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  • KD/KO Validated

POLD1 Polyclonal antibody

POLD1 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC,ELISA

Conjugate

Unconjugated

Cat no : 15646-1-AP

Synonyms

CDC2, POLD, POLD1



Tested Applications

Positive WB detected inJurkat cells, MCF-7 cells, K-562 cells, HeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman breast cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

15646-1-AP targets POLD1 in WB, IP, IHC,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen POLD1 fusion protein Ag8178
Full Name polymerase (DNA directed), delta 1, catalytic subunit 125kDa
Calculated molecular weight 1107 aa, 124 kDa
Observed molecular weight 124 kDa
GenBank accession numberBC008800
Gene symbol POLD1
Gene ID (NCBI) 5424
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

POLD1, also known as DNA polymerase subunit delta p125, is 1107 amino acid protein, which belongs to the DNA polymerase type-B family. POLD1 colocalizes with PCNA and POLD3 at S phase replication sites (PubMed:11595739). As the catalytic component of the trimeric (Pol-delta3 complex) and tetrameric DNA polymerase delta complexes (Pol-delta4 complex), plays a crucial role in high fidelity genome replication, including in lagging strand synthesis, and repair. A previous study demonstrated that overexpression of POLD1 is associated with an increased risk of breast cancer. (PMID: 30344713 )

Protocols

Product Specific Protocols
WB protocol for POLD1 antibody 15646-1-APDownload protocol
IHC protocol for POLD1 antibody 15646-1-APDownload protocol
IP protocol for POLD1 antibody 15646-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Haematologica

Dimeric ferrochelatase bridges ABCB7 and ABCB10 homodimers in an architecturally defined molecular complex required for heme biosynthesis.

Authors - Nunziata Maio
humanWB

Nat Microbiol

Core components of DNA lagging strand synthesis machinery are essential for hepatitis B virus cccDNA formation.

Authors - Lei Wei
humanWB

Oncol Lett

Elevated expression of POLD1 is associated with poor prognosis in breast cancer.

Authors - Qinghong Qin
  • KD Validated
humanWB

Onco Targets Ther

p53 inhibits the expression of p125 and the methylation of POLD1 gene promoter by downregulating the Sp1-induced DNMT1 activities in breast cancer.

Authors - Liang Zhang

J Biol Chem

Cellular requirements for iron-sulfur cluster insertion into the antiviral radical SAM protein viperin.

Authors - Arunkumar S Upadhyay
humanWB

Hum Mol Genet

ISCA1 mutation in a patient with infantile-onset leukodystrophy causes defects in mitochondrial [4Fe-4S] proteins.

Authors - Alessandra Torraco