- Featured Product
- KD/KO Validated
PKM1-specific Polyclonal Antibody for IHC, IP, WB, ELISA
Cat no : 15821-1-AP
CTHBP, OIP 3, OIP3, Opa interacting protein 3, p58, PK2, PK3, PKM, PKM1, PK-M1, PKM1 pyruvate kinase, muscle, Pyruvate kinase 2/3, Pyruvate kinase isozymes M1/M2, Pyruvate kinase muscle isozyme, TCB, THBP1, Tumor M2 PK
|Positive WB detected in||mouse skeletal muscle tissue, mouse muscle tissues, mouse heart tissue, mouse colon tissue, mouse brain tissue|
|Positive IP detected in||mouse brain tissue|
|Positive IHC detected in||human gliomas tissue, human brain tissue, human skeletal muscle tissue, human heart tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:100-1:400|
|Sample-dependent, check data in validation data gallery|
15821-1-AP targets PKM1-specific in IHC, IP, WB, ELISA applications and shows reactivity with human, mouse, rat samples.
|Reactivity||human, mouse, rat|
|Cited Species||human, monkey, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Full Name||PKM pyruvate kinase, muscle|
|Calculated molecular weight||58 kDa|
|Observed molecular weight||58 kDa|
|GenBank accession number||NM_182470|
|Gene ID (NCBI)||5315|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20oC. Aliquoting is unnecessary for -20oC storage.|
PKM, also named as OIP3, PK2, PK3, PKM, p58, THBP1, CTHBP and Tumor M2-PK, belongs to the pyruvate kinase family. It is glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. It stimulates POU5F1-mediated transcriptional activation. PKM plays a general role in caspase independent cell death of tumor cells. PKM has 2 isoforms named PKM1 and PKM2. By the upstream metabolite fructose-1,6-bisphosphate, PKM1 is a constitutively active enzyme. The primary pyruvate kinase isoform before tumour development is PK-M1; however, the primary isoform from four independent tumours is PK-M2 (PMID:18337823). This antibody is specific to PKM1 isoform.
Front Cardiovasc Med
Biventricular Increases in Mitochondrial Fission Mediator (MiD51) and Proglycolytic Pyruvate Kinase (PKM2) Isoform in Experimental Group 2 Pulmonary Hypertension-Novel Mitochondrial Abnormalities.
Pathol Oncol Res
Overexpression of Pyruvate Kinase M2 in Tumor Tissues Is Associated with Poor Prognosis in Patients with Hepatocellular Carcinoma.
Pyruvate kinase M2, but not M1, allele maintains immature metabolic states of murine embryonic stem cells.
Reverse Warburg effect in a patient with aggressive B-cell lymphoma: is lactic acidosis a paraneoplastic syndrome?
Biochim Biophys Acta
Cancer associated fibroblasts transfer lipids and proteins to cancer cells through cargo vesicles supporting tumor growth.
The Lack of Evidence for Correlation of Pyruvate Kinase M2 Expression with Tumor Grade in Non-small Cell Lung Cancer.