PDH E1 Alpha Polyclonal antibody

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  WB analysis using 18068-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18068-1-AP (PDH E1 Alpha antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK293 cells using 18068-1-AP

  WB result of PDHA1 antibody (18068-1-AP, 1:1000) with si-control and si-PDHA1 transfected HEK293 cells.

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  WB analysis using 18068-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18068-1-AP (PDH E1α antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 18068-1-AP

  IP result of anti-PDH E1 Alpha (IP:18068-1-AP, 4ug; Detection:18068-1-AP 1:30000) with HeLa cells lysate 920 ug.

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  IHC staining of human liver cancer using 18068-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18068-1-AP (PDH E1 Alpha antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 18068-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18068-1-AP (PDH E1 Alpha antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 18068-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PDH E1 Alpha antibody (18068-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

• Featured Product
• KD/KO Validated

PDH E1 Alpha Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 18068-1-AP

Publications(86)

Reviews (3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

PDH E1α, PDHA1, EC:1.2.4.1, PDHA, PDHA 1

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Tested Applications

• Positive WB detected in: HeLa cells, HEK293 cells, mouse brain tissue, rat brain tissue, rat kidney tissue
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 70 publications below
• IHC: See 5 publications below
• IF: See 9 publications below
• IP: See 2 publications below

Product Information

18068-1-AP targets PDH E1 Alpha in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, chicken, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag12701

  Product name: Recombinant human PDHA1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 41-390 aa of BC002406

  Sequence: CDLHRLEEGPPVTTVLTREDGLKYYRMMQTVRRMELKADQLYKQKIIRGFCHLCDGQEACCVGLEAGINPTDHLITAYRAHGFTFTRGLSVREILAELTGRKGGCAKGKGGSMHMYAKNFYGGNGIVGAQVPLGAGIALACKYNGKDEVCLTLYGDGAANQGQIFEAYNMAALWKLPCIFICENNRYGMGTSVERAAASTDYYKRGDFIPGLRVDGMDILCVREATRFAAAYCRSGKGPILMELQTYRYHGHSMSDPGVSYRTREEIQEVRSKSDPIMLLKDRMVNSNLASVEELKEIDVEVRKEIEDAAQFATADPEPPLEELGYHIYSSDPPFEVRGANQWIKFKSVS

• Full Name: pyruvate dehydrogenase (lipoamide) alpha 1
• Calculated Molecular Weight: 43 kDa
• Observed Molecular Weight: 39-48 kDa
• GenBank Accession Number: BC002406
• Gene Symbol: PDHA1
• Gene ID (NCBI): 5160
• RRID: AB_2162931
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P08559
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PDHA1(Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial) is also named as PHE1A.It is one of the 3 enzymes of the pyruvate dehydrogenase complex which is a nuclear-encoded mitochondrial matrix multienzyme complex that provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle by catalyzing the irreversible conversion of pyruvate into acetyl-CoA(PMID:7853374).It has 4 isoforms produced by alternative splicing with the molecular mass of 39-48 kDa. Defects in PDHA1 are a cause of pyruvate dehydrogenase E1-alpha deficiency (PDHAD) and X-linked Leigh syndrome (X-LS).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB,IP	Cell Res; Hypoxia induces mitochondrial protein lactylation to limit oxidative phosphorylation; Authors - Yunzi Mao
human	WB	Cell Res; AMPK targets PDZD8 to trigger carbon source shift from glucose to glutamine; Authors - Mengqi Li
mouse	WB	Cell Metab; Acetate enables metabolic fitness and cognitive performance during sleep disruption; Authors - Qinqin He
mouse	WB	Nat Cell Biol; RIP3 targets pyruvate dehydrogenase complex to increase aerobic respiration in TNF-induced necroptosis; Authors - ; KO Validated
rat	WB	Cell Stem Cell; A small-molecule cocktail promotes mammalian cardiomyocyte proliferation and heart regeneration.; Authors - Jianyong Du
human	IF	Cell Stem Cell; Mechano-oncogenic cytoskeletal remodeling drives leukemic transformation with mitochondrial vesicle-mediated STING activation; Authors - Zemin Song

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

kes (Verified Customer) (01-17-2022)

I used it for WB(1:1000) and got nice bands.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human Liver cells

PDH E1 Alpha Antibody Western Blot validation (1:1000 dilution) in Human Liver cells (Cat no:18068-1-AP)

FH

David (Verified Customer) (09-03-2019)

Good detection with 30µg of protein and dilution at 1/1000

• Applications: Western Blot,
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: Brain

PDH E1 Alpha Antibody Western Blot, validation (1/1000 dilution) in Brain (Cat no:18068-1-AP)

FH

Svitlana (Verified Customer) (11-02-2017)

Loading:per well 15 ug/ulGradient gel 4-12%Transfer:Immobilon-FL transfer membranes (Millipore) O/N at 30V, 4CBlocking:SEA Block Blocking Buffer 1hImaging:Odyssey CLx Imaging

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Whole tissue homogenate (human, mouse), mitochondria (human, mouse), whole cell homogenate (rat cell line), mitochondria (rat cell line).

PDH E1 Alpha Antibody Western Blot validation (1:1000 dilution) in Whole tissue homogenate (human, mouse), mitochondria (human, mouse), whole cell homogenate (rat cell line), mitochondria (rat cell line). (Cat no:18068-1-AP)