PGD Polyclonal antibody

• Featured Product
• KD/KO Validated

PGD Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

20

Cat no : 14718-1-AP

Synonyms

PGDH, EC:1.1.1.44, 6-phosphogluconate dehydrogenase, decarboxylating, 6PGD

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Validation Data Gallery

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  WB analysis using 14718-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14718-1-AP (PGD antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 14718-1-AP

  IP result of anti-PGD (IP:14718-1-AP, 4ug; Detection:14718-1-AP 1:4000) with HepG2 cells lysate 1925 ug.

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  IHC staining of human liver cancer using 14718-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14718-1-AP (PGD Antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 14718-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14718-1-AP (PGD Antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lymphoma using 14718-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 14718-1-AP (PGD Antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lymphoma using 14718-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 14718-1-AP (PGD Antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 14718-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14718-1-AP (PGD Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 14718-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14718-1-AP (PGD Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lymphoma using 14718-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 14718-1-AP (PGD Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lymphoma using 14718-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 14718-1-AP (PGD Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 14718-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PGD antibody (14718-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, Jurkat cells, mouse liver tissue, rat liver tissue
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human liver cancer tissue, human lymphoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:300-1:1200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 16 publications below
• IHC: See 6 publications below

Product Information

14718-1-AP targets PGD in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PGD fusion protein Ag6444
• Full Name: phosphogluconate dehydrogenase
• Calculated Molecular Weight: 53 kDa
• Observed Molecular Weight: 53 kDa, 45 kDa
• GenBank Accession Number: BC000368
• Gene Symbol: PGD
• Gene ID (NCBI): 5226
• RRID: AB_2236801
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PGD, also named as PGDH, belongs to the 6-phosphogluconate dehydrogenase family. PGD catalyses the oxidative decarboxylation of 6-phosphogluconate to ribulose 5-phosphate in the context of the oxidative part of the pentose phosphate pathway. PGD is important for the production of NADPH, which is necessary for reductive biosynthesis, such as the formation of lipids and nucleotides, and the activity of enzymes involved in maintaining cell integrity, in combatting oxidative stress and in the first line of immunological defence. (PMID: 35234135)

Publications

Species	Application	Title
human	WB	Nat Commun; Polo-like kinase 1 coordinates biosynthesis during cell cycle progression by directly activating pentose phosphate pathway.; Authors - Xiaoyu Ma
mouse	WB	Clin Transl Med; SIRT5 deficiency enhances the proliferative and therapeutic capacities of adipose-derived mesenchymal stem cells via metabolic switching.; Authors - Tiantong Ou
human	IHC	J Invest Dermatol; Enhanced Glycogen Metabolism Supports the Survival and Proliferation of HPV-Infected Keratinocytes in Condylomata Acuminata.; Authors - Zhichao Gu
human	WB	Biochem Pharmacol; PMID: 32535103; Authors - Qi Feng
mouse	WB	iScience; The Symbiotic Relationship between the Neural Retina and Retinal Pigment Epithelium Is Supported by Utilizing Differential Metabolic Pathways.; Authors - Tirthankar Sinha
human	WB,IHC	Carcinogenesis; YTH domain family 2 promotes lung cancer cell growth by facilitating 6-phosphogluconate dehydrogenase mRNA translation.; Authors - Hao Sheng ; KD Validated