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  • KD/KO Validated

OCT1 Polyclonal antibody

OCT1 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


human and More (3)





Cat no : 10387-1-AP


NF A1, Oct 1, OCT1, Octamer binding protein 1, OTF 1, OTF1, POU class 2 homeobox 1, POU2F1

Tested Applications

Positive WB detected inHEK-293 cells, NCCIT cells, HeLa cells, HepG2 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman colon cancer tissue, human lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10387-1-AP targets OCT1 in WB, IP, IF, IHC, ChIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, rat, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen OCT1 fusion protein Ag0586
Full Name POU class 2 homeobox 1
Calculated Molecular Weight 76 kDa
Observed Molecular Weight 90 kDa
GenBank Accession NumberBC003571
Gene Symbol POU2F1
Gene ID (NCBI) 5451
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The POU class 2 homeobox 1 (POU2F1), also known as octamer-binding transcription factor-1 (OCT-1), is a ubiquitous nuclear protein that regulates the transcription of target genes by binding to a cis-acting octamer element(5'-ATTTGCAT-3'). It participated in the regulation of housekeeping genes, including histone H2B and snRNAs, for cell differentiation and proliferation [PMID:3462701]. In addition, it functions as a cellular stress sensor that modulates activities of tissue-specific target genes involved in immune and inflammatory responses [PMID:16322220]. It also acts as a repressor for the cytokine-inducible, NF-kB-dependent expression of E-selectin and the vascular cell adhesion molecule during vascular inflammation. Otherwise, it can interact with nuclear hormone receptors, such as the retinoid X receptor, the thyroid hormone receptor and the glucocorticoid receptor, and influence their transcription activity[PMID:17192276].


Product Specific Protocols
WB protocol for OCT1 antibody 10387-1-APDownload protocol
IHC protocol for OCT1 antibody 10387-1-APDownload protocol
IF protocol for OCT1 antibody 10387-1-APDownload protocol
IP protocol for OCT1 antibody 10387-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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