VPS34 (C terminal) Polyclonal antibody

• Featured Product
• KD/KO Validated

VPS34 (C terminal) Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

13

Cat no : 12452-1-AP

Synonyms

hVps34, PI 3 Kinase Class 3, PI 3-kinase p100, PI3K p100, PI3K type 3, PIK3C3, PtdIns 3 kinase type 3, VPS34

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Validation Data Gallery

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  WB analysis of PC-3 using 12452-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 12452-1-AP (VPS34 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 12452-1-AP

  WB result of VPS34 (C terminal) antibody (12452-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-VPS34 (C terminal) transfected Jurkat cells.

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  IHC staining of human prostate cancer using 12452-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12452-1-AP (VPS34 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 12452-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12452-1-AP (VPS34 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of PC-3 using 12452-1-AP

  Immunofluorescent analysis of PC-3 cells using 12452-1-AP (VPS34 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: PC-3 cells, mouse brain tissue, HeLa cells, human testis tissue, human brain tissue, mouse lung tissue, mouse testis tissue, Jurkat cells, rat brain tissue
• Positive IHC detected in: human prostate cancer tissue, human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF): IF : 1:10-1:100

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 12 publications below
• IF: See 2 publications below

Product Information

12452-1-AP targets VPS34 (C terminal) in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: VPS34 (C terminal) fusion protein Ag3110
• Full Name: phosphoinositide-3-kinase, class 3
• Calculated molecular weight: 887 aa, 100 kDa
• Observed molecular weight: 100 kDa
• GenBank accession number: BC033004
• Gene symbol: PIK3C3
• Gene ID (NCBI): 5289
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

PIK3C3(Phosphatidylinositol 3-kinase catalytic subunit type 3) is also named as VPS34 and belongs to the PI3/PI4-kinase family.It is a catalytic subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate which plays a key role in initiation and maturation of autophagosomes.It can form a dimer(PMID:20339072).

Publications

Species	Application	Title
rat	WB	Am J Transl Res; Protective effects of resveratrol on random-pattern skin flap survival: an experimental study.; Authors - Jinti Lin
rat	WB	Cell Death Dis; FGF21 augments autophagy in random-pattern skin flaps via AMPK signaling pathways and improves tissue survival.; Authors - Kailiang Zhou
mouse	WB,IF	J Thromb Haemost; Vps34 derived phosphatidylinositol 3-monophosphate modulates megakaryocyte maturation and proplatelet production through late endosomes/lysosomes.; Authors - Ivana Bertović
mouse	WB	Exp Cell Res; TGF-β1 inhibits the autophagy of podocytes by activating mTORC1 in IgA nephropathy.; Authors - Xing Mao
rat	WB	Am J Transl Res; Hydroxysafflor yellow A promotes multiterritory perforating flap survival: an experimental study.; Authors - Chenxi Zhang
mouse	WB	Front Pharmacol; Betulinic Acid Enhances the Viability of Random-Pattern Skin Flaps by Activating Autophagy.; Authors - Jiafeng Li