PFKP Polyclonal antibody

• Featured Product
• KD/KO Validated

PFKP Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

13

Cat no : 13389-1-AP

Synonyms

6 phosphofructokinase type C, PFK C, PFKF, PFKP, Phosphofructokinase 1, phosphofructokinase, platelet, Phosphohexokinase

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Validation Data Gallery

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  WB analysis using 13389-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13389-1-AP (PFKP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 13389-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 13389-1-AP (PFKP Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 13389-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 13389-1-AP (PFKP antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 13389-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 13389-1-AP (PFKP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 13389-1-AP

  IP Result of anti-PFKP (IP:13389-1-AP, 4ug; Detection:13389-1-AP 1:1000) with mouse heart tissue lysate 4000ug.

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  IP experiment of Jurkat using 13389-1-AP

  IP Result of anti-PFKP (IP:13389-1-AP, 4ug; Detection:13389-1-AP 1:1000) with Jurkat cells lysate 3400ug.

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  IHC staining of human spleen using 13389-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 13389-1-AP (PFKP Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human spleen using 13389-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 13389-1-AP (PFKP Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human heart using 13389-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 13389-1-AP (PFKP Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 13389-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 13389-1-AP (PFKP Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung cancer using 13389-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13389-1-AP (PFKP Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lung cancer using 13389-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13389-1-AP (PFKP Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of BxPC-3 using 13389-1-AP

  Immunofluorescent analysis of BxPC-3 cells using 13389-1-AP (PFKP antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: BxPC-3 cells, mouse skeletal muscle tissue, mouse heart tissue, human heart tissue, HEK-293 cells, HeLa cells, Jurkat cells
• Positive IP detected in: mouse heart tissue, Jurkat cells
• Positive IHC detected in: human spleen tissue, human heart tissue, human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: BxPC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:10-1:100

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 12 publications below
• IHC: See 3 publications below

Product Information

13389-1-AP targets PFKP in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PFKP fusion protein Ag4047
• Full Name: phosphofructokinase, platelet
• Calculated molecular weight: 784 aa, 86 kDa
• Observed molecular weight: 86 kDa
• GenBank accession number: BC029138
• Gene symbol: PFKP
• Gene ID (NCBI): 5214
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

PFKP(6-phosphofructokinase, platelet type) is also named as PFKF,PFK-C and belongs to the phosphofructokinase family.This form of PFK is best called the 'platelet' type and symbolized PFKP because it is the only form made by platelets, whereas fibroblasts have more than one form of PFK(Francke (1983)).PFK catalyzes the irreversible conversion of fructose-6-phosphate to fructose-1,6-bisphosphate and is a key regulatory enzyme in glycolysis.

Publications

Species	Application	Title
human	WB,IHC	J Cell Mol Med; Novel mitochondrion-targeting copper(II) complex induces HK2 malfunction and inhibits glycolysis via Drp1-mediating mitophagy in HCC.; Authors - Mengmeng Li
mouse	WB	Cell Death Dis; The role of S-nitrosylation of PFKM in regulation of glycolysis in ovarian cancer cells.; Authors - Wenwen Gao
mouse	WB	Acta Pharmacol Sin; Bmal1 inhibits phenotypic transformation of hepatic stellate cells in liver fibrosis via IDH1/α-KG-mediated glycolysis.; Authors - Lei Xu
mouse	WB	Oncol Lett; Downregulation of GLUT3 impairs STYK1/NOK-mediated metabolic reprogramming and proliferation in NIH-3T3 cells.; Authors - Weiye Shi
mouse	WB	Toxicol Appl Pharmacol; Triptolide impairs glycolysis by suppressing GATA4/Sp1/PFKP signaling axis in mouse Sertoli cells.; Authors - Yunhui Zhang
mouse	WB	Front Pharmacol; Combination of the Herbs Radix Rehmanniae and Cornus Officinalis Mitigated Testicular Damage From Diabetes Mellitus by Enhancing Glycolysis via the AGEs/RAGE/HIF-1α Axis.; Authors - Yuping Chen