Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells, BxPC-3 cells, human heart tissue, NIH/3T3 cells, mouse heart tissue, mouse skeletal muscle tissue, rat heart tissue, rat skeletal muscle tissue, Jurkat cells, MCF-7 cells, Raji cells |
| Positive IP detected in | Jurkat cells, mouse heart tissue |
| Positive IHC detected in | mouse heart tissue, human lung cancer tissue, human heart tissue, human spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells, BxPC-3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 30 publications below |
| IHC | See 4 publications below |
| IF | See 3 publications below |
| IP | See 2 publications below |
Product Information
13389-1-AP targets PFKP in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | PFKP fusion protein Ag4047 Predict reactive species |
| Full Name | phosphofructokinase, platelet |
| Calculated Molecular Weight | 784 aa, 86 kDa |
| Observed Molecular Weight | 80-86 kDa |
| GenBank Accession Number | BC029138 |
| Gene Symbol | PFKP |
| Gene ID (NCBI) | 5214 |
| RRID | AB_2252278 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q01813 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PFKP(6-phosphofructokinase, platelet type) is also named as PFKF, PFK-C and belongs to the phosphofructokinase family. This form of PFK is best called the 'platelet' type and symbolized PFKP because it is the only form made by platelets, whereas fibroblasts have more than one form of PFK (Francke (1983)). PFK catalyzes the irreversible conversion of fructose-6-phosphate to fructose-1,6-bisphosphate and is a key regulatory enzyme in glycolysis. The observed molecular weight of PFKP monomer is 80-86 kDa. The molecular mass of PFKP dimer is 171 kDa (PMID: 28607489).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PFKP antibody 13389-1-AP | Download protocol |
| IHC protocol for PFKP antibody 13389-1-AP | Download protocol |
| IF protocol for PFKP antibody 13389-1-AP | Download protocol |
| IP protocol for PFKP antibody 13389-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Res Mannose antagonizes GSDME-mediated pyroptosis through AMPK activated by metabolite GlcNAc-6P | ||
Cell Metab Augmentation of scleral glycolysis promotes myopia through histone lactylation | ||
Mol Cell R-2-hydroxyglutarate attenuates aerobic glycolysis in leukemia by targeting the FTO/m6A/PFKP/LDHB axis.
| ||
Stem Cell Reports The Long Noncoding RNA Lncenc1 Maintains Naive States of Mouse ESCs by Promoting the Glycolysis Pathway. | ||
Acta Pharmacol Sin Bmal1 inhibits phenotypic transformation of hepatic stellate cells in liver fibrosis via IDH1/α-KG-mediated glycolysis. |
