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PDLIM7 Polyclonal antibody

PDLIM7 Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 17080-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, ELISA

ENIGMA, LIM mineralization protein, LMP1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

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Tested Applications

Positive WB detected inK-562 cells, HeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inmouse skeletal muscle tissue, human colon, human kidney tissue, human liver tissue, human ovary tissue, human skin tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17080-1-AP targets PDLIM7 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PDLIM7 fusion protein Ag1334 Predict reactive species
Full Name PDZ and LIM domain 7 (enigma)
Calculated Molecular Weight 50 kDa
Observed Molecular Weight 48-50 kDa
GenBank Accession NumberBC014521
Gene Symbol PDLIM7
Gene ID (NCBI) 9260
RRIDAB_2161788
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NR12
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for PDLIM7 antibody 17080-1-APDownload protocol
IHC protocol for PDLIM7 antibody 17080-1-APDownload protocol
IP protocol for PDLIM7 antibody 17080-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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