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PFKM Polyclonal antibody

PFKM Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat and More (3)





Cat no : 55028-1-AP


GSD7, PFK 1, PFK A, PFK M, PFKM, PFKX, Phosphofructokinase 1, Phosphofructokinase M, phosphofructokinase, muscle, Phosphohexokinase

Tested Applications

Positive WB detected inHeLa cells, human skeletal muscle tissue, HepG2 cells, human brain tissue, HEK-293 cells, mouse heart tissue, rat heart tissue
Positive IP detected inmouse heart tissue, mouse liver tissue
Positive IHC detected inmouse skeletal muscle tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:2000-1:16000 for WB
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

55028-1-AP targets PFKM in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, chicken, pig, bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name phosphofructokinase, muscle
Calculated molecular weight 85 kDa
Observed molecular weight 75-85 kDa
GenBank accession numberNM_000289
Gene symbol PFKM
Gene ID (NCBI) 5213
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PFKM, also named as GSD7, PFK-1, PFK-M and PFKX, belongs to the phosphofructokinase family and Two domains subfamily. PFKM catalyze the reaction: ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate. It is a key regulatory enzyme in glycolysis. Defects in PFKM are the cause of glycogen storage disease type 7 (GSD7). PFKM has three isoforms with molecular mass of 85, 82 and 93 kDa. The antibody is specific to PFKM.


Product Specific Protocols
WB protocol for PFKM antibody 55028-1-APDownload protocol
IHC protocol for PFKM antibody 55028-1-APDownload protocol
IF protocol for PFKM antibody 55028-1-APDownload protocol
IP protocol for PFKM antibody 55028-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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