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  • KD/KO Validated

PERK/EIF2AK3 Polyclonal antibody

PERK/EIF2AK3 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 20582-1-AP

Synonyms

EIF2AK3, HRI, HsPEK, Pancreatic eIF2 alpha kinase, PEK, PERK, PERK/EIF2AK3, WRS



Tested Applications

Positive WB detected inHEK-293 cells, HepG2 cells, MCF-7 cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

20582-1-AP targets PERK/EIF2AK3 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, bovine, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name eukaryotic translation initiation factor 2-alpha kinase 3
Calculated molecular weight 125 kDa
Observed molecular weight 140 kDa
GenBank accession numberNM_004836
Gene symbol EIF2AK3
Gene ID (NCBI) 9451
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PERK, also named as EIF2AK3 or PEK, belongs to the protein kinase superfamily, Ser/Thr protein kinase family and GCN2 subfamily. It acts potentially as a metabolic sensor in the INS-secreting beta-cells to modulate the trafficking and quality control of pro-INS in the ER relative to the physiological demands for circulating INS (PMID:20530744). PERK and EIF2AK4 also have a functional role on regulating translation under non-stressed conditions, apart from their long established roles as stress kinases (PMID: 21638082). Defects in EIF2AK3 are the cause of Wolcott-Rallison syndrome (WRS). The molecular mass of PERK is 125 kDa and can be detected as 140 kDa or 170 kDa due to some modifications such as glycosylation and phosphorylation (PMID: 20851775, 24371088, 21703327). This antibody is specific to PERK.

Protocols

Product Specific Protocols
WB protocol for PERK/EIF2AK3 antibody 20582-1-APDownload protocol
IHC protocol for PERK/EIF2AK3 antibody 20582-1-APDownload protocol
IF protocol for PERK/EIF2AK3 antibody 20582-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nat Commun

Thbs1 induces lethal cardiac atrophy through PERK-ATF4 regulated autophagy.

Authors - Davy Vanhoutte
humanWB

Cell Death Discov

Cysteine dioxygenase 1 attenuates the proliferation via inducing oxidative stress and integrated stress response in gastric cancer cells

Authors - Gang Ma
  • KD Validated
humanWB

J Med Chem

Cytotoxic BODIPY-Appended Half-Sandwich Iridium(III) Complex Forms Protein Adducts and Induces ER Stress.

Authors - Robin Ramos
humanWB

Biochem Pharmacol

The organic arsenical-derived thioredoxin and glutathione system inhibitor ACZ2 induces apoptosis and autophagy in gastric cancer via ROS-dependent ER stress

Authors - Yi Li
mouseWB

Chemosphere

MCLR induces dysregulation of calcium homeostasis and endoplasmic reticulum stress resulting in apoptosis in Sertoli cells.

Authors - Sujuan Zhao
mouseWB

Stem Cell Res Ther

Melatonin suppresses ER stress-dependent proapoptotic effects via AMPK in bone mesenchymal stem cells during mitochondrial oxidative damage.

Authors - Chongxi Fan

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Maria (Verified Customer) (02-10-2021)

PERK in human primary fibroblasts. 12% TGX gel. Blocking 1h RT 3% BSA in PBST (0.1% Tween). Primary incubation 16h 4ºC 1:1000. Secondary 1:5000 Goat anti-rabbit HRP 1h RT.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Human Primary Fibroblasts
PERK/EIF2AK3 Antibody Western Blot validation (1:1000 dilution) in Human Primary Fibroblasts (Cat no:20582-1-AP)
FH

Aeid (Verified Customer) (11-12-2018)

Works fine for HEK293 but not for MEF or INS1 cells

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HEK293, MEF, INS1