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PFKP Polyclonal antibody

PFKP Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 13389-1-AP

Synonyms

6 phosphofructokinase type C, PFK C, PFKF, PFKP, Phosphofructokinase 1, phosphofructokinase, platelet, Phosphohexokinase



Tested Applications

Positive WB detected inmouse heart tissue, BxPC-3 cells, HEK-293 cells, HeLa cells, human heart tissue, Jurkat cells, mouse skeletal muscle tissue
Positive IP detected inmouse heart tissue, Jurkat cells
Positive IHC detected inhuman spleen tissue, human heart tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inBxPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

13389-1-AP targets PFKP in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PFKP fusion protein Ag4047
Full Name phosphofructokinase, platelet
Calculated molecular weight 784 aa, 86 kDa
Observed molecular weight 86 kDa
GenBank accession numberBC029138
Gene symbol PFKP
Gene ID (NCBI) 5214
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PFKP(6-phosphofructokinase, platelet type) is also named as PFKF,PFK-C and belongs to the phosphofructokinase family.This form of PFK is best called the 'platelet' type and symbolized PFKP because it is the only form made by platelets, whereas fibroblasts have more than one form of PFK(Francke (1983)).PFK catalyzes the irreversible conversion of fructose-6-phosphate to fructose-1,6-bisphosphate and is a key regulatory enzyme in glycolysis.

Protocols

Product Specific Protocols
WB protocol for PFKP antibody 13389-1-APDownload protocol
IHC protocol for PFKP antibody 13389-1-APDownload protocol
IF protocol for PFKP antibody 13389-1-APDownload protocol
IP protocol for PFKP antibody 13389-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

J Cell Mol Med

Novel mitochondrion-targeting copper(II) complex induces HK2 malfunction and inhibits glycolysis via Drp1-mediating mitophagy in HCC.

Authors - Mengmeng Li
mouseWB

Cell Death Dis

The role of S-nitrosylation of PFKM in regulation of glycolysis in ovarian cancer cells.

Authors - Wenwen Gao
mouseWB

Acta Pharmacol Sin

Bmal1 inhibits phenotypic transformation of hepatic stellate cells in liver fibrosis via IDH1/α-KG-mediated glycolysis.

Authors - Lei Xu
mouseWB

Oncol Lett

Downregulation of GLUT3 impairs STYK1/NOK-mediated metabolic reprogramming and proliferation in NIH-3T3 cells.

Authors - Weiye Shi
mouseWB

Toxicol Appl Pharmacol

Triptolide impairs glycolysis by suppressing GATA4/Sp1/PFKP signaling axis in mouse Sertoli cells.

Authors - Yunhui Zhang
mouseWB

Front Pharmacol

Combination of the Herbs Radix Rehmanniae and Cornus Officinalis Mitigated Testicular Damage From Diabetes Mellitus by Enhancing Glycolysis via the AGEs/RAGE/HIF-1α Axis.

Authors - Yuping Chen