PDK1 Polyclonal antibody

PDK1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 18262-1-AP

Synonyms

PDH kinase 1, PDHK1, PDK1, Pyruvate dehydrogenase kinase isoform 1



Tested Applications

Positive WB detected inmouse heart tissue, HEK-293 cells, NIH/3T3 cells, mouse liver tissue, rat liver tissue
Positive IHC detected inhuman liver cancer tissue, human heart tissue, human kidney tissue, human breast cancer tissue, mouse kidney tissue, rat kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

18262-1-AP targets PDK1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PDK1 fusion protein Ag13121
Full Name pyruvate dehydrogenase kinase, isozyme 1
Calculated molecular weight 436 aa, 49 kDa
Observed molecular weight 46-55 kDa
GenBank accession numberBC039158
Gene symbol PDK1
Gene ID (NCBI) 5163
RRIDAB_10598310
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PDK1(pyruvate dehydrogenase kinase isoform 1) is also named as PDHK1 and belongs to the PDK/BCKDK protein kinase family. It inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism (PMID:17683942). Starvation increases islet PDK activity (PMID:11723055). PDK1 has 2 isoforms with the MW of 49 and 52 kD, and a 28aa-transit-peptide in the N-terminal can be removed in the mature form. This antibody is specific to PDK1.

Protocols

Product Specific Protocols
WB protocol for PDK1 antibody 18262-1-APDownload protocol
IHC protocol for PDK1 antibody 18262-1-APDownload protocol
IF protocol for PDK1 antibody 18262-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IF

Cardiovasc Res

Inhibition of PGK1 attenuates autoimmune myocarditis by reprogramming CD4+ T cells metabolism

Authors - Yang Lu
mouseWB,IF

Cell Death Dis

Pyruvate dehydrogenase kinase 1 protects against neuronal injury and memory loss in mouse models of diabetes

Authors - Yuan Yao
mouseWB

Bioact Mater

Polydopamine-mediated graphene oxide and nanohydroxyapatite-incorporated conductive scaffold with an immunomodulatory ability accelerates periodontal bone regeneration in diabetes.

Authors - Yazhen Li
humanWB,IHC

Cancer Lett

PINK1 deficiency in gastric cancer compromises mitophagy, promotes the Warburg effect, and facilitates M2 polarization of macrophages.

Authors - Ying Xu
humanWB

Redox Biol

Impairment of Akt activity by CYP2E1 mediated oxidative stress is involved in chronic ethanol-induced fatty liver.

Authors - Tao Zeng
humanWB

Cancers (Basel)

HDAC2- and EZH2-Mediated Histone Modifications Induce PDK1 Expression through miR-148a Downregulation in Breast Cancer Progression and Adriamycin Resistance.

Authors - Yunxia Xie