PDLIM7 Polyclonal antibody

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  WB analysis of HeLa using 17080-1-AP

  WB result of PDLIM7 antibody (17080-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PDLIM7 transfected HeLa cells.

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  WB analysis using 17080-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17080-1-AP (PDLIM7 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 17080-1-AP

  IP result of anti-PDLIM7 (IP:17080-1-AP, 4ug; Detection:17080-1-AP 1:5000) with HeLa cells lysate 1200 ug.

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  IHC staining of mouse skeletal muscle using 17080-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 17080-1-AP (PDLIM7 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 17080-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 17080-1-AP (PDLIM7 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human colon using 17080-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon slide using 17080-1-AP (PDLIM7 antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skeletal muscle using 17080-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 17080-1-AP (PDLIM7 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary using 17080-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17080-1-AP (PDLIM7 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human kidney using 17080-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17080-1-AP (PDLIM7 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human spleen using 17080-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17080-1-AP (PDLIM7 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human spleen using 17080-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17080-1-AP (PDLIM7 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human ovary using 17080-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17080-1-AP (PDLIM7 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 17080-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17080-1-AP (PDLIM7 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human skin using 17080-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 17080-1-AP (PDLIM7 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human skin using 17080-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 17080-1-AP (PDLIM7 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human liver using 17080-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 17080-1-AP (PDLIM7 antibody) at dilution of 1:100 (under 10x lens).

• Featured Product
• KD/KO Validated

PDLIM7 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 17080-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, ELISA

Synonyms

ENIGMA, LIM mineralization protein, LMP1

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Tested Applications

• Positive WB detected in: K-562 cells, HeLa cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: mouse skeletal muscle tissue, human colon, human kidney tissue, human liver tissue, human ovary tissue, human skin tissue, human spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

17080-1-AP targets PDLIM7 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag1334

  Product name: Recombinant human PDLIM7 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-222 aa of BC014521

  Sequence: MDSFKVVLEGPAPWGFRLQGGKDFNVPLSISRLTPGGKAAQAGVAVGDWVLSIDGENAGSLTHIEAQNKIRACGERLSLGLSRAQPVQSKPQKASAPAADPPRYTFAPSVSLNKTARPFGAPPPADSAPQQNGQPLRPLVPDASKQRLMENTEDWRPRPGTGQSRSFRILAHLTGTEFMQDPDEEHLKKSREKYVLELQSPRYTRLRDWHHQRSAHVLNVQS

• Full Name: PDZ and LIM domain 7 (enigma)
• Calculated Molecular Weight: 50 kDa
• Observed Molecular Weight: 48-50 kDa
• GenBank Accession Number: BC014521
• Gene Symbol: PDLIM7
• Gene ID (NCBI): 9260
• RRID: AB_2161788
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9NR12
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.