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VPS34 (C terminal) Polyclonal antibody
VPS34 (C terminal) Polyclonal Antibody for IF, IHC, WB,ELISA
Cat no : 12452-1-AP
|Positive WB detected in||PC-3 cells, mouse brain tissue, HeLa cells, human testis tissue, human brain tissue, mouse lung tissue, mouse testis tissue, Jurkat cells, rat brain tissue|
|Positive IHC detected in||human prostate cancer tissue, human heart tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||PC-3 cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:100-1:400|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
12452-1-AP targets VPS34 (C terminal) in WB, IP, IHC, IF, CoIP,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||VPS34 (C terminal) fusion protein Ag3110|
|Full Name||phosphoinositide-3-kinase, class 3|
|Calculated molecular weight||887 aa, 100 kDa|
|Observed molecular weight||100 kDa|
|GenBank accession number||BC033004|
|Gene ID (NCBI)||5289|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
PIK3C3(Phosphatidylinositol 3-kinase catalytic subunit type 3) is also named as VPS34 and belongs to the PI3/PI4-kinase family.It is a catalytic subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate which plays a key role in initiation and maturation of autophagosomes.It can form a dimer(PMID:20339072).
|Product Specific Protocols|
|WB protocol for VPS34 (C terminal) antibody 12452-1-AP||Download protocol|
|IHC protocol for VPS34 (C terminal) antibody 12452-1-AP||Download protocol|
|IF protocol for VPS34 (C terminal) antibody 12452-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Am J Transl Res
Protective effects of resveratrol on random-pattern skin flap survival: an experimental study.
Cell Death Dis
FGF21 augments autophagy in random-pattern skin flaps via AMPK signaling pathways and improves tissue survival.
J Thromb Haemost
Vps34 derived phosphatidylinositol 3-monophosphate modulates megakaryocyte maturation and proplatelet production through late endosomes/lysosomes.
Oxid Med Cell Longev
Liraglutide, a TFEB-Mediated Autophagy Agonist, Promotes the Viability of Random-Pattern Skin Flaps.
Exp Cell Res
TGF-β1 inhibits the autophagy of podocytes by activating mTORC1 in IgA nephropathy.
Am J Transl Res
Hydroxysafflor yellow A promotes multiterritory perforating flap survival: an experimental study.