|Positive WB detected in||MCF7 cells, HepG2 cells, human liver tissue, MCF-7 cells, rat liver tissue|
|Positive IP detected in||MCF-7 cells|
|Positive IHC detected in||human lung cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:3000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:300-1:1200|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
16064-1-AP targets PMPCB in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||PMPCB fusion protein Ag8937|
|Full Name||peptidase (mitochondrial processing) beta|
|Calculated molecular weight||489 aa, 54 kDa|
|Observed molecular weight||43-52 kDa|
|GenBank accession number||BC010398|
|Gene ID (NCBI)||9512|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
PMPCB(Mitochondrial-processing peptidase subunit beta) is also named as MPPB, P-52 and belongs to the peptidase M16 family. It cleaves presequences (transit peptides) from mitochondrial protein precursors. It is a mitochondrial matrix marker and Tom70, cytochrome c heme lyase (CCHL) are markers for outer membrane, intermembrane space(PMID:12574118).
|Product Specific Protocols|
|WB protocol for PMPCB antibody 16064-1-AP||Download protocol|
|IHC protocol for PMPCB antibody 16064-1-AP||Download protocol|
|IF protocol for PMPCB antibody 16064-1-AP||Download protocol|
|IP protocol for PMPCB antibody 16064-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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