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  • KD/KO Validated

PERK/EIF2AK3 Polyclonal antibody

PERK/EIF2AK3 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 20582-1-AP

Synonyms

EIF2AK3, HRI, HsPEK, Pancreatic eIF2 alpha kinase, PEK, PERK, PERK/EIF2AK3, WRS



Tested Applications

Positive WB detected inHEK-293 cells, mouse pancreas tissue, HepG2 cells, MCF-7 cells
Positive IHC detected inhuman pancreas tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

20582-1-AP targets PERK/EIF2AK3 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity bovine, human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name eukaryotic translation initiation factor 2-alpha kinase 3
Calculated molecular weight 125 kDa
Observed molecular weight 140 kDa
GenBank accession numberNM_004836
Gene symbol EIF2AK3
Gene ID (NCBI) 9451
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PERK, also named as EIF2AK3 or PEK, belongs to the protein kinase superfamily, Ser/Thr protein kinase family and GCN2 subfamily. It acts potentially as a metabolic sensor in the INS-secreting beta-cells to modulate the trafficking and quality control of pro-INS in the ER relative to the physiological demands for circulating INS (PMID:20530744). PERK and EIF2AK4 also have a functional role on regulating translation under non-stressed conditions, apart from their long established roles as stress kinases (PMID: 21638082). Defects in EIF2AK3 are the cause of Wolcott-Rallison syndrome (WRS). The molecular mass of PERK is 125 kDa and can be detected as 140 kDa or 170kDa due to some modifications such as glycosylation and phosphorylation (PMID: 20851775, 24371088, 21703327). This antibody is specific to PERK.

Protocols

Product Specific Protocols
WB protocol for PERK/EIF2AK3 antibody 20582-1-APDownload protocol
IHC protocol for PERK/EIF2AK3 antibody 20582-1-APDownload protocol
IF protocol for PERK/EIF2AK3 antibody 20582-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB

Br J Pharmacol

Betulinic acid alleviates endoplasmic reticulum stress-mediated NAFLD through activation of FXR in mice.

Authors - Ming Gu
ratWB

CNS Neurosci Ther

Endoplasmic reticulum stress induces apoptosis of arginine vasopressin neurons in central diabetes insipidus via PI3K/Akt pathway.

Authors - Ming-Feng Zhou
ratWB

Br J Pharmacol

Hydrogen Sulfide Regulates Muscle RING Finger-1 Protein S-Sulfhydration at Cys44 to Prevent Cardiac Structural Damage in Diabetic Cardiomyopathy.

Authors - Xiaojiao Sun
humanWB

J Virol

Quantitative proteomic analysis reveals unfolded protein response involved in severe fever with thrombocytopenia syndrome virus infection.

Authors - Lei-Ke Zhang
  • KD Validated
ratWB

Int J Mol Med

Protective effect of Barbaloin in a rat model of myocardial ischemia reperfusion injury through the regulation of the CNPY2‑PERK pathway.

Authors - Yue Cui
mousewb

Cells

PMID: 31159306

Authors - Takayuki Inoue

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Maria (Verified Customer) (02-10-2021)

PERK in human primary fibroblasts. 12% TGX gel. Blocking 1h RT 3% BSA in PBST (0.1% Tween). Primary incubation 16h 4ºC 1:1000. Secondary 1:5000 Goat anti-rabbit HRP 1h RT.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Human Primary Fibroblasts
PERK/EIF2AK3 Antibody Western Blot validation (1:1000 dilution) in Human Primary Fibroblasts (Cat no:20582-1-AP)
FH

Aeid (Verified Customer) (11-12-2018)

Works fine for HEK293 but not for MEF or INS1 cells

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HEK293, MEF, INS1