Featured Product
KD/KO Validated

PKM1-specific Antibody

Name: PKM1-specific Rabbit Polyclonal antibody
Brand: Proteintech
SKU: 15821-1-AP

PKM1-specific Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

IHC, IP, WB, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 15821-1-AP

Synonyms

CTHBP, OIP 3, OIP3, Opa interacting protein 3, p58, PK2, PK3, PKM, PKM1, PK-M1, PKM1 pyruvate kinase, muscle, Pyruvate kinase 2/3, Pyruvate kinase isozymes M1/M2, Pyruvate kinase muscle isozyme, TCB, THBP1, Tumor M2 PK

Validation Data Gallery

WB analysis of mouse colon tissue using 15821-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15821-1-AP (PKM1-specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours
WB analysis of mouse skeletal muscle tissue using 15821-1-AP
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15821-1-AP (PKM1-specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours
IP experiment of mouse brain tissue using 15821-1-AP
IP Result of anti-PKM1-specific (IP:15821-1-AP, 3ug; Detection:15821-1-AP 1:1000) with mouse brain tissue lysate 4000ug.
IHC staining of human gliomas tissue using 15821-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 15821-1-AP( PKM1-specific antibody at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).
IHC staining of human gliomas tissue using 15821-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 15821-1-AP( PKM1-specific antibody at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

-/ -

Cat No. 15821-1-AP

Freight/Packing: -

Country/Region

Size/Concentration

Quantity

Please visit your regions distributor:

Scandinavian customers can now order directly from Proteintech. Alternatively, you can also order through our approvied distributors Nordic Biosite.

Proteintech Guarantee

Request bulk or custom quote

Chat with a team member

Tested Applications

Positive WB detected in	mouse skeletal muscle tissue, mouse muscle tissues, mouse heart tissue, mouse colon tissue, mouse brain tissue
Positive IP detected in	mouse brain tissue
Positive IHC detected in	human gliomas tissue, human brain tissue, human skeletal muscle tissue, human heart tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)	IHC : 1:100-1:400
Sample-dependent, check data in validation data gallery

Published Applications

KD/KO	See 3 publications below
WB	See 27 publications below
IHC	See 10 publications below
IF	See 2 publications below
IP	See 1 publications below

Product Information

15821-1-AP targets PKM1-specific in IHC, IP, WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Reactivity	human, mouse, rat
Cited Species	human, monkey, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide
Full Name	PKM pyruvate kinase, muscle
Calculated molecular weight	58 kDa
Observed molecular weight	58 kDa
GenBank accession number	NM_182470
Gene symbol	PKM
Gene ID (NCBI)	5315
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20^oC. Aliquoting is unnecessary for -20^oC storage.

Background Information

PKM, also named as OIP3, PK2, PK3, PKM, p58, THBP1, CTHBP and Tumor M2-PK, belongs to the pyruvate kinase family. It is glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. It stimulates POU5F1-mediated transcriptional activation. PKM plays a general role in caspase independent cell death of tumor cells. PKM has 2 isoforms named PKM1 and PKM2. By the upstream metabolite fructose-1,6-bisphosphate, PKM1 is a constitutively active enzyme. The primary pyruvate kinase isoform before tumour development is PK-M1; however, the primary isoform from four independent tumours is PK-M2 (PMID:18337823). This antibody is specific to PKM1 isoform.

Protocols

Product Specific Protocols
WB protocol for PKM1-specific antibody 15821-1-AP	Download protocol
IHC protocol for PKM1-specific antibody 15821-1-AP	Download protocol
IP protocol for PKM1-specific antibody 15821-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
rat	WB	Front Cardiovasc Med Biventricular Increases in Mitochondrial Fission Mediator (MiD51) and Proglycolytic Pyruvate Kinase (PKM2) Isoform in Experimental Group 2 Pulmonary Hypertension-Novel Mitochondrial Abnormalities. Authors - Ping Yu Xiong View Article
human	IHC	Pathol Oncol Res Overexpression of Pyruvate Kinase M2 in Tumor Tissues Is Associated with Poor Prognosis in Patients with Hepatocellular Carcinoma. Authors - Rui Zhao View Article
	WB	Regen Ther Pyruvate kinase M2, but not M1, allele maintains immature metabolic states of murine embryonic stem cells. Authors - Masamitsu Konno View Article
human	IHC	Semin Oncol Reverse Warburg effect in a patient with aggressive B-cell lymphoma: is lactic acidosis a paraneoplastic syndrome? Authors - Ubaldo E Martinez-Outschoorn View Article
human	WB	Biochim Biophys Acta Cancer associated fibroblasts transfer lipids and proteins to cancer cells through cargo vesicles supporting tumor growth. Authors - Alice Santi View Article
human	IHC	Anticancer Res The Lack of Evidence for Correlation of Pyruvate Kinase M2 Expression with Tumor Grade in Non-small Cell Lung Cancer. Authors - Christopher Kobierzycki View Article