PFKM Polyclonal antibody

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  WB analysis using 55028-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55028-1-AP (PFKM antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 55028-1-AP

  WB result of PFKM antibody (55028-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PFKM transfected HeLa cells.

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  WB analysis of HEK-293 using 55028-1-AP

  WB result of PFKM antibody (55028-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PFKM transfected HEK-293 cells.

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  WB analysis using 55028-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 55028-1-AP (PFKM antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human skeletal muscle using 55028-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 55028-1-AP (PFKM antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 55028-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 55028-1-AP (PFKM antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 55028-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 55028-1-AP (PFKM antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 55028-1-AP

  IP result of anti-PFKM (IP:55028-1-AP, 4ug; Detection:55028-1-AP 1:8000) with mouse heart tissue lysate 1680 ug.

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  IP experiment of mouse liver using 55028-1-AP

  IP result of anti-PFKM (IP:55028-1-AP, 4ug; Detection:55028-1-AP 1:300) with mouse liver tissue lysate 5200ug.

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  IHC staining of human skeletal muscle using 55028-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 55028-1-AP (PFKM Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skeletal muscle using 55028-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 55028-1-AP (PFKM Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse skeletal muscle using 55028-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 55028-1-AP (PFKM antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 55028-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 55028-1-AP (PFKM antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 55028-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 55028-1-AP (PFKM antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 55028-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 55028-1-AP (PFKM antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 55028-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PFKM antibody (55028-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

• Featured Product
• KD/KO Validated

PFKM Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 55028-1-AP

Publications(80)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (4)

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

6-phosphofructokinase type A, ATP-dependent 6-phosphofructokinase, muscle type, ATP-PFK, EC:2.7.1.11, PFK A

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, HepG2 cells, human brain tissue, human skeletal muscle tissue, L02 cells, mouse heart tissue, rat heart tissue
• Positive IP detected in: mouse heart tissue, mouse liver tissue
• Positive IHC detected in: mouse skeletal muscle tissue, human skeletal muscle tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 74 publications below
• IHC: See 3 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Product Information

55028-1-AP targets PFKM in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, chicken, bovine, sheep
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: phosphofructokinase, muscle
• Calculated Molecular Weight: 85 kDa
• Observed Molecular Weight: 75-85 kDa
• GenBank Accession Number: NM_000289
• Gene Symbol: PFKM
• Gene ID (NCBI): 5213
• RRID: AB_10858390
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P08237
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PFKM, also named as GSD7, PFK-1, PFK-M and PFKX, belongs to the phosphofructokinase family and two domains subfamily. PFKM catalyzes the reaction: ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate. It is a key regulatory enzyme in glycolysis. Defects in PFKM are the cause of glycogen storage disease type 7 (GSD7). PFKM has three isoforms with molecular masses of 85, 82 and 93 kDa. The antibody is specific to PFKM.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Cell Res; Mannose antagonizes GSDME-mediated pyroptosis through AMPK activated by metabolite GlcNAc-6P; Authors - Yuan-Li Ai
human	WB	Nature; Mechanical regulation of glycolysis via cytoskeleton architecture.; Authors - Jin Suk Park
human	WB	Nat Methods; A large-scale targeted proteomics assay resource based on an in vitro human proteome.; Authors - Masaki Matsumoto
mouse	IF	Nat Neurosci; A glycolytic shift in Schwann cells supports injured axons.; Authors - Elisabetta Babetto
human	WB	Biomaterials; Liver-targeted Nano-MitoPBN normalizes glucose metabolism by improving mitochondrial redox balance.; Authors - Meiling Wu
human	WB	Cancer Res; Disrupting Circadian Rhythm via the PER1-HK2 Axis Reverses Trastuzumab Resistance in Gastric Cancer.; Authors - Jiao Wang