PGAP1 Polyclonal antibody

PGAP1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 55392-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Bst1, GPI inositol deacylase, hPGAP1, ISPD3024, PGAP1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

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Tested Applications

Positive WB detected inhuman testis tissue, HEK-293 cells, MCF-7 cells, mouse testis tissue
Positive IHC detected inhuman testis tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55392-1-AP targets PGAP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide Predict reactive species
Full Name post-GPI attachment to proteins 1
Calculated Molecular Weight 105 kDa
Observed Molecular Weight 49-53 kDa, 85-90 kDa
GenBank Accession NumberNM_024989
Gene Symbol PGAP1
Gene ID (NCBI) 80055
RRIDAB_11232412
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ75T13
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PGAP1 belongs to the GPI inositol-deacylase family. It is involved in inositol deacylation of GPI-anchored proteins. GPI inositol deacylation may important for efficient transport of GPI-anchored proteins from the endoplasmic reticulum to the Golgi. PGAP1 is the GPI inositol-deacylase (PMID:14734546). This antibody is specific to PGAP1.

Protocols

Product Specific Protocols
WB protocol for PGAP1 antibody 55392-1-APDownload protocol
IHC protocol for PGAP1 antibody 55392-1-APDownload protocol
IF protocol for PGAP1 antibody 55392-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
HumanIHC

Nature

A single-cell atlas of human and mouse white adipose tissue.

Authors - Margo P Emont
humanWB

Mol Cell

Integrative omics indicate FMRP sequesters mRNA from translation and deadenylation in human neuronal cells

Authors - Tatsuaki Kurosaki
humanWB

Cancer Med

Identification of novel pQTL-SNPs associated with lung adenocarcinoma risk: A multi-stage study

Authors - Yutong Wu
humanWB

J Biol Chem

Identification of mammalian glycoproteins with type-I LacdiNAc structures synthesized by the glycosyltransferase B3GALNT2.

Authors - Takahiro Nakane

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

pitna (Verified Customer) (12-19-2019)

PGAP1According to Uniprot, this protein has 4 isoforms ranging from 49 – 105 KDa. it appears that the regulation of the different isoforms is still an open question in the literatureTo reduce non-specific binding, blots were incubated in Odyssey Blocking buffer (Li-Cor) for 1 hour at room temperature (RT) .

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1,000
  • Cell Tissue Type: Postmortem human brain : dorsolateral prefrontal cortex (DLPFC)
PGAP1 Antibody Western Blot, validation (1:1,000 dilution) in Postmortem human brain : dorsolateral prefrontal cortex (DLPFC) (Cat no:55392-1-AP)
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