|Positive WB detected in
|mouse brain tissue
|Positive IHC detected in
|human kidney tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive FC detected in
|Western Blot (WB)
|WB : 1:200-1:1000
|IHC : 1:50-1:500
|Flow Cytometry (FC)
|FC : 0.20 ug per 10^6 cells in a 100 µl suspension
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
The immunogen of 23795-1-AP is PLXNB1 Fusion Protein expressed in E. coli.
|human, mouse, rat
|Host / Isotype
|Rabbit / IgG
|PLXNB1 fusion protein Ag20719
|Calculated molecular weight
|2135 aa, 232 kDa
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen Affinity purified
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Semaphorins, originally identified as axon guidance molecules, have also been implicated in angiogenesis, immunoregulation, and cancer (PMID: 16584533). Plexins are main receptors for semaphorins and are thought to control many of the functional effects of semaphorins. PLXNB1 is one of the three plexin-B family members. It is a high-affinity receptor for semaphorin 4D (SEMA4D). Interaction of SEMA4D and PLXNB1 has important physiologic effects in the immune and nervous systems, and is also involved in tumor progression, especially in tumor angiogenesis (PMID: 20858260).
Cancer Immunol Res
Semaphorin4D Inhibition Improves Response to Immune-Checkpoint Blockade via Attenuation of MDSC Recruitment and Function.
J Transl Med
SEMA4D/PlexinB1 promotes AML progression via activation of PI3K/Akt signaling.
Ann Transl Med
Proteomics and metabolomics of raw rhubarb and wine-processed rhubarb in the treatment of rats with intracerebral hemorrhage.
PLXNB1 mutations in the etiology of idiopathic hypogonadotropic hypogonadism.
Ann Transl Med
Methylation-based epigenetic studies and gene integration analysis of preeclampsia
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Boyan (Verified Customer) (10-04-2021)
calculated size 230 kd. this antibody recognized major bands at 35, 30 kd.