PDZD8 Polyclonal antibody

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  WB analysis using 25512-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25512-1-AP (PDZD8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

PDZD8 Polyclonal Antibody for WB, ELISA

Cat No. 25512-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, ELISA

Synonyms

PDZ domain containing 8, PDZD8, PDZK8

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Tested Applications

• Positive WB detected in: HCT 116 cells, HEK-293 cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 2 publications below

Product Information

25512-1-AP targets PDZD8 in WB, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PDZD8 fusion protein Ag21914
• Full Name: PDZ domain containing 8
• Calculated Molecular Weight: 1154 aa, 129 kDa
• Observed Molecular Weight: 150-160 kDa
• GenBank Accession Number: BC028375
• Gene Symbol: PDZD8
• Gene ID (NCBI): 118987
• RRID: AB_2880110
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8NEN9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PDZD8 (PDZ domain containing 8) is an SMP (synaptotagmin-like mitochondrial-lipid-binding) domain-containing protein belonging to the tubular lipid-binding protein (TULIP) superfamily of lipid transfer proteins (PMID: 37961523). PDZD8 is an integral ER transmembrane protein present at ER-mitochondria contacts (PMID: 29097544). It is involved in regulating ER-mitochondria associations, mitochondrial calcium ion homeostasis, and lipid transport between the ER and late endosomes/lysosomes (PMID: 37247681; 29097544; 33912962).

Publications

Species	Application	Title
human	IF	bioRxiv; Development of a signal-integrating reporter to monitor mitochondria-ER contacts; Authors - Zheng Yang
human	IF	ACS Synth Biol; Development of a Signal-integrating Reporter to Monitor Mitochondria-ER Contacts; Authors - Zheng Yang
human	WB	Nat Commun; ER-mitochondria contacts mediate lipid radical transfer via RMDN3/PTPIP51 phosphorylation to reduce mitochondrial oxidative stress; Authors - Isshin Shiiba