Validation Data Gallery
|Positive WB detected in||human skeletal muscle tissue, HeLa cells, HepG2 cells, human brain tissue|
|Positive IP detected in||mouse skeletal muscle tissue, mouse liver tissue|
|Positive IHC detected in||human skeletal muscle tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:500-1:3000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:3000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
55028-1-AP targets PFKM in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, pig, rat|
|Host / Isotype||Rabbit / IgG|
|Full Name||phosphofructokinase, muscle|
|Calculated molecular weight||85 kDa|
|Observed molecular weight||75-85 kDa|
|GenBank accession number||NM_000289|
|Gene ID (NCBI)||5213|
|Purification Method||Antigen affinity purification|
|Storage Buffer||0.1M NaHCO3, 0.1M glycine, 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Aliquoting is unnecessary for -20oC storage.|
PFKM, also named as GSD7, PFK-1, PFK-M and PFKX, belongs to the phosphofructokinase family and Two domains subfamily. PFKM catalyze the reaction: ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate. It is a key regulatory enzyme in glycolysis. Defects in PFKM are the cause of glycogen storage disease type 7 (GSD7). PFKM has three isoforms with molecular mass of 85, 82 and 93 kDa. The antibody is specific to PFKM.
|Product Specific Protocols|
|WB protocol for PFKM antibody 55028-1-AP||Download protocol|
|IHC protocol for PFKM antibody 55028-1-AP||Download protocol|
|IF protocol for PFKM antibody 55028-1-AP||Download protocol|
|IP protocol for PFKM antibody 55028-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Am J Physiol Regul Integr Comp Physiol
Protein recycling and limb muscle recovery after critical illness in slow- and fast-twitch limb muscle.
Anat Cell Biol
Poly(ADP-ribose) polymerase regulates glycolytic activity in kidney proximal tubule epithelial cells.
Oxid Med Cell Longev
Mild Hypothermia Attenuates Hepatic Ischemia-Reperfusion Injury through Regulating the JAK2/STAT3-CPT1a-Dependent Fatty Acid β-Oxidation.
Cell Death Dis
The role of S-nitrosylation of PFKM in regulation of glycolysis in ovarian cancer cells.
Evid Based Complement Alternat Med
Biochem Biophys Res Commun