PLXNB1 Polyclonal antibody

PLXNB1 Polyclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 23795-1-AP

Synonyms

KIAA0407, plexin B1, PLXN5, PLXNB1, Semaphorin receptor SEP, SEP



Tested Applications

Positive WB detected inmouse brain tissue
Positive IHC detected inhuman kidney tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 23795-1-AP is PLXNB1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PLXNB1 fusion protein Ag20719
Full Name plexin B1
Calculated molecular weight 2135 aa, 232 kDa
Observed molecular weight 210 kDa
GenBank accession numberBC146793
Gene symbol PLXNB1
Gene ID (NCBI) 5364
RRIDAB_2879326
Conjugate Unconjugated
Form Liquid
Purification Method Antigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Semaphorins, originally identified as axon guidance molecules, have also been implicated in angiogenesis, immunoregulation, and cancer (PMID: 16584533). Plexins are main receptors for semaphorins and are thought to control many of the functional effects of semaphorins. PLXNB1 is one of the three plexin-B family members. It is a high-affinity receptor for semaphorin 4D (SEMA4D). Interaction of SEMA4D and PLXNB1 has important physiologic effects in the immune and nervous systems, and is also involved in tumor progression, especially in tumor angiogenesis (PMID: 20858260).

Protocols

Product Specific Protocols
WB protocol for PLXNB1 antibody 23795-1-APDownload protocol
IHC protocol for PLXNB1 antibody 23795-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseFC

Cancer Immunol Res

Semaphorin4D Inhibition Improves Response to Immune-Checkpoint Blockade via Attenuation of MDSC Recruitment and Function.

Authors - Paul E Clavijo
humanWB

J Transl Med

SEMA4D/PlexinB1 promotes AML progression via activation of PI3K/Akt signaling.

Authors - Lu Liu
ratWB

Ann Transl Med

Proteomics and metabolomics of raw rhubarb and wine-processed rhubarb in the treatment of rats with intracerebral hemorrhage.

Authors - Kun Wang
mouseIF

J Neuroendocrinol

PLXNB1 mutations in the etiology of idiopathic hypogonadotropic hypogonadism.

Authors - Bradley A Welch
humanIHC

Ann Transl Med

Methylation-based epigenetic studies and gene integration analysis of preeclampsia

Authors - Lei Jiang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Boyan (Verified Customer) (10-04-2021)

calculated size 230 kd. this antibody recognized major bands at 35, 30 kd.

  • Applications: Western Blot