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- KD/KO Validated
PGK1 Polyclonal antibody
PGK1 Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 17811-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | A431 cells, HEK-293 cells, human stomach tissue, mouse brain tissue, HepG2 cells, rat liver tissue, mouse liver tissue, HeLa cells, NIH/3T3 cells, PC-12 cells, PC-3 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:10000 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 5 publications below |
WB | See 37 publications below |
IHC | See 11 publications below |
IF | See 2 publications below |
IP | See 4 publications below |
Product Information
17811-1-AP targets PGK1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | PGK1 fusion protein Ag12119 |
Full Name | phosphoglycerate kinase 1 |
Calculated molecular weight | 417 aa, 45 kDa |
Observed molecular weight | 40-45 kDa |
GenBank accession number | BC103752 |
Gene symbol | PGK1 |
Gene ID (NCBI) | 5230 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The PGK1 gene encodes phosphoglycerate kinase-1, also known as ATP:3-phosphoglycerate 1-phosphotransferase (EC 2.7.2.3), which catalyzes the reversible conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate during glycolysis, generating one molecule of ATP. It Belongs to the phosphoglycerate kinase family and defects in PGK1 are the cause of phosphoglycerate kinase 1 deficiency (PGK1D). PGK1 has 2 isoforms with the molecular mass of 45 kDa and 41 kDa.
Protocols
Product Specific Protocols | |
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WB protocol for PGK1 antibody 17811-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Mol Cancer Long non-coding RNA GBCDRlnc1 induces chemoresistance of gallbladder cancer cells by activating autophagy.
| ||
Cell Death Dis ACTL6A regulates follicle-stimulating hormone-driven glycolysis in ovarian cancer cells via PGK1. | ||
Cell Rep Enhancer Reprogramming Confers Dependence on Glycolysis and IGF Signaling in KMT2D Mutant Melanoma. | ||
Cell Death Dis Reciprocal regulation of LOXL2 and HIF1α drives the Warburg effect to support pancreatic cancer aggressiveness. | ||
Cell Death Dis PGK1 represses autophagy-mediated cell death to promote the proliferation of liver cancer cells by phosphorylating PRAS40.
| ||
Adv Sci (Weinh) Transcriptional Repression of Aerobic Glycolysis by OVOL2 in Breast Cancer. |