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PKLR Polyclonal antibody

PKLR Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 22456-1-AP


PK1, PKL, PKLR, PKR, PKRL, Pyruvate kinase 1, Pyruvate kinase isozymes R/L, pyruvate kinase, liver and RBC, R type/L type pyruvate kinase, Red cell/liver pyruvate kinase, RPK

Tested Applications

Positive WB detected inHepG2 cells, mouse skeletal muscle tissue, multi-cells/tissue, mouse muscle/liver tissue, mouse heart tissue, 293 cell, HepG2.MCF7 cells, HeLa cells, MCF-7 cells, human kidney tissue, human liver tissue, NIH/3T3 cells, HEK-293 cells, Raji cells
Positive IHC detected inhuman lung cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22456-1-AP targets PKLR in WB, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PKLR fusion protein Ag17933
Full Name pyruvate kinase, liver and RBC
Calculated Molecular Weight 574 aa, 62 kDa
Observed Molecular Weight 58-62 kDa
GenBank Accession NumberBC025737
Gene Symbol PKLR
Gene ID (NCBI) 5313
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PKLR(Pyruvate kinase isozymes R/L) is also named as PK1,PKL,which is a glycolytic enzyme that catalyzes the transphosphorylation from phosphoenolpyruvate (PEP) to ADP, yielding pyruvate and ATP. It is the last step of the glycolytic pathway and is essentially irreversible.It belongs to the pyruvate kinase family and There are 4 isozymes of pyruvate kinase in mammals: L, R, M1 and M2. L type is major isozyme in the liver, R is found in red cells, M1 is the main form in muscle, heart and brain, and M2 is found in early fetal tissues.Defects in PKLR are the cause of pyruvate kinase hyperactivity (PKHYP) and pyruvate kinase deficiency of red cells (PKRD).It can form a homotetramer(PMID:11960989).


Product Specific Protocols
WB protocol for PKLR antibody 22456-1-APDownload protocol
IHC protocol for PKLR antibody 22456-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Nat Med

Pyruvate kinase M2 activation may protect against the progression of diabetic glomerular pathology and mitochondrial dysfunction.

Authors - Weier Qi

Cell Metab

Multi-Tissue Acceleration of the Mitochondrial Phosphoenolpyruvate Cycle Improves Whole-Body Metabolic Health.

Authors - Abudukadier Abulizi

Mol Ther

Extracellular vesicle-mediated communication between hepatocytes and natural killer cells promotes hepatocellular tumorigenesis.

Authors - Zhijun Liu

PLoS Genet

PMID: 24831725

Authors - Yukimasa Takeda

Int J Mol Sci

Loss of miR-23b/27b/24-1 Cluster Impairs Glucose Tolerance via Glycolysis Pathway in Mice.

Authors - Yong-Hui Jiang

Sci Rep

Up-regulation of PKM2 promote malignancy and related to adverse prognostic risk factor in human gallbladder cancer.

Authors - Wei Lu