PKM1-specific Polyclonal antibody

• Featured Product
• KD/KO Validated

PKM1-specific Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IP, IHC, IF

Conjugate

Unconjugated

Publications

48

Cat no : 15821-1-AP

Synonyms

CTHBP, OIP 3, OIP3, Opa interacting protein 3, p58, PK2, PK3, PKM, PKM1, PK-M1, PKM1 pyruvate kinase, muscle, PKM1-specific, Pyruvate kinase 2/3, Pyruvate kinase isozymes M1/M2, Pyruvate kinase muscle isozyme, TCB, THBP1, Tumor M2 PK

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Validation Data Gallery

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  WB analysis using 15821-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15821-1-AP (PKM1-specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 15821-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15821-1-AP (PKM1-specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 15821-1-AP

  IP Result of anti-PKM1-specific (IP:15821-1-AP, 3ug; Detection:15821-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

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  IHC staining of human gliomas using 15821-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 15821-1-AP (PKM1-specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 15821-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 15821-1-AP (PKM1-specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse skeletal muscle tissue, 293 cell, HepG2/MCF7 cells, mouse brain tissue, mouse colon tissue, mouse heart tissue, mouse muscle/liver tissues, multi-cells/tissue, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human gliomas tissue, human brain tissue, human heart tissue, human skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
• Immunohistochemistry (IHC): IHC : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 3 publications below
• WB: See 40 publications below
• IHC: See 11 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Product Information

15821-1-AP targets PKM1-specific in WB, IP, IHC, IF applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, monkey, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: PKM pyruvate kinase, muscle
• Calculated molecular weight: 58 kDa
• Observed molecular weight: 58 kDa
• GenBank accession number: NM_182470
• Gene symbol: PKM
• Gene ID (NCBI): 5315
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

PKM, also named as OIP3, PK2, PK3, PKM, p58, THBP1, CTHBP and Tumor M2-PK, belongs to the pyruvate kinase family. It is glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. It stimulates POU5F1-mediated transcriptional activation. PKM plays a general role in caspase independent cell death of tumor cells. PKM has 2 isoforms named PKM1 and PKM2. The primary pyruvate kinase isoform before tumour development is PK-M1; however, the primary isoform from four independent tumours is PK-M2 (PMID:18337823). This antibody is specific to PKM1 isoform.

Publications

Species	Application	Title
rat	WB	Front Cardiovasc Med; Biventricular Increases in Mitochondrial Fission Mediator (MiD51) and Proglycolytic Pyruvate Kinase (PKM2) Isoform in Experimental Group 2 Pulmonary Hypertension-Novel Mitochondrial Abnormalities.; Authors - Ping Yu Xiong
human	IHC	Pathol Oncol Res; Overexpression of Pyruvate Kinase M2 in Tumor Tissues Is Associated with Poor Prognosis in Patients with Hepatocellular Carcinoma.; Authors - Rui Zhao
	WB	Regen Ther; Pyruvate kinase M2, but not M1, allele maintains immature metabolic states of murine embryonic stem cells.; Authors - Masamitsu Konno
human	IHC	Semin Oncol; Reverse Warburg effect in a patient with aggressive B-cell lymphoma: is lactic acidosis a paraneoplastic syndrome?; Authors - Ubaldo E Martinez-Outschoorn
human	WB	Biochim Biophys Acta; Cancer associated fibroblasts transfer lipids and proteins to cancer cells through cargo vesicles supporting tumor growth.; Authors - Alice Santi
human	IHC,WB	Aging (Albany NY); Transcriptional dysregulation of TRIM29 promotes colorectal cancer carcinogenesis via pyruvate kinase-mediated glucose metabolism.; Authors - Jing Han

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