VPS15 Polyclonal antibody

VPS15 Polyclonal Antibody for IF, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, CoIP, ELISA

Conjugate

Unconjugated

Publications

8

Cat no : 17894-1-AP

Synonyms

p150, PI3 kinase p150 subunit, PI3K p150, PI3-kinase regulatory subunit 4, PIK3R4, VPS15

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Validation Data Gallery

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  WB analysis of HepG2 using 17894-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17894-1-AP (VPS15 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 17894-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17894-1-AP (VPS15 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 17894-1-AP

  Immunofluorescent analysis of Hela cells, using PIK3R4 antibody 17894-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: HepG2 cells, HeLa cells
• Positive IF detected in: Hela cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 6 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Product Information

17894-1-AP targets VPS15 in WB, IP, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: VPS15 fusion protein Ag12221
• Full Name: phosphoinositide-3-kinase, regulatory subunit 4
• Calculated Molecular Weight: 1358 aa, 153 kDa
• Observed Molecular Weight: 150 kDa
• GenBank Accession Number: BC110318
• Gene Symbol: PIK3R4
• Gene ID (NCBI): 30849
• RRID: AB_2163630
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PIK3R4(phosphoinositide 3-kinase regulatory subunit 4) is also named as PI3-kinase p150 subunit and belongs to the Ser/Thr protein kinase family. It is a regulatory subunit of the PI3K complex and may regulate membrane trafficking late in the endocytic pathway. This protein can be probably autophosphorylated(PMID:19369195).

Publications

Species	Application	Title
mouse	WB	Nat Neurosci; Mutations in Vps15 perturb neuronal migration in mice and are associated with neurodevelopmental disease in humans.; Authors - Thomas Gstrein
human	WB	Nat Commun; Structural basis for VPS34 kinase activation by Rab1 and Rab5 on membranes.; Authors - Shirley Tremel
	IP,CoIP	Nat Commun; IL-6 regulates autophagy and chemotherapy resistance by promoting BECN1 phosphorylation.; Authors - Fuqing Hu
mouse	WB	Dev Cell; Autophagic elimination of ribosomes during spermiogenesis provides energy for flagellar motility.; Authors - Yuqing Lei
rat	WB	J Hazard Mater; Nano-graphene oxide depresses neurotransmission by blocking retrograde transport of mitochondria.; Authors - Yiyuan Kang
		Cancer Lett; Targeting AR-Beclin 1 Complex-Modulated Growth Factor Signaling Increases the Antiandrogen-Enzalutamide Sensitivity to Better Suppress the Castration-Resistant Prostate Cancer Growth.; Authors - Meng Zhang