Placental Growth Factor Polyclonal antibody

• Featured Product
• KD/KO Validated

Placental Growth Factor Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

20

Cat no : 10642-1-AP

Synonyms

D12S1900, PGF, PGFL, PIGF, Placenta growth factor, placental growth factor, PLGF, PlGF 2, SHGC 10760

---

Validation Data Gallery

• 

  WB analysis using 10642-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10642-1-AP (Placental Growth Factor antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10642-1-AP

  mouse placenta tissue were subjected to SDS PAGE followed by western blot with 10642-1-AP (Placental Growth Factor antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 10642-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 10642-1-AP (Placental Growth Factor antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human placenta using 10642-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10642-1-AP (Placental Growth Factor antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 10642-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10642-1-AP (Placental Growth Factor antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 10642-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10642-1-AP (Placental Growth Factor antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 10642-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10642-1-AP (Placental Growth Factor antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10642-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10642-1-AP (Placental Growth Factor antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10642-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10642-1-AP (Placental Growth Factor antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human meningioma using 10642-1-AP

  Immunohistochemical analysis of paraffin-embedded human meningioma using 10642-1-AP (Placental Growth Factor antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human meningioma using 10642-1-AP

  Immunohistochemical analysis of paraffin-embedded human meningioma using 10642-1-AP (Placental Growth Factor antibody) at dilution of 1:50 (under 40x lens).

---

Tested Applications

• Positive WB detected in: human placenta tissue, mouse placenta tissue
• Positive IHC detected in: human placenta tissue, human meningioma tissue, human kidney tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:200-1:800

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 3 publications below
• IHC: See 8 publications below
• IF: See 9 publications below

Product Information

10642-1-AP targets Placental Growth Factor in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Placental Growth Factor fusion protein Ag0969
• Full Name: placental growth factor
• Calculated molecular weight: 25 kDa
• Observed molecular weight: 50 kDa
• GenBank accession number: BC007789
• Gene symbol: PGF
• Gene ID (NCBI): 5228
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Placenta growth factor (PLGF) is a homodimeric glycoprotein, 46-50 kDa in size, belonging to the vascular endothelial growth factor (VEGF) sub-family. It plays an important role in pathological VEGF-driven angiogenesis. PLGF is expressed not only in placental cells but also in colon and mammary carcinomas. It acts as a primary inflammatory instigator of atherosclerotic plaque instability and thus may be useful as a risk-predicting biomarker in patients with acute coronary syndromes (ACS).

Publications

Species	Application	Title
rat	IF	Brain.; Global gene expression profiling of somatic motor neuron populations with different vulnerability identify molecules and pathways of degeneration and protection.; Authors - Hedlund E
mouse		Biomaterials; Combination antitumor immunotherapy with VEGF and PIGF siRNA via systemic delivery of multi-functionalized nanoparticles to tumor-associated macrophages and breast cancer cells.; Authors - Yudong Song ; KD Validated
mouse	IHC,IF	Biomed Pharmacother; HIF-1α inhibitor YC-1 suppresses triple-negative breast cancer growth and angiogenesis by targeting PlGF/VEGFR1-induced macrophage polarization; Authors - Yan Li
human	IHC	Cancers (Basel); Occurrence of Fibrotic Tumor Vessels in Grade I Meningiomas Is Strongly Associated with Vessel Density, Expression of VEGF, PlGF, IGFBP-3 and Tumor Recurrence.; Authors - Katharina Hess
mouse	IF	Diabetes Obes Metab; Polarized macrophages promote gestational beta cell growth through extracellular signal-regulated kinase 5 signalling.; Authors - Yinan Jiang
mouse	IF	Int J Mol Sci; Alzheimer's Amyloid β Peptide Induces Angiogenesis in an Alzheimer's Disease Model Mouse through Placental Growth Factor and Angiopoietin 2 Expressions; Authors - Abdullah Md Sheikh