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PKM2-specific Polyclonal antibody
PKM2-specific Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Cat no : 15822-1-AP
Validation Data Gallery
|Positive WB detected in||A549 cells, HeLa cells, HEK-293 cells, MCF-7 cells, Recombinant protein, mouse brain tissue, N/A, HEK-293T cells, mouse stomach tissue|
|Positive IP detected in||A549 cells, MCF-7 cells|
|Positive IHC detected in||human lung cancer tissue, human colon cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||MCF-7 cells|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:1000-1:8000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:100-1:400|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
15822-1-AP targets PKM2-specific in WB, IP, IHC, IF, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse, rat, chicken|
|Host / Isotype||Rabbit / IgG|
|Full Name||PKM pyruvate kinase, muscle|
|Calculated molecular weight||58 kDa|
|Observed molecular weight||58 kDa|
|GenBank accession number||NM_002654|
|Gene ID (NCBI)||5315|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
PKM, also named as OIP3, PK2, PK3, PKM, p58, THBP1, CTHBP and Tumor M2-PK, belongs to the pyruvate kinase family. It is glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. It stimulates POU5F1-mediated transcriptional activation. PKM plays a general role in caspase independent cell death of tumor cells. PKM has 2 isoforms named as PKM1/M2. The activity of the M2 isoform (but not the M1 isoform) can be inhibited by tyrosine kinase signalling in tumourcells. The primary pyruvate kinase isoform before tumour development is PK-M1; however, the primary isoform from four independent tumours is PK-M2 (PMID:18337823). The immunogen of this antibody is M2 isoform and this antibody is specific to PKM2 isoform.
|Product Specific Protocols|
|WB protocol for PKM2-specific antibody 15822-1-AP||Download protocol|
|IHC protocol for PKM2-specific antibody 15822-1-AP||Download protocol|
|IF protocol for PKM2-specific antibody 15822-1-AP||Download protocol|
|IP protocol for PKM2-specific antibody 15822-1-AP||Download protocol|
|Click here to view our Standard Protocols|
The M2 splice isoform of pyruvate kinase is important for cancer metabolism and tumour growth.
The long noncoding RNA glycoLINC assembles a lower glycolytic metabolon to promote glycolysis.
A Peptide Encoded by a Putative lncRNA HOXB-AS3 Suppresses Colon Cancer Growth.
PKM2 allosteric converter: A self-assembly peptide for suppressing renal cell carcinoma and sensitizing chemotherapy
Interactome analysis reveals that lncRNA HULC promotes aerobic glycolysis through LDHA and PKM2.
Proc Natl Acad Sci U S A
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Shinford (Verified Customer) (09-24-2023)
The antibody concentration of 1:1000 worked well, and bands could be detected in a variety of cell lines. There were slight non-specific bands in some tissue and organ cells.
Kristian (Verified Customer) (06-07-2021)
PKM2 (red) is detected in proximale tubules where HIF1A-signaling is activated.
Kishor (Verified Customer) (02-22-2019)
I am very much satisfied with almost all antibodies from Proteintech. This antibody is working very good at 1:1000 dilution for human cancer and primary cells.