PDGFR beta Polyclonal antibody

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  WB analysis using 32335-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 32335-1-AP (PDGFR beta antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

PDGFR beta Polyclonal Antibody for WB, ELISA

Cat No. 32335-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, ELISA

Synonyms

Pdgfr, EC:2.7.10.1, CD140b, CD140 antigen-like family member B, Beta-type platelet-derived growth factor receptor

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Tested Applications

• Positive WB detected in: C2C12 cells, NIH/3T3 cells, C6 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

32335-1-AP targets PDGFR beta in WB, ELISA applications and shows reactivity with mouse, rat samples.

• Tested Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: platelet derived growth factor receptor, beta polypeptide
• Calculated Molecular Weight: 123kDa
• Observed Molecular Weight: 180 kDa
• GenBank Accession Number: NM_008809.2
• Gene Symbol: PDGFR beta
• Gene ID (NCBI): 18596
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: P05622-1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The Platelet-Derived Growth Factor Receptor Beta (PDGFRβ) is a receptor tyrosine kinase that plays a crucial role in various cellular processes, including cell proliferation, migration, and survival. In mice, PDGFRβ is particularly significant due to its expression in pericytes, which are cells that reside on the walls of capillaries and play a vital role in maintaining vascular stability and integrity (PMID: 20738866). In the central nervous system (CNS) of mice, PDGFRβ is predominantly expressed in pericytes and vascular smooth muscle cells. It is involved in the formation and maintenance of the blood-brain barrier, as well as in the regulation of angiogenesis and vascular stability. Research in mouse models has shown that PDGFRβ signaling is crucial for tissue repair and functional recovery after stroke. Mice with disrupted PDGFRβ signaling exhibit delayed recovery and increased infarction volume following cerebral ischemia (PMID: 21952111). The observed MW is about 180 kDa, which may be caused by post-translational modification.