Validation Data Gallery
|Positive WB detected in||HeLa cells, human skeletal muscle tissue, HepG2 cells, human brain tissue, HEK-293 cells, mouse heart tissue, rat heart tissue|
|Positive IP detected in||mouse heart tissue, mouse liver tissue|
|Positive IHC detected in||mouse skeletal muscle tissue, human skeletal muscle tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:2000-1:16000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:2000-1:16000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:400-1:1600|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
55028-1-AP targets PFKM in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, chicken, pig, bovine|
|Host / Isotype||Rabbit / IgG|
|Full Name||phosphofructokinase, muscle|
|Calculated molecular weight||85 kDa|
|Observed molecular weight||75-85 kDa|
|GenBank accession number||NM_000289|
|Gene ID (NCBI)||5213|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
PFKM, also named as GSD7, PFK-1, PFK-M and PFKX, belongs to the phosphofructokinase family and Two domains subfamily. PFKM catalyze the reaction: ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate. It is a key regulatory enzyme in glycolysis. Defects in PFKM are the cause of glycogen storage disease type 7 (GSD7). PFKM has three isoforms with molecular mass of 85, 82 and 93 kDa. The antibody is specific to PFKM.
|Product Specific Protocols|
|WB protocol for PFKM antibody 55028-1-AP||Download protocol|
|IHC protocol for PFKM antibody 55028-1-AP||Download protocol|
|IF protocol for PFKM antibody 55028-1-AP||Download protocol|
|IP protocol for PFKM antibody 55028-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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