Validation Data Gallery
|Positive WB detected in||Jurkat cells, MCF-7 cells, HeLa cells, K-562 cells|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human breast cancer tissue, human colon cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:3000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
15646-1-AP targets POLD1 in WB, IP, IHC, ELISA applications and shows reactivity with human samples.
|Cited Reactivity||human, mouse, canine|
|Host / Isotype||Rabbit / IgG|
|Immunogen||POLD1 fusion protein Ag8178|
|Full Name||polymerase (DNA directed), delta 1, catalytic subunit 125kDa|
|Calculated molecular weight||1107 aa, 124 kDa|
|Observed molecular weight||124 kDa|
|GenBank accession number||BC008800|
|Gene ID (NCBI)||5424|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
POLD1, also known as DNA polymerase subunit delta p125, is 1107 amino acid protein, which belongs to the DNA polymerase type-B family. POLD1 colocalizes with PCNA and POLD3 at S phase replication sites (PubMed:11595739). As the catalytic component of the trimeric (Pol-delta3 complex) and tetrameric DNA polymerase delta complexes (Pol-delta4 complex), plays a crucial role in high fidelity genome replication, including in lagging strand synthesis, and repair. A previous study demonstrated that overexpression of POLD1 is associated with an increased risk of breast cancer. (PMID: 30344713 )
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