PFKP Polyclonal antibody

• Featured Product
• KD/KO Validated

PFKP Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Publications

9

Cat no : 13389-1-AP

Synonyms

6 phosphofructokinase type C, PFK C, PFKF, PFKP, Phosphofructokinase 1, phosphofructokinase, platelet, Phosphohexokinase

---

Validation Data Gallery

• 

  WB analysis of mouse heart using 13389-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 13389-1-AP (PFKP antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse heart using 13389-1-AP

  IP Result of anti-PFKP (IP:13389-1-AP, 4ug; Detection:13389-1-AP 1:1000) with mouse heart tissue lysate 4000ug.

• 

  IHC staining of human spleen using 13389-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 13389-1-AP (PFKP Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human spleen using 13389-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 13389-1-AP (PFKP Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of BxPC-3 using 13389-1-AP

  Immunofluorescent analysis of BxPC-3 cells using 13389-1-AP (PFKP antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive WB detected in: mouse heart tissue, BxPC-3 cells, HEK-293 cells, HeLa cells, human heart tissue, Jurkat cells, mouse skeletal muscle tissue
• Positive IP detected in: mouse heart tissue, Jurkat cells
• Positive IHC detected in: human spleen tissue, human heart tissue, human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: BxPC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:10-1:100

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 2 publications below
• WB: See 8 publications below
• IHC: See 3 publications below

Product Information

13389-1-AP targets PFKP in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PFKP fusion protein Ag4047
• Full Name: phosphofructokinase, platelet
• Calculated molecular weight: 784 aa, 86 kDa
• Observed molecular weight: 86 kDa
• GenBank accession number: BC029138
• Gene symbol: PFKP
• Gene ID (NCBI): 5214
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

PFKP(6-phosphofructokinase, platelet type) is also named as PFKF,PFK-C and belongs to the phosphofructokinase family.This form of PFK is best called the 'platelet' type and symbolized PFKP because it is the only form made by platelets, whereas fibroblasts have more than one form of PFK(Francke (1983)).PFK catalyzes the irreversible conversion of fructose-6-phosphate to fructose-1,6-bisphosphate and is a key regulatory enzyme in glycolysis.

Publications

Species	Application	Title
human	WB,IHC	J Cell Mol Med; Novel mitochondrion-targeting copper(II) complex induces HK2 malfunction and inhibits glycolysis via Drp1-mediating mitophagy in HCC.; Authors - Mengmeng Li
mouse	WB	Cell Death Dis; The role of S-nitrosylation of PFKM in regulation of glycolysis in ovarian cancer cells.; Authors - Wenwen Gao
mouse	WB	Acta Pharmacol Sin; Bmal1 inhibits phenotypic transformation of hepatic stellate cells in liver fibrosis via IDH1/α-KG-mediated glycolysis.; Authors - Lei Xu
mouse	WB	Front Pharmacol; Bmal1 Regulates Macrophage Polarize Through Glycolytic Pathway in Alcoholic Liver Disease.; Authors - Yiwen Zhou
mouse	WB	Biochim Biophys Acta Mol Basis Dis; A long non-coding RNA specifically expressed in early embryos programs the metabolic balance in adult mice.; Authors - Minzhe Zhu
human	WB,IHC	Biochem Pharmacol; Ginsenoside Rh4 suppresses aerobic glycolysis and the expression of PD-L1 via targeting AKT in esophageal cancer.; Authors - Xuqian Deng