PMPCB Polyclonal antibody

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PMPCB Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 16064-1-AP Copied!

Publications (29)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

Beta MPP, MPP11, MPPB, MPPP52, P 52, PMPCB

• 

  WB analysis of MCF-7 using 16064-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 16064-1-AP (PMPCB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human liver using 16064-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 16064-1-AP (PMPCB antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of MCF-7 using 16064-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 16064-1-AP (PMPCB Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of rat liver using 16064-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 16064-1-AP (PMPCB Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HepG2 using 16064-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16064-1-AP (PMPCB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of MCF-7 using 16064-1-AP

  IP result of anti-PMPCB (IP:16064-1-AP, 3ug; Detection:16064-1-AP 1:500) with MCF-7 cells lysate 2560ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human lung cancer using 16064-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16064-1-AP (PMPCB antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HepG2 using 16064-1-AP

  Immunofluorescent analysis of HepG2 cells using 16064-1-AP (PMPCB antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HepG2 using 16064-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 16064-1-AP (PMPCB antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: MCF7 cells, HepG2 cells, human liver tissue, MCF-7 cells, rat liver tissue
• Positive IP detected in: MCF-7 cells
• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:300-1:1200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16064-1-AP targets PMPCB in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rabbit
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag8937

  Product name: Recombinant human PMPCB protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-349 aa of BC010398

  Sequence: MAAAAARVVLSSAARRRLWGFSESLLIRGAAGRSLYFGENRLRSTQAATQVVLNVPETRVTCLESGLRVASEDSGLSTCTVGLWIDAGSRYENEKNNGTAHFLEHMAFKGTKKRSQLDLELEIENMGAHLNAYTSREQTVYYAKAFSKDLPRAVEILADIIQNSTLGEAEIERERGVILREMQEVETNLQEVVFDYLHATAYQNTALGRTILGPTENIKSISRKDLVDYITTHYKGPRIVLAAAGGVSHDELLDLAKFHFGDSLCTHKGEIPALPPCKFTGSEIRVRDDKMPLAHLAIAVEAVGWAHPDTICLMVANTLIGNWDRSFGGGMNLSSKLAQLTCHGNLCHS

• Full Name: peptidase (mitochondrial processing) beta
• Calculated Molecular Weight: 489 aa, 54 kDa
• Observed Molecular Weight: 43-52 kDa
• GenBank Accession Number: BC010398
• Gene Symbol: PMPCB
• Gene ID (NCBI): 9512
• RRID: AB_2167122
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O75439
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PMPCB(Mitochondrial-processing peptidase subunit beta) is also named as MPPB, P-52 and belongs to the peptidase M16 family. It cleaves presequences (transit peptides) from mitochondrial protein precursors. It is a mitochondrial matrix marker and Tom70, cytochrome c heme lyase (CCHL) are markers for outer membrane, intermembrane space(PMID:12574118).

Publications

What published studies show

PMPCB (Mitochondrial-processing peptidase subunit beta) polyclonal antibody (Cat# 16064-1-AP) has accumulated 29 citations across journals including Nature, Nat Cell Biol, Nat Commun, Autophagy, Mol Cell, EMBO J, Cell Reports, Sci Adv, and Elife. The associated research spans mitochondrial biology, mitophagy, protein import and processing, neurodegeneration (Parkinson's disease), apoptosis, cancer cell fitness, cardiomyopathy, sepsis-associated acute kidney injury, and Fragile X syndrome.

Automatically generated from the 29 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Nature; Mitochondrial stress is relayed to the cytosol by an OMA1-DELE1-HRI pathway.; Authors - Xiaoyan Guo
human	WB	Nat Chem; Small-molecule binding-site discovery using silyl ether-enabled chemoproteomics.; Authors - Chau Ngo
human	WB	Nat Cell Biol; PARL mediates Smac proteolytic maturation in mitochondria to promote apoptosis.; Authors - Shotaro Saita
	WB,IF	Autophagy; The TOMM machinery is a molecular switch in PINK1 and PARK2/PARKIN-dependent mitochondrial clearance.; Authors - Giulia Bertolin ; KD Validated
human	WB	Nat Commun; DELE1 tracks perturbed protein import and processing in human mitochondria.; Authors - Evelyn Fessler
human	WB	Mol Cell; A mitochondrial iron-responsive pathway regulated by DELE1; Authors - Yusuke Sekine