PI3 Kinase p110 Gamma Polyclonal antibody

• 

  WB analysis using 20662-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20662-1-AP (PI3 Kinase p110 Gamma antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 20662-1-AP

  IP result of anti-PI3 Kinase p110 Gamma (IP:20662-1-AP, 5ug; Detection:20662-1-AP 1:300) with mouse liver tissue lysate 4000ug.

• 

  IHC staining of mouse skeletal muscle using 20662-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 20662-1-AP (PI3 Kinase p110 Gamma antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with .

• 

  IHC staining of mouse skeletal muscle using 20662-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 20662-1-AP (PI3 Kinase p110 Gamma antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with .

PI3 Kinase p110 Gamma Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 20662-1-AP

Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat and More (1)

Applications

WB, IHC, IP, ELISA

Synonyms

PIK3CG, EC:2.7.1.137, EC:2.7.11.1, p110gamma, p120 PI3K

---

Tested Applications

• Positive WB detected in: mouse skeletal muscle tissue, rat skeletal muscle tissue
• Positive IP detected in: mouse liver tissue
• Positive IHC detected in: mouse skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IHC: See 1 publications below

Product Information

20662-1-AP targets PI3 Kinase p110 Gamma in WB, IHC, IP, ELISA applications and shows reactivity with mouse, rat samples.

• Tested Reactivity: mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: phosphoinositide-3-kinase, catalytic, gamma polypeptide
• Calculated Molecular Weight: 126 kDa
• Observed Molecular Weight: 100-130 kDa
• GenBank Accession Number: NM_002649
• Gene Symbol: PIK3CG
• Gene ID (NCBI): 5294
• RRID: AB_2878720
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P48736
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Am J Physiol Endocrinol Metab; Frequent and mild scrotal heat stress in mice epigenetically alters glucose metabolism in the male offspring.; Authors - Xiaoyan Wan
mouse	WB	Am J Pathol; Suppression of Overactive Insulin-Like Growth Factor 1 Attenuates Trauma-Induced Heterotopic Ossification in Mice; Authors - Dong Mao
mouse	IHC	Int J Biol Macromol; Water-insoluble dietary fiber from walnut relieves constipation through Limosilactobacillus reuteri-mediated serotonergic synapse and neuroactive ligand-receptor pathways; Authors - Weixing Yang
human	WB	Biofactors; Knockdown of integrin β1 inhibits proliferation and promotes apoptosis in bladder cancer cells; Authors - Jin-Feng Wang