PDIA6 Polyclonal antibody

PDIA6 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 18233-1-AP

Synonyms

ER protein 5, ERP5, P5, PDIA6, Protein disulfide isomerase A6, Protein disulfide isomerase P5, TXNDC7



Tested Applications

Positive WB detected inrat kidney tissue, mouse heart tissue, mouse liver tissue, mouse kidney tissue, mouse lung tissue, HeLa cells, L02 cells, HEK-293 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman stomach tissue, human spleen tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:1000-1:6000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 18233-1-AP is PDIA6 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PDIA6 fusion protein Ag12940
Full Name protein disulfide isomerase family A, member 6
Calculated molecular weight 48 kDa, 54 kDa
Observed molecular weight 48-54 kDa
GenBank accession numberBC001312
Gene symbol PDIA6
Gene ID (NCBI) 10130
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PDIA6(Protein disulfide-isomerase A6) is also named as ERP5, P5, TXNDC7 and belongs to the protein disulfide isomerase family.It is one of the endoplasmic reticulum (ER) resident proteins that catalyze formation, reduction, and isomerization of disulfide bonds in proteins and are thought to play a role in folding of disulfide-bonded proteins(PMID:7590364).It has 2 isoforms produced by alternative splicing.This antibody is specific to PDIA6.

Protocols

Product Specific Protocols
WB protocol for PDIA6 antibody 18233-1-APDownload protocol
IHC protocol for PDIA6 antibody 18233-1-APDownload protocol
IF protocol for PDIA6 antibody 18233-1-APDownload protocol
IP protocol for PDIA6 antibody 18233-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

PLoS One

Quantitative Proteomic Profiling the Molecular Signatures of Annexin A5 in Lung Squamous Carcinoma Cells.

Authors - Bing Sun
mouseWB

J Ethnopharmacol

Astragalus membranaceus and Salvia miltiorrhiza ameliorates cyclosporin A-induced chronic nephrotoxicity through the "gut-kidney axis".

Authors - Cong Han
mouseWB

Int J Biochem Cell Biol

The alteration of protein profile induced by cigarette smoking via oxidative stress in mice epididymis.

Authors - Zhu Zijue Z
humanWB,IF

Oncotarget

PDIA6 promotes the proliferation of HeLa cells through activating the Wnt/β-catenin signaling pathway.

Authors - Huijun Gao
humanWB,IHC

Oncotarget

Expression of protein disulfide isomerase family members correlates with tumor progression and patient survival in ovarian cancer.

Authors - Soma Samanta
mouseWB

Mol Metab

A point mutation in the Pdia6 gene results in loss of pancreatic β-cell identity causing overt diabetes.

Authors - Nirav Florian Chhabra