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- KD/KO Validated
PKM1-specific Polyclonal antibody
PKM1-specific Polyclonal Antibody for IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IP, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 15821-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | mouse skeletal muscle tissue, mouse muscle tissues, mouse heart tissue, mouse colon tissue, mouse brain tissue |
Positive IP detected in | mouse brain tissue |
Positive IHC detected in | human gliomas tissue, human brain tissue, human skeletal muscle tissue, human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB |
Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 3 publications below |
WB | See 28 publications below |
IHC | See 10 publications below |
IF | See 2 publications below |
IP | See 1 publications below |
Product Information
15821-1-AP targets PKM1-specific in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, monkey, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Peptide |
Full Name | PKM pyruvate kinase, muscle |
Calculated molecular weight | 58 kDa |
Observed molecular weight | 58 kDa |
GenBank accession number | NM_182470 |
Gene symbol | PKM |
Gene ID (NCBI) | 5315 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PKM, also named as OIP3, PK2, PK3, PKM, p58, THBP1, CTHBP and Tumor M2-PK, belongs to the pyruvate kinase family. It is glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. It stimulates POU5F1-mediated transcriptional activation. PKM plays a general role in caspase independent cell death of tumor cells. PKM has 2 isoforms named PKM1 and PKM2. The primary pyruvate kinase isoform before tumour development is PK-M1; however, the primary isoform from four independent tumours is PK-M2 (PMID:18337823). This antibody is specific to PKM1 isoform.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for PKM1-specific antibody 15821-1-AP | Download protocol |
IHC protocol for PKM1-specific antibody 15821-1-AP | Download protocol |
IP protocol for PKM1-specific antibody 15821-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Front Cardiovasc Med Biventricular Increases in Mitochondrial Fission Mediator (MiD51) and Proglycolytic Pyruvate Kinase (PKM2) Isoform in Experimental Group 2 Pulmonary Hypertension-Novel Mitochondrial Abnormalities. | ||
Pathol Oncol Res Overexpression of Pyruvate Kinase M2 in Tumor Tissues Is Associated with Poor Prognosis in Patients with Hepatocellular Carcinoma. | ||
Regen Ther Pyruvate kinase M2, but not M1, allele maintains immature metabolic states of murine embryonic stem cells. | ||
Semin Oncol Reverse Warburg effect in a patient with aggressive B-cell lymphoma: is lactic acidosis a paraneoplastic syndrome? | ||
Biochim Biophys Acta Cancer associated fibroblasts transfer lipids and proteins to cancer cells through cargo vesicles supporting tumor growth. | ||
Anticancer Res The Lack of Evidence for Correlation of Pyruvate Kinase M2 Expression with Tumor Grade in Non-small Cell Lung Cancer. |