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  • KD/KO Validated

PGK1 Polyclonal antibody

PGK1 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 17811-1-AP

Synonyms

MIG10, OK/SW cl.110, PGK1, PGKA, phosphoglycerate kinase 1, Primer recognition protein 2, PRP 2



Tested Applications

Positive WB detected inHeLa cells, human stomach tissue, PC-3 cells, mouse brain tissue, HEK-293 cells, A431 cells, rat liver tissue, mouse liver tissue, HepG2 cells
Positive IHC detected inhuman hepatocirrhosis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

17811-1-AP targets PGK1 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PGK1 fusion protein Ag12119
Full Name phosphoglycerate kinase 1
Calculated molecular weight 417 aa, 45 kDa
Observed molecular weight 40-45 kDa
GenBank accession numberBC103752
Gene symbol PGK1
Gene ID (NCBI) 5230
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The PGK1 gene encodes phosphoglycerate kinase-1, also known as ATP:3-phosphoglycerate 1-phosphotransferase (EC 2.7.2.3), which catalyzes the reversible conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate during glycolysis, generating one molecule of ATP. It Belongs to the phosphoglycerate kinase family and defects in PGK1 are the cause of phosphoglycerate kinase 1 deficiency (PGK1D). PGK1 has 2 isoforms with the molecular mass of 45 kDa and 41 kDa.

Protocols

Product Specific Protocols
WB protocol for PGK1 antibody 17811-1-APDownload protocol
IHC protocol for PGK1 antibody 17811-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Mol Cancer

Long non-coding RNA GBCDRlnc1 induces chemoresistance of gallbladder cancer cells by activating autophagy.

Authors - Qiang Cai
  • KD Validated
humanWB

Cell Death Dis

ACTL6A regulates follicle-stimulating hormone-driven glycolysis in ovarian cancer cells via PGK1.

Authors - Jiawen Zhang
humanIHC

Cell Rep

Enhancer Reprogramming Confers Dependence on Glycolysis and IGF Signaling in KMT2D Mutant Melanoma.

Authors - Mayinuer Maitituoheti
humanWB,IHC

Am J Transl Res

Collagen prolyl 4-hydroxylase 2 predicts worse prognosis and promotes glycolysis in cervical cancer.

Authors - Qingxian Li
humanWB

J Cancer

Peri-tumoral brain edema associated with glioblastoma correlates with tumor recurrence.

Authors - Xingping Qin

Analyst

An iTRAQ based quantitative proteomic strategy to explore novel secreted proteins in metastatic hepatocellular carcinoma cell lines.

Authors - Yu Yanyan Y