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PMPCB Polyclonal antibody

PMPCB Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 16064-1-AP

Synonyms

Beta MPP, MPP11, MPPB, MPPP52, P 52, PMPCB



Tested Applications

Positive WB detected inMCF7 cells, HepG2 cells, human liver tissue, MCF-7 cells, rat liver tissue
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

16064-1-AP targets PMPCB in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PMPCB fusion protein Ag8937
Full Name peptidase (mitochondrial processing) beta
Calculated molecular weight 489 aa, 54 kDa
Observed molecular weight 43-52 kDa
GenBank accession numberBC010398
Gene symbol PMPCB
Gene ID (NCBI) 9512
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PMPCB(Mitochondrial-processing peptidase subunit beta) is also named as MPPB, P-52 and belongs to the peptidase M16 family. It cleaves presequences (transit peptides) from mitochondrial protein precursors. It is a mitochondrial matrix marker and Tom70, cytochrome c heme lyase (CCHL) are markers for outer membrane, intermembrane space(PMID:12574118).

Protocols

Product Specific Protocols
WB protocol for PMPCB antibody 16064-1-APDownload protocol
IHC protocol for PMPCB antibody 16064-1-APDownload protocol
IF protocol for PMPCB antibody 16064-1-APDownload protocol
IP protocol for PMPCB antibody 16064-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Hum Mol Genet

LRRK2 impairs PINK1/Parkin-dependent mitophagy via its kinase activity: pathologic insights into Parkinson's disease.

Authors - Fiona Bonello

Mol Cell Biol

LONP1 Is Required for Maturation of a Subset of Mitochondrial Proteins, and Its Loss Elicits an Integrated Stress Response.

Authors - Olga Zurita Rendón

J Mol Cell Cardiol

Embryonic cardiomyocytes can orchestrate various cell protective mechanisms to survive mitochondrial stress.

Authors - Manuela Magarin
humanWB

Sci Rep

AKT signalling selectively regulates PINK1 mitophagy in SHSY5Y cells and human iPSC-derived neurons.

Authors - Marc P M Soutar
humanWB

Hum Mol Genet

The endoplasmic reticulum-mitochondria interface is perturbed in PARK2 knockout mice and patients with PARK2 mutations.

Authors - Clément A Gautier
humanWB

EMBO Rep

Mitochondrial processing peptidase regulates PINK1 processing, import and Parkin recruitment.

Authors - Greene Andrew W AW