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VPS34 (C terminal) Polyclonal antibody
VPS34 (C terminal) Polyclonal Antibody for IF, IHC, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF,ELISA
Conjugate
Unconjugated
Cat no : 12452-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | PC-3 cells, mouse brain tissue, HeLa cells, human testis tissue, human brain tissue, mouse lung tissue, mouse testis tissue, Jurkat cells, rat brain tissue |
Positive IHC detected in | human prostate cancer tissue, human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | PC-3 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
Immunofluorescence (IF) | IF : 1:10-1:100 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 16 publications below |
IF | See 2 publications below |
Product Information
12452-1-AP targets VPS34 (C terminal) in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | VPS34 (C terminal) fusion protein Ag3110 |
Full Name | phosphoinositide-3-kinase, class 3 |
Calculated molecular weight | 887 aa, 100 kDa |
Observed molecular weight | 100 kDa |
GenBank accession number | BC033004 |
Gene symbol | PIK3C3 |
Gene ID (NCBI) | 5289 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PIK3C3(Phosphatidylinositol 3-kinase catalytic subunit type 3) is also named as VPS34 and belongs to the PI3/PI4-kinase family.It is a catalytic subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate which plays a key role in initiation and maturation of autophagosomes.It can form a dimer(PMID:20339072).
Protocols
Product Specific Protocols | |
---|---|
WB protocol for VPS34 (C terminal) antibody 12452-1-AP | Download protocol |
IHC protocol for VPS34 (C terminal) antibody 12452-1-AP | Download protocol |
IF protocol for VPS34 (C terminal) antibody 12452-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Am J Transl Res Protective effects of resveratrol on random-pattern skin flap survival: an experimental study. | ||
Cell Death Dis FGF21 augments autophagy in random-pattern skin flaps via AMPK signaling pathways and improves tissue survival. | ||
J Thromb Haemost Vps34 derived phosphatidylinositol 3-monophosphate modulates megakaryocyte maturation and proplatelet production through late endosomes/lysosomes. | ||
Exp Cell Res TGF-β1 inhibits the autophagy of podocytes by activating mTORC1 in IgA nephropathy. | ||
Am J Transl Res Hydroxysafflor yellow A promotes multiterritory perforating flap survival: an experimental study. | ||
Front Pharmacol Betulinic Acid Enhances the Viability of Random-Pattern Skin Flaps by Activating Autophagy. |