PERK/EIF2AK3 Polyclonal antibody

• Featured Product
• KD/KO Validated

PERK/EIF2AK3 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

42

Reviews (2)

Cat no : 20582-1-AP

Synonyms

EIF2AK3, HRI, HsPEK, Pancreatic eIF2 alpha kinase, PEK, PERK, PERK/EIF2AK3, WRS

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Validation Data Gallery

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  WB analysis using 20582-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20582-1-AP (PERK/EIF2AK3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas using 20582-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 20582-1-AP (PERK/EIF2AK3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas using 20582-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 20582-1-AP (PERK/EIF2AK3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 20582-1-AP

  Immunofluorescent analysis of HeLa cells using 20582-1-AP (PERK/EIF2AK3 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, mouse pancreas tissue, HepG2 cells, MCF-7 cells
• Positive IHC detected in: human pancreas tissue, human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:10-1:100

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 41 publications below
• IHC: See 1 publications below

Product Information

20582-1-AP targets PERK/EIF2AK3 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: bovine, human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: eukaryotic translation initiation factor 2-alpha kinase 3
• Calculated molecular weight: 125 kDa
• Observed molecular weight: 140 kDa
• GenBank accession number: NM_004836
• Gene symbol: EIF2AK3
• Gene ID (NCBI): 9451
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

PERK, also named as EIF2AK3 or PEK, belongs to the protein kinase superfamily, Ser/Thr protein kinase family and GCN2 subfamily. It acts potentially as a metabolic sensor in the INS-secreting beta-cells to modulate the trafficking and quality control of pro-INS in the ER relative to the physiological demands for circulating INS (PMID:20530744). PERK and EIF2AK4 also have a functional role on regulating translation under non-stressed conditions, apart from their long established roles as stress kinases (PMID: 21638082). Defects in EIF2AK3 are the cause of Wolcott-Rallison syndrome (WRS). The molecular mass of PERK is 125 kDa and can be detected as 140 kDa or 170kDa due to some modifications such as glycosylation and phosphorylation (PMID: 20851775, 24371088, 21703327). This antibody is specific to PERK.

Publications

Species	Application	Title
human,mouse	WB	Br J Pharmacol; Betulinic acid alleviates endoplasmic reticulum stress-mediated NAFLD through activation of FXR in mice.; Authors - Ming Gu
rat	WB	CNS Neurosci Ther; Endoplasmic reticulum stress induces apoptosis of arginine vasopressin neurons in central diabetes insipidus via PI3K/Akt pathway.; Authors - Ming-Feng Zhou
rat	WB	Br J Pharmacol; Hydrogen Sulfide Regulates Muscle RING Finger-1 Protein S-Sulfhydration at Cys44 to Prevent Cardiac Structural Damage in Diabetic Cardiomyopathy.; Authors - Xiaojiao Sun
human	WB	J Virol; Quantitative proteomic analysis reveals unfolded protein response involved in severe fever with thrombocytopenia syndrome virus infection.; Authors - Lei-Ke Zhang ; KD Validated
rat	WB	Int J Mol Med; Protective effect of Barbaloin in a rat model of myocardial ischemia reperfusion injury through the regulation of the CNPY2‑PERK pathway.; Authors - Yue Cui
mouse	wb	Cells; PMID: 31159306; Authors - Takayuki Inoue

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Maria (Verified Customer) (02-10-2021)

PERK in human primary fibroblasts. 12% TGX gel. Blocking 1h RT 3% BSA in PBST (0.1% Tween). Primary incubation 16h 4ºC 1:1000. Secondary 1:5000 Goat anti-rabbit HRP 1h RT.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human Primary Fibroblasts

PERK/EIF2AK3 Antibody Western Blot validation (1:1000 dilution) in Human Primary Fibroblasts (Cat no:20582-1-AP)

FH

Aeid (Verified Customer) (11-12-2018)

Works fine for HEK293 but not for MEF or INS1 cells

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293, MEF, INS1