PGK1 Polyclonal antibody

• Featured Product
• KD/KO Validated

PGK1 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

27

Cat no : 17811-1-AP

Synonyms

MIG10, OK/SW cl.110, PGK1, PGKA, phosphoglycerate kinase 1, Primer recognition protein 2, PRP 2

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Validation Data Gallery

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  WB analysis of HeLa using 17811-1-AP

  WB result of PGK1 antibody (17811-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PGK1 transfected HeLa cells.

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  WB analysis of HeLa using 17811-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17811-1-AP (PGK1 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

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  IHC staining of human hepatocirrhosis using 17811-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 17811-1-AP (PGK1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human hepatocirrhosis using 17811-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 17811-1-AP (PGK1 Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: HeLa cells, mouse brain tissue, HEK-293 cells, A431 cells, rat liver tissue, HepG2 cells, mouse liver tissue, human stomach tissue, PC-3 cells
• Positive IHC detected in: human hepatocirrhosis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2400
• Immunohistochemistry (IHC): IHC : 1:20-1:200

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 4 publications below
• WB: See 22 publications below
• IHC: See 8 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

17811-1-AP targets PGK1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PGK1 fusion protein Ag12119
• Full Name: phosphoglycerate kinase 1
• Calculated molecular weight: 417 aa, 45 kDa
• Observed molecular weight: 40-45 kDa
• GenBank accession number: BC103752
• Gene symbol: PGK1
• Gene ID (NCBI): 5230
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

The PGK1 gene encodes phosphoglycerate kinase-1, also known as ATP:3-phosphoglycerate 1-phosphotransferase (EC 2.7.2.3), which catalyzes the reversible conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate during glycolysis, generating one molecule of ATP. It Belongs to the phosphoglycerate kinase family and defects in PGK1 are the cause of phosphoglycerate kinase 1 deficiency (PGK1D). PGK1 has 2 isoforms with the molecular mass of 45 kDa and 41 kDa.

Publications

Species	Application	Title
human	WB,IHC	Mol Cancer; Long non-coding RNA GBCDRlnc1 induces chemoresistance of gallbladder cancer cells by activating autophagy.; Authors - Qiang Cai ; KD Validated
human	WB	Cell Death Dis; ACTL6A regulates follicle-stimulating hormone-driven glycolysis in ovarian cancer cells via PGK1.; Authors - Jiawen Zhang
human	IHC	Cell Rep; Enhancer Reprogramming Confers Dependence on Glycolysis and IGF Signaling in KMT2D Mutant Melanoma.; Authors - Mayinuer Maitituoheti
human	IHC	Pregnancy Hypertens; Dysfunction of pseudogene PGK1P2 is involved in preeclampsia by acting as a competing endogenous RNA of PGK1.; Authors - Jing Tong
human	WB,IHC	Am J Transl Res; Collagen prolyl 4-hydroxylase 2 predicts worse prognosis and promotes glycolysis in cervical cancer.; Authors - Qingxian Li
human	WB	J Cancer; Peri-tumoral brain edema associated with glioblastoma correlates with tumor recurrence.; Authors - Xingping Qin