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  • KD/KO Validated

PMPCB Polyclonal antibody

PMPCB Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 16064-1-AP

Synonyms

Beta MPP, MPP11, MPPB, MPPP52, P 52, PMPCB



Tested Applications

Positive WB detected inMCF7 cells, HepG2 cells, human liver tissue, MCF-7 cells, rat liver tissue
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:300-1:1200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16064-1-AP targets PMPCB in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PMPCB fusion protein Ag8937
Full Name peptidase (mitochondrial processing) beta
Calculated Molecular Weight 489 aa, 54 kDa
Observed Molecular Weight 43-52 kDa
GenBank Accession NumberBC010398
Gene Symbol PMPCB
Gene ID (NCBI) 9512
RRIDAB_2167122
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PMPCB(Mitochondrial-processing peptidase subunit beta) is also named as MPPB, P-52 and belongs to the peptidase M16 family. It cleaves presequences (transit peptides) from mitochondrial protein precursors. It is a mitochondrial matrix marker and Tom70, cytochrome c heme lyase (CCHL) are markers for outer membrane, intermembrane space(PMID:12574118).

Protocols

Product Specific Protocols
WB protocol for PMPCB antibody 16064-1-APDownload protocol
IHC protocol for PMPCB antibody 16064-1-APDownload protocol
IF protocol for PMPCB antibody 16064-1-APDownload protocol
IP protocol for PMPCB antibody 16064-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nature

Mitochondrial stress is relayed to the cytosol by an OMA1-DELE1-HRI pathway.

Authors - Xiaoyan Guo
humanWB

Nat Cell Biol

PARL mediates Smac proteolytic maturation in mitochondria to promote apoptosis.

Authors - Shotaro Saita
humanWB

Nat Commun

DELE1 tracks perturbed protein import and processing in human mitochondria.

Authors - Evelyn Fessler
humanWB

EMBO J

PARL partitions the lipid transfer protein STARD7 between the cytosol and mitochondria.

Authors - Shotaro Saita
WB,IF

Autophagy

The TOMM machinery is a molecular switch in PINK1 and PARK2/PARKIN-dependent mitochondrial clearance.

Authors - Giulia Bertolin
  • KD Validated
humanWB

Cell Rep

Sam50 Regulates PINK1-Parkin-Mediated Mitophagy by Controlling PINK1 Stability and Mitochondrial Morphology.

Authors - Fenglei Jian