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PFKM Polyclonal antibody

PFKM Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 55028-1-AP

Synonyms

GSD7, PFK 1, PFK A, PFK M, PFKM, PFKX, Phosphofructokinase 1, Phosphofructokinase M, phosphofructokinase, muscle, Phosphohexokinase



Tested Applications

Positive WB detected inhuman skeletal muscle tissue, HeLa cells, HepG2 cells, human brain tissue
Positive IP detected inmouse skeletal muscle tissue, mouse liver tissue
Positive IHC detected inhuman skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:3000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

55028-1-AP targets PFKM in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, pig, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name phosphofructokinase, muscle
Calculated molecular weight 85 kDa
Observed molecular weight 75-85 kDa
GenBank accession numberNM_000289
Gene symbol PFKM
Gene ID (NCBI) 5213
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer 0.1M NaHCO3, 0.1M glycine, 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

PFKM, also named as GSD7, PFK-1, PFK-M and PFKX, belongs to the phosphofructokinase family and Two domains subfamily. PFKM catalyze the reaction: ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate. It is a key regulatory enzyme in glycolysis. Defects in PFKM are the cause of glycogen storage disease type 7 (GSD7). PFKM has three isoforms with molecular mass of 85, 82 and 93 kDa. The antibody is specific to PFKM.

Protocols

Product Specific Protocols
WB protocol for PFKM antibody 55028-1-APDownload protocol
IHC protocol for PFKM antibody 55028-1-APDownload protocol
IF protocol for PFKM antibody 55028-1-APDownload protocol
IP protocol for PFKM antibody 55028-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Am J Physiol Regul Integr Comp Physiol

Protein recycling and limb muscle recovery after critical illness in slow- and fast-twitch limb muscle.

Authors - Sebastien Preau
pigWB

Anat Cell Biol

Poly(ADP-ribose) polymerase regulates glycolytic activity in kidney proximal tubule epithelial cells.

Authors - Hana Song
mouseWB

Oxid Med Cell Longev

Mild Hypothermia Attenuates Hepatic Ischemia-Reperfusion Injury through Regulating the JAK2/STAT3-CPT1a-Dependent Fatty Acid β-Oxidation.

Authors - Wei Wang
mouseWB

Cell Death Dis

The role of S-nitrosylation of PFKM in regulation of glycolysis in ovarian cancer cells.

Authors - Wenwen Gao
humanWB

Evid Based Complement Alternat Med

PMID: 28785292

Authors - Li Wu
ratWB

Biochem Biophys Res Commun

PMID: 27207834

Authors - Kelei Dong