Tested Applications
| Positive IHC detected in | human placenta tissue, human meningioma tissue, human kidney tissue, human breast cancer tissue, mouse kidney tissue, mouse placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human placenta tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10642-1-AP targets PGF/PLGF in IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0969 Product name: Recombinant human PGF protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-170 aa of BC007789 Sequence: MPVMRLFPCFLQLLAGLALPAVPPQQWALSAGNGSSEVEVVPFQEVWGRSYCRALERLVDVVSEYPSEVEHMFSPSCVSLLRCTGCCGDENLHCVPVETANVTMQLLKIRSGDRPSYVELTFSQHVRCECRPLREKMKPERRRPKGRGKRRREKQRPTDCHLCGDAVPRR Predict reactive species |
| Full Name | placental growth factor |
| Calculated Molecular Weight | 25 kDa |
| Observed Molecular Weight | 50 kDa |
| GenBank Accession Number | BC007789 |
| Gene Symbol | PGF |
| Gene ID (NCBI) | 5228 |
| RRID | AB_2161061 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P49763 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Placenta growth factor (PLGF) is also named as PGF and PGFL. PGF is a homodimeric glycoprotein, 46-50 kDa in size, belonging to the vascular endothelial growth factor (VEGF) sub-family. It plays an important role in pathological VEGF-driven angiogenesis. PLGF is expressed not only in placental cells but also in colon and mammary carcinomas. It acts as a primary inflammatory instigator of atherosclerotic plaque instability and thus may be useful as a risk-predicting biomarker in patients with acute coronary syndromes (ACS).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for PGF/PLGF antibody 10642-1-AP | Download protocol |
| IHC protocol for PGF/PLGF antibody 10642-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-PlGF antibody (Cat# 10642-1-AP) has accumulated 38 citations across journals such as Cancer Cell, Biomaterials, Brain, FASEB Journal, Stem Cell Research & Therapy, International Journal of Molecular Sciences, and European Journal of Pharmacology. Key research fields include oncology (breast, renal, hepatocellular, and bladder cancers), preeclampsia and placental biology, angiogenesis, neurodegeneration, cardiovascular disease, macrophage polarization, and fetal growth restriction.
| Species | Application | Title |
|---|---|---|
Biomaterials Combination antitumor immunotherapy with VEGF and PIGF siRNA via systemic delivery of multi-functionalized nanoparticles to tumor-associated macrophages and breast cancer cells.
| ||
Brain. Global gene expression profiling of somatic motor neuron populations with different vulnerability identify molecules and pathways of degeneration and protection. | ||
Int J Biol Sci Identification of a novel cancer stem cell subpopulation that promotes progression of human fatal renal cell carcinoma by single-cell RNA-seq analysis. | ||
Int J Biol Macromol Molecular mechanism of Flt-1 protein and the regulation of monocytes modulate endothelial cell in wound healing sites via PGF/FLT1 signaling | ||
Environ Res Prenatal triphenyl phosphate exposure impairs placentation and induces preeclampsia-like symptoms in mice |
