.

Authors - Chan Aubrey C AC
  • KD Validated
mouseWB

Development

Ccm3, a gene associated with cerebral cavernous malformations, is required for neuronal migration.

Authors - Angeliki Louvi
  • KO Validated
human,ratWB,IF

J Cell Sci

CCM3/PDCD10 stabilizes GCKIII proteins to promote Golgi assembly and cell orientation.

Authors - Fidalgo Miguel M
  • KD Validated
humanWB

Hum Mutat

A single-center study on 140 patients with cerebral cavernous malformations: 28 new pathogenic variants and functional characterization of a PDCD10 large deletion ​.

Authors - Grazia Nardella
mouseWB

FASEB J

Connexin 43 gap junctions contribute to brain endothelial barrier hyperpermeability in familial cerebral cavernous malformations type III by modulating tight junction structure.

Authors - Allison M Johnson
  • KD Validated