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- KD/KO Validated
PFKP Polyclonal antibody
PFKP Polyclonal Antibody for IF, IHC, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, IF,ELISA
Conjugate
Unconjugated
Cat no : 13389-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse heart tissue, BxPC-3 cells, HEK-293 cells, HeLa cells, human heart tissue, Jurkat cells, mouse skeletal muscle tissue |
| Positive IP detected in | mouse heart tissue, Jurkat cells |
| Positive IHC detected in | human spleen tissue, human heart tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | BxPC-3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF) | IF : 1:10-1:100 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 11 publications below |
| IHC | See 3 publications below |
Product Information
13389-1-AP targets PFKP in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | PFKP fusion protein Ag4047 |
| Full Name | phosphofructokinase, platelet |
| Calculated molecular weight | 784 aa, 86 kDa |
| Observed molecular weight | 86 kDa |
| GenBank accession number | BC029138 |
| Gene symbol | PFKP |
| Gene ID (NCBI) | 5214 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PFKP(6-phosphofructokinase, platelet type) is also named as PFKF,PFK-C and belongs to the phosphofructokinase family.This form of PFK is best called the 'platelet' type and symbolized PFKP because it is the only form made by platelets, whereas fibroblasts have more than one form of PFK(Francke (1983)).PFK catalyzes the irreversible conversion of fructose-6-phosphate to fructose-1,6-bisphosphate and is a key regulatory enzyme in glycolysis.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PFKP antibody 13389-1-AP | Download protocol |
| IHC protocol for PFKP antibody 13389-1-AP | Download protocol |
| IF protocol for PFKP antibody 13389-1-AP | Download protocol |
| IP protocol for PFKP antibody 13389-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Cell Mol Med Novel mitochondrion-targeting copper(II) complex induces HK2 malfunction and inhibits glycolysis via Drp1-mediating mitophagy in HCC. | ||
Cell Death Dis The role of S-nitrosylation of PFKM in regulation of glycolysis in ovarian cancer cells. | ||
Acta Pharmacol Sin Bmal1 inhibits phenotypic transformation of hepatic stellate cells in liver fibrosis via IDH1/α-KG-mediated glycolysis. | ||
Oncol Lett Downregulation of GLUT3 impairs STYK1/NOK-mediated metabolic reprogramming and proliferation in NIH-3T3 cells. | ||
Toxicol Appl Pharmacol Triptolide impairs glycolysis by suppressing GATA4/Sp1/PFKP signaling axis in mouse Sertoli cells. | ||
Front Pharmacol Combination of the Herbs Radix Rehmanniae and Cornus Officinalis Mitigated Testicular Damage From Diabetes Mellitus by Enhancing Glycolysis via the AGEs/RAGE/HIF-1α Axis. |
