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PKM1-specific Antibody 32 Publications

Rabbit Polyclonal| Catalog number: 15821-1-AP

Featured Product KD/KO validated

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Con: 68 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, ELISA
Application not tested? Please see our Guarantee


Images See all

PKM1-specific Antibody WB HEK-293 cells 15821-1-AP

WB result of PKM1 antibody (15821-1-AP, 1:1000) with si-Control and si-PKM1 transfected HEK293 cells.

PKM1-specific Antibody WB mouse skeletal muscle tissue 15821-1-AP

mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15821-1-AP (PKM1-specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

PKM1-specific Antibody IP mouse brain tissue 15821-1-AP

IP Result of anti-PKM1-specific (IP:15821-1-AP, 3ug; Detection:15821-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

PKM1-specific Antibody IHC human gliomas tissue 15821-1-AP

Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 15821-1-AP( PKM1-specific antibody at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

PKM1-specific Antibody IHC human gliomas tissue 15821-1-AP

Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 15821-1-AP( PKM1-specific antibody at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

PKM1-specific Antibody IF HeLa cells 15821-1-AP

Immunofluorescent analysis of HeLa cells using 15821-1-AP(PKM1-specific antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG

Species specificity:
human, mouse, rat, monkey
Positive WB detected in:
mouse skeletal muscle tissue, multi-cells/tissue, mouse muscle/liver tissues, mouse heart tissue, 293 cell, HepG2/MCF7 cells, mouse brain tissue, HEK-293 cells
Positive IP detected in:
mouse brain tissue
Positive IHC detected in:
human gliomas tissue, human skeletal muscle tissue, human brain tissue, human heart tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HeLa cells
Recommended dilution:
WB : 1:1000-1:4000
IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
IHC : 1:100-1:400
IF : 1:20-1:200
Sample-dependent, check data in Validation tab

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Peptide
Full name:
PKM2 pyruvate kinase, muscle
Calculated molecular weight:
58 kDa
Observed molecular weight:
58 kDa
GenBank accession number:
NM_182470
Gene ID (NCBI):
5315
Gene symbol
PKM2
Synonyms
CTHBP, OIP 3, OIP3, Opa interacting protein 3, p58, PK2, PK3, PKM, PKM1, PK-M1, PKM1 pyruvate kinase, muscle, Pyruvate kinase 2/3, Pyruvate kinase isozymes M1/M2, Pyruvate kinase muscle isozyme, TCB, THBP1, Tumor M2 PK

Background

PKM, also named as OIP3, PK2, PK3, PKM, p58, THBP1, CTHBP and Tumor M2-PK, belongs to the pyruvate kinase family. It is glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. It stimulates POU5F1-mediated transcriptional activation. PKM plays a general role in caspase independent cell death of tumor cells. PKM has 2 isoforms named PKM1 and PKM2. By the upstream metabolite fructose-1,6-bisphosphate, PKM1 is a constitutively active enzyme.The primary pyruvate kinase isoform before tumour development is PK-M1; however, the primary isoform from four independent tumours is PK-M2 (PMID:18337823). This antibody is specific to PKM1 isoform.


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