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  • KD/KO Validated

PKM2-specific Polyclonal antibody

PKM2-specific Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat and More (2)





Cat no : 15822-1-AP


CTHBP, OIP 3, OIP3, Opa interacting protein 3, p58, PK2, PK3, PKM, PKM2, PK-M2, PKM2 pyruvate kinase, muscle, pmk 2 specific, Pyruvate kinase 2/3, Pyruvate kinase isozymes M1/M2, Pyruvate kinase muscle isozyme, TCB, THBP1, Tumor M2 PK

Tested Applications

Positive WB detected inA549 cells, 293 cell, C6 cells, HEK-293 cells, HeLa cells, mouse brain tissue, N/A, Recombinant protein, HEK-293T cells, MCF-7 cells, mouse stomach tissue
Positive IP detected inA549 cells, MCF-7 cells
Positive IHC detected inhuman lung cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells, MCF-7 cells

Recommended dilution

Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15822-1-AP targets PKM2-specific in WB, IP, IF, IHC, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, chicken, bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name PKM pyruvate kinase, muscle
Calculated Molecular Weight 58 kDa
Observed Molecular Weight 58 kDa
GenBank Accession NumberNM_002654
Gene Symbol PKM
Gene ID (NCBI) 5315
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PKM, also named as OIP3, PK2, PK3, PKM, p58, THBP1, CTHBP and Tumor M2-PK, belongs to the pyruvate kinase family. It is glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. It stimulates POU5F1-mediated transcriptional activation. PKM plays a general role in caspase independent cell death of tumor cells. PKM has 2 isoforms named as PKM1/M2. The activity of the M2 isoform (but not the M1 isoform) can be inhibited by tyrosine kinase signalling in tumourcells. The primary pyruvate kinase isoform before tumour development is PK-M1; however, the primary isoform from four independent tumours is PK-M2 (PMID:18337823). The immunogen of this antibody is M2 isoform and this antibody is specific to PKM2 isoform.


Product Specific Protocols
WB protocol for PKM2-specific antibody 15822-1-APDownload protocol
IHC protocol for PKM2-specific antibody 15822-1-APDownload protocol
IF protocol for PKM2-specific antibody 15822-1-APDownload protocol
IP protocol for PKM2-specific antibody 15822-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols




The M2 splice isoform of pyruvate kinase is important for cancer metabolism and tumour growth.

Authors - Christofk Heather R HR
  • KD Validated

Mol Cell

The long noncoding RNA glycoLINC assembles a lower glycolytic metabolon to promote glycolysis.

Authors - Youming Zhu

Mol Cell

A Peptide Encoded by a Putative lncRNA HOXB-AS3 Suppresses Colon Cancer Growth.

Authors - Jin-Zhou Huang


PKM2 allosteric converter: A self-assembly peptide for suppressing renal cell carcinoma and sensitizing chemotherapy

Authors - Lu Wang

Nat Commun

Interactome analysis reveals that lncRNA HULC promotes aerobic glycolysis through LDHA and PKM2.

Authors - Chunqing Wang

Clin Transl Med

USP7 promotes non-small-cell lung cancer cell glycolysis and survival by stabilizing and activating c-Abl

Authors - Yuanming He


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Shinford (Verified Customer) (09-24-2023)

The antibody concentration of 1:1000 worked well, and bands could be detected in a variety of cell lines. There were slight non-specific bands in some tissue and organ cells.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Tumor cell lines derived from multiple systems, tissues and organs。
PKM2-specific Antibody Western Blot validation (1:1000 dilution) in Tumor cell lines derived from multiple systems, tissues and organs。 (Cat no:15822-1-AP)

Kristian (Verified Customer) (06-07-2021)

PKM2 (red) is detected in proximale tubules where HIF1A-signaling is activated.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: Transgenic mouse kidney (TRACK)
PKM2-specific Antibody Immunofluorescence validation (1:200 dilution) in Transgenic mouse kidney (TRACK) (Cat no:15822-1-AP)

Kishor (Verified Customer) (02-22-2019)

I am very much satisfied with almost all antibodies from Proteintech. This antibody is working very good at 1:1000 dilution for human cancer and primary cells.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: H69, HUCCT-1
PKM2-specific Antibody Western Blot, validation (1:1000 dilution) in H69, HUCCT-1 (Cat no:15822-1-AP)