PGK1 Polyclonal antibody

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  WB analysis using 17811-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17811-1-AP (PGK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 17811-1-AP

  WB result of PGK1 antibody (17811-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PGK1 transfected HeLa cells.

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  WB analysis of HepG2 using 17811-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17811-1-AP (PGK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 17811-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 17811-1-AP (PGK1 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

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  IP experiment of SW 1990 using 17811-1-AP

  IP result of anti-PGK1 (IP:17811-1-AP, 4ug; Detection:17811-1-AP 1:2000) with SW 1990 cells lysate 2800 ug.

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  IP experiment of HeLa using 17811-1-AP

  IP result of anti-PGK1 (IP:17811-1-AP, 4ug; Detection:17811-1-AP 1:10000) with HeLa cells lysate 980 ug.

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  IF Staining of A431 using 17811-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using PGK1 antibody (17811-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of HepG2 using 17811-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PGK1 antibody (17811-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• Featured Product
• KD/KO Validated

PGK1 Polyclonal Antibody for WB, IP, ELISA

Cat No. 17811-1-AP

Publications(94)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, IP, CoIP, ELISA

Synonyms

MIG10, OK/SW cl.110, PGK1, PGKA, phosphoglycerate kinase 1, Primer recognition protein 2, PRP 2

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Tested Applications

• Positive WB detected in: A431 cells, HepG2 cells, HEK-293 cells, HeLa cells, PC-12 cells, PC-3 cells, NIH/3T3 cells, mouse liver tissue, rat liver tissue
• Positive IP detected in: SW 1990 cells, HeLa cells
• Positive IF/ICC detected in: A431 cells, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 8 publications below
• WB: See 81 publications below
• IHC: See 20 publications below
• IF: See 7 publications below
• IP: See 6 publications below
• CoIP: See 5 publications below

Product Information

17811-1-AP targets PGK1 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PGK1 fusion protein Ag12119
• Full Name: phosphoglycerate kinase 1
• Calculated Molecular Weight: 417 aa, 45 kDa
• Observed Molecular Weight: 40-45 kDa
• GenBank Accession Number: BC103752
• Gene Symbol: PGK1
• Gene ID (NCBI): 5230
• RRID: AB_2161218
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P00558
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The PGK1 gene encodes phosphoglycerate kinase-1, also known as ATP:3-phosphoglycerate 1-phosphotransferase (EC 2.7.2.3), which catalyzes the reversible conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate during glycolysis, generating one molecule of ATP. It Belongs to the phosphoglycerate kinase family and defects in PGK1 are the cause of phosphoglycerate kinase 1 deficiency (PGK1D). PGK1 has 2 isoforms with the molecular mass of 45 kDa and 41 kDa.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,IHC	Cancer Cell; Transcriptional Regulation of the Warburg Effect in Cancer by SIX1.; Authors - Ling Li
mouse	IF	Nat Neurosci; A glycolytic shift in Schwann cells supports injured axons.; Authors - Elisabetta Babetto
human	WB,IP,IF	Mol Cell; The long noncoding RNA glycoLINC assembles a lower glycolytic metabolon to promote glycolysis.; Authors - Youming Zhu
human	WB	Nat Commun; Low chorionic villous succinate accumulation associates with recurrent spontaneous abortion risk.; Authors - Xiao-Hui Wang
human	WB	Nat Commun; Fasting inhibits aerobic glycolysis and proliferation in colorectal cancer via the Fdft1-mediated AKT/mTOR/HIF1α pathway suppression.; Authors - Mei-Lin Weng
human	WB,IHC,IF,CoIP	Exp Hematol Oncol; Downregulation of pro-surfactant protein B contributes to the recurrence of early-stage non-small cell lung cancer by activating PGK1-mediated Akt signaling; Authors - Hao Luo