|Positive WB detected in||HepG2 cells, HeLa cells|
|Positive IF detected in||Hela cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
17894-1-AP targets VPS15 in WB, IP, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||VPS15 fusion protein Ag12221|
|Full Name||phosphoinositide-3-kinase, regulatory subunit 4|
|Calculated molecular weight||1358 aa, 153 kDa|
|Observed molecular weight||150 kDa|
|GenBank accession number||BC110318|
|Gene ID (NCBI)||30849|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
PIK3R4(phosphoinositide 3-kinase regulatory subunit 4) is also named as PI3-kinase p150 subunit and belongs to the Ser/Thr protein kinase family. It is a regulatory subunit of the PI3K complex and may regulate membrane trafficking late in the endocytic pathway. This protein can be probably autophosphorylated(PMID:19369195).
Mutations in Vps15 perturb neuronal migration in mice and are associated with neurodevelopmental disease in humans.
Structural basis for VPS34 kinase activation by Rab1 and Rab5 on membranes.
IL-6 regulates autophagy and chemotherapy resistance by promoting BECN1 phosphorylation.
Autophagic elimination of ribosomes during spermiogenesis provides energy for flagellar motility.
J Hazard Mater
Nano-graphene oxide depresses neurotransmission by blocking retrograde transport of mitochondria.
Targeting AR-Beclin 1 Complex-Modulated Growth Factor Signaling Increases the Antiandrogen-Enzalutamide Sensitivity to Better Suppress the Castration-Resistant Prostate Cancer Growth.