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  • KD/KO Validated

PML Polyclonal antibody

PML Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 21041-1-AP

Synonyms

MYL, PML, PP8675, promyelocytic leukemia, RING finger protein 71, RNF71, TRIM19



Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 21041-1-AP is PML Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PML fusion protein Ag15377
Full Name promyelocytic leukemia
Calculated molecular weight 882 aa, 98 kDa
Observed molecular weight 120 kDa
GenBank accession numberBC020994
Gene symbol PML
Gene ID (NCBI) 5371
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PML, also named as MYL, RNF71 and TRIM19, is a member of RBCC/TRIM family of proteins that possess a RING finger domain, B-box, and coiled-coil domain. It functions as tumor suppressor. PML is required for normal, caspase-dependent apoptosis in response to DNA damage, FAS, TNF, or interferons. It plays a role in transcription regulation, DNA damage response, DNA repair and chromatin organization. PML plays a role in processes regulated by retinoic acid, regulation of cell division, terminal differentiation of myeloid precursor cells and differentiation of neural progenitor cells. It regulates PTEN compartmentalization through the inhibition of USP7-mediated deubiquitinylation. A large number of alternative spliced transcripts are synthesized from the PML gene, resulting in a variety of PML proteins ranging in molecular weight from 48-97 kDa (PMID:11704850). And it can be detected as 70-130 kDa or larger due to the modification (especially SUMO) (PMID: 16778193, 22438555).

Protocols

Product Specific Protocols
WB protocol for PML antibody 21041-1-APDownload protocol
IHC protocol for PML antibody 21041-1-APDownload protocol
IF protocol for PML antibody 21041-1-APDownload protocol
IP protocol for PML antibody 21041-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Rep

SFTSV Infection Induces BAK/BAX-Dependent Mitochondrial DNA Release to Trigger NLRP3 Inflammasome Activation.

Authors - Shufen Li
humanWB

Front Oncol

Identification of a Two-Gene (PML-EPB41) Signature With Independent Prognostic Value in Osteosarcoma.

Authors - Shengye Liu
humanWB

Molecules

A Comparative Quantitative Proteomic Analysis of HCMV-Infected Cells Highlights pUL138 as a Multifunctional Protein.

Authors - Yang Li
humanWB

Cell Commun Signal

PML-II regulates ERK and AKT signal activation and IFNα-induced cell death.

Authors - Xueqiong Meng
  • KD Validated
humanWB,IF

J Ginseng Res

20(S)-ginsenoside Rh2 induces caspase-dependent promyelocytic leukemia-retinoic acid receptor A degradation in NB4 cells via Akt/Bax/caspase9 and TNF-α/caspase8 signaling cascades.

Authors - Sirui Zhu
humanIHC,IF

Theranostics

Autophagic degradation of PML promotes susceptibility to HSV-1 by stress-induced corticosterone.

Authors - Wen Li

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Eishi (Verified Customer) (11-17-2017)

Dear colleagues,find attached the WB results. Experimental conditions:Whole protein extraction from mouse embryonic stem cells 25ug protein/laneTransfer 1h30min, 100V, 4C1AB incubation: o/n 4C, 1:500 dilution in milk2AB incubation: 1h RT, 1:20000 dilution in milkDevelopment with femtoThe observed MW is much higher than the expected, with a strong band around 200KDa. This raises the question if the antibody binds specifically to our protein of interest

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Protein
PML Antibody Western Blot validation (1:500 dilution) in Protein (Cat no:21041-1-AP)