|Positive WB detected in||HEK-293 cells, HeLa cells|
|Positive IP detected in||HEK-293 cells|
|Positive IHC detected in||human liver tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:200-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:100-1:400|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
The immunogen of 21041-1-AP is PML Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||PML fusion protein Ag15377|
|Full Name||promyelocytic leukemia|
|Calculated molecular weight||882 aa, 98 kDa|
|Observed molecular weight||120 kDa|
|GenBank accession number||BC020994|
|Gene ID (NCBI)||5371|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
PML, also named as MYL, RNF71 and TRIM19, is a member of RBCC/TRIM family of proteins that possess a RING finger domain, B-box, and coiled-coil domain. It functions as tumor suppressor. PML is required for normal, caspase-dependent apoptosis in response to DNA damage, FAS, TNF, or interferons. It plays a role in transcription regulation, DNA damage response, DNA repair and chromatin organization. PML plays a role in processes regulated by retinoic acid, regulation of cell division, terminal differentiation of myeloid precursor cells and differentiation of neural progenitor cells. It regulates PTEN compartmentalization through the inhibition of USP7-mediated deubiquitinylation. A large number of alternative spliced transcripts are synthesized from the PML gene, resulting in a variety of PML proteins ranging in molecular weight from 48-97 kDa (PMID:11704850). And it can be detected as 70-130 kDa or larger due to the modification (especially SUMO) (PMID: 16778193, 22438555).
|Product Specific Protocols|
|WB protocol for PML antibody 21041-1-AP||Download protocol|
|IHC protocol for PML antibody 21041-1-AP||Download protocol|
|IF protocol for PML antibody 21041-1-AP||Download protocol|
|IP protocol for PML antibody 21041-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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Autophagic degradation of PML promotes susceptibility to HSV-1 by stress-induced corticosterone.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Eishi (Verified Customer) (11-17-2017)
Dear colleagues,find attached the WB results. Experimental conditions:Whole protein extraction from mouse embryonic stem cells 25ug protein/laneTransfer 1h30min, 100V, 4C1AB incubation: o/n 4C, 1:500 dilution in milk2AB incubation: 1h RT, 1:20000 dilution in milkDevelopment with femtoThe observed MW is much higher than the expected, with a strong band around 200KDa. This raises the question if the antibody binds specifically to our protein of interest