Tested Applications
| Positive WB detected in | HeLa cells, HEK-293 cells, HepG2 cells, human brain tissue, human skeletal muscle tissue, L02 cells, mouse heart tissue, rat heart tissue |
| Positive IP detected in | mouse liver tissue |
| Positive IHC detected in | mouse skeletal muscle tissue, human skeletal muscle tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
55028-1-AP targets PFKM in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig, chicken, bovine, sheep |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | phosphofructokinase, muscle |
| Calculated Molecular Weight | 85 kDa |
| Observed Molecular Weight | 75-85 kDa |
| GenBank Accession Number | NM_000289 |
| Gene Symbol | PFKM |
| Gene ID (NCBI) | 5213 |
| RRID | AB_10858390 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P08237 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PFKM, also named as GSD7, PFK-1, PFK-M and PFKX, belongs to the phosphofructokinase family and two domains subfamily. PFKM catalyzes the reaction: ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate. It is a key regulatory enzyme in glycolysis. Defects in PFKM are the cause of glycogen storage disease type 7 (GSD7). PFKM has three isoforms with molecular masses of 85, 82 and 93 kDa. The antibody is specific to PFKM.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for PFKM antibody 55028-1-AP | Download protocol |
| IHC protocol for PFKM antibody 55028-1-AP | Download protocol |
| IP protocol for PFKM antibody 55028-1-AP | Download protocol |
| WB protocol for PFKM antibody 55028-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The PFKM Polyclonal antibody (Cat# 55028-1-AP) has 126 citations. These appear in high-impact journals including Nature, Cell Metabolism, Cell Research, Nature Methods, Nature Cell Biology, Nature Communications, Cancer Research, Cell Death & Disease, Redox Biology, Science Advances, and Free Radical Biology and Medicine. Research fields center on glycolysis and glucose metabolism, with major themes in cancer metabolic reprogramming, ischemic stroke and neurodegeneration, fibrosis, diabetes, mitochondrial function, and lactate-mediated signaling across human, mouse, rat, and other species.
| Species | Application | Title |
|---|---|---|
Cell Metab The diapause-like colorectal cancer cells induced by SMC4 attenuation are characterized by low proliferation and chemotherapy insensitivity | ||
Cell Metab Astrocytic LRP1 enables mitochondria transfer to neurons and mitigates brain ischemic stroke by suppressing ARF1 lactylation | ||
Cell Res Mannose antagonizes GSDME-mediated pyroptosis through AMPK activated by metabolite GlcNAc-6P | ||
Nat Methods A large-scale targeted proteomics assay resource based on an in vitro human proteome. | ||
