Tested Applications
| Positive WB detected in | PC-3 cells, Jurkat cells, mouse brain tissue, mouse lung tissue, mouse testis tissue, rat brain tissue |
| Positive IHC detected in | human prostate cancer tissue, human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | PC-3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:300-1:1200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12452-1-AP targets VPS34 (C terminal) in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3110 Product name: Recombinant human PIK3C3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 600-887 aa of BC033004 Sequence: KIRGIIPETATLFKSALMPAQLFFKTEDGGKYPVIFKHGDDLRQDQLILQIISLMDKLLRKENLDLKLTPYKVLATSTKHGFMQFIQSVPVAEVLDTEGSIQNFFRKYAPSENGPNGISAEVMDTYVKSCAGYCVITYILGVGDRHLDNLLLTKTGKLFHIDFGYILGRDPKPLPPPMKLNKEMVEGMGGTQSEQYQEFRKQCYTAFLHLRRYSNLILNLFSLMVDANIPDIALEPDKTVKKVQDKFRLDLSDEEAVHYMQSLIDESVHALFAAVVEQIHKFAQYWRK Predict reactive species |
| Full Name | phosphoinositide-3-kinase, class 3 |
| Calculated Molecular Weight | 887 aa, 100 kDa |
| Observed Molecular Weight | 100 kDa |
| GenBank Accession Number | BC033004 |
| Gene Symbol | VPS34 |
| Gene ID (NCBI) | 5289 |
| RRID | AB_2299709 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8NEB9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PIK3C3(Phosphatidylinositol 3-kinase catalytic subunit type 3) is also named as VPS34 and belongs to the PI3/PI4-kinase family.It is a catalytic subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate which plays a key role in initiation and maturation of autophagosomes.It can form a dimer(PMID:20339072).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for VPS34 (C terminal) antibody 12452-1-AP | Download protocol |
| IHC protocol for VPS34 (C terminal) antibody 12452-1-AP | Download protocol |
| WB protocol for VPS34 (C terminal) antibody 12452-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-BECN1 (Cat# 12452-1-AP) has accumulated 80 citations published in journals such as Autophagy, Nature Communications, Molecular Cancer, Developmental Cell, Cell Death & Disease, Free Radical Biology and Medicine, International Journal of Biological Sciences, Frontiers in Pharmacology, Journal of Neuroinflammation, Oncogene, and PLoS Biology. The associated research covers autophagy regulation, multiple cancers (pancreatic, lung, colorectal, melanoma, small cell lung), spinal cord injury, skin flap survival, neuroinflammation, pyroptosis, ferroptosis, viral infections, diabetic nephropathy, osteoporosis, and aging-related diseases.
| Species | Application | Title |
|---|---|---|
Mol Cancer Overcoming multi-drug resistance in SCLC: a synergistic approach with venetoclax and hydroxychloroquine targeting the lncRNA LYPLAL1-DT/BCL2/BECN1 pathway | ||
Autophagy Kit-mediated autophagy suppression driven by a viral oncoprotein emerges as a crucial survival mechanism in Merkel cell carcinoma | ||
Autophagy Bunyavirus SFTSV NSs utilizes autophagy to escape the antiviral innate immune response | ||
Autophagy Modification of BECN1 by ISG15 Plays a Crucial Role in Autophagy Regulation by Type I IFN/interferon. | ||
Autophagy SHISA5/SCOTIN restrains spontaneous autophagy induction by blocking contact between the ERES and phagophores. |
Reviews
What customers say
One reviewer (5/5) used this VPS34 antibody on mouse colon tissue for both Western blotting (1:1400) and immunofluorescence (1:350). They reported strong, specific signals with good reproducibility across both applications and expressed high satisfaction with overall performance, recommending it for detecting VPS34 expression in colon tissue.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Balawant (Verified Customer) (08-19-2026) | I have used this VPS34 antibody for both Western blotting and immunofluorescence (IF) staining of colon tissue, and it has worked very well. For Western blotting, I used a 1:1400 dilution, and for immunofluorescence, I used a 1:350 dilution. The antibody provided strong and specific signals with good reproducibility. Overall, I am very satisfied with its performance and would recommend it for detecting VPS34 expression in colon tissue.
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