PFKP Polyclonal antibody

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  WB analysis using 13389-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13389-1-AP (PFKP antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis using 13389-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13389-1-AP (PFKP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13389-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13389-1-AP (PFKP antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 13389-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 13389-1-AP (PFKP antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 13389-1-AP

  IP result of anti-PFKP (IP:13389-1-AP, 4ug; Detection:13389-1-AP 1:4000) with Jurkat cells lysate 2280 ug.

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  IP experiment of mouse heart using 13389-1-AP

  IP result of anti-PFKP (IP:13389-1-AP, 4ug; Detection:13389-1-AP 1:1000) with mouse heart tissue lysate 4000ug.

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  IHC staining of mouse heart using 13389-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 13389-1-AP (PFKP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 13389-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 13389-1-AP (PFKP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 13389-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13389-1-AP (PFKP antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 13389-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13389-1-AP (PFKP antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human spleen using 13389-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 13389-1-AP (PFKP Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human spleen using 13389-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 13389-1-AP (PFKP Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 13389-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 13389-1-AP (PFKP Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 13389-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 13389-1-AP (PFKP Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 13389-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using PFKP antibody (13389-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of BxPC-3 using 13389-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed BxPC-3 cells using PFKP antibody (13389-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

PFKP Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 13389-1-AP

Publications(34)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

PFKF, PFK-C, EC:2.7.1.11, ATP-PFK, ATP-dependent 6-phosphofructokinase, platelet type

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, BxPC-3 cells, human heart tissue, NIH/3T3 cells, mouse heart tissue, mouse skeletal muscle tissue, rat heart tissue, rat skeletal muscle tissue, Jurkat cells, MCF-7 cells, Raji cells
• Positive IP detected in: Jurkat cells, mouse heart tissue
• Positive IHC detected in: mouse heart tissue, human lung cancer tissue, human heart tissue, human spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells, BxPC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 30 publications below
• IHC: See 4 publications below
• IF: See 3 publications below
• IP: See 2 publications below

Product Information

13389-1-AP targets PFKP in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PFKP fusion protein Ag4047
• Full Name: phosphofructokinase, platelet
• Calculated Molecular Weight: 784 aa, 86 kDa
• Observed Molecular Weight: 80-86 kDa
• GenBank Accession Number: BC029138
• Gene Symbol: PFKP
• Gene ID (NCBI): 5214
• RRID: AB_2252278
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q01813
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PFKP(6-phosphofructokinase, platelet type) is also named as PFKF, PFK-C and belongs to the phosphofructokinase family. This form of PFK is best called the 'platelet' type and symbolized PFKP because it is the only form made by platelets, whereas fibroblasts have more than one form of PFK (Francke (1983)). PFK catalyzes the irreversible conversion of fructose-6-phosphate to fructose-1,6-bisphosphate and is a key regulatory enzyme in glycolysis. The observed molecular weight of PFKP monomer is 80-86 kDa. The molecular mass of PFKP dimer is 171 kDa (PMID: 28607489).

Publications

Species	Application	Title
human	WB	Cell Res; Mannose antagonizes GSDME-mediated pyroptosis through AMPK activated by metabolite GlcNAc-6P; Authors - Yuan-Li Ai
mouse	WB	Cell Metab; Augmentation of scleral glycolysis promotes myopia through histone lactylation; Authors - Xiaolei Lin
mouse	WB,IF	Nat Metab; Time of exercise differentially impacts bone growth in mice; Authors - Shaoling Yu
human	WB	Mol Cell; R-2-hydroxyglutarate attenuates aerobic glycolysis in leukemia by targeting the FTO/m6A/PFKP/LDHB axis.; Authors - Ying Qing ; KD Validated
human	WB	Stem Cell Reports; The Long Noncoding RNA Lncenc1 Maintains Naive States of Mouse ESCs by Promoting the Glycolysis Pathway.; Authors - Zihao Sun
mouse	WB	Acta Pharmacol Sin; Bmal1 inhibits phenotypic transformation of hepatic stellate cells in liver fibrosis via IDH1/α-KG-mediated glycolysis.; Authors - Lei Xu