OCT1 Polyclonal antibody

OCT1 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, pig and More (2)

Applications

WB, IP, IHC, IF, ChIP,ELISA

Conjugate

Unconjugated

Cat no : 10387-1-AP

Synonyms

NF A1, Oct 1, OCT1, Octamer binding protein 1, OTF 1, OTF1, POU class 2 homeobox 1, POU2F1



Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, NCCIT cell
Positive IP detected inHeLa cells
Positive IHC detected inhuman colon cancer tissue, human lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

10387-1-AP targets OCT1 in WB, IP, IHC, IF, ChIP,ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Cited Reactivity human, mouse, pig, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen OCT1 fusion protein Ag0586
Full Name POU class 2 homeobox 1
Calculated molecular weight 76 kDa
Observed molecular weight 90 kDa
GenBank accession numberBC003571
Gene symbol POU2F1
Gene ID (NCBI) 5451
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The POU class 2 homeobox 1 (POU2F1), also known as octamer-binding transcription factor-1 (OCT-1), is a ubiquitous nuclear protein that regulates the transcription of target genes by binding to a cis-acting octamer element(5’-ATTTGCAT-3’). It participated in the regulation of housekeeping genes, including histone H2B and snRNAs, for cell differentiation and proliferation [PMID:3462701]. In addition, it functions as a cellular stress sensor that modulates activities of tissue-specific target genes involved in immune and inflammatory responses [PMID:16322220]. It also acts as a repressor for the cytokine-inducible, NF-kB-dependent expression of E-selectin and the vascular cell adhesion molecule during vascular inflammation. Otherwise, it can interact with nuclear hormone receptors, such as the retinoid X receptor, the thyroid hormone receptor and the glucocorticoid receptor, and influence their transcription activity[PMID:17192276].

Protocols

Product Specific Protocols
WB protocol for OCT1 antibody 10387-1-APDownload protocol
IHC protocol for OCT1 antibody 10387-1-APDownload protocol
IF protocol for OCT1 antibody 10387-1-APDownload protocol
IP protocol for OCT1 antibody 10387-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Age (Dordr)

Aging increases the susceptibility of cisplatin-induced nephrotoxicity.

Authors - Jiagen Wen
humanWB

Oncotarget

POU2F1 over-expression correlates with poor prognoses and promotes cell growth and epithelial-to-mesenchymal transition in hepatocellular carcinoma.

Authors - Yonghao Zhong
ratWB

Microbiol Immunol

Susceptibility of rat immortalized neuronal cell line Rn33B expressing equine major histocompatibility class 1 to equine herpesvirus-1 infection is differentiation-dependent.

Authors - Erina Minato
humanWB

Int J Oncol

Oncogenic role of the TP53-induced glycolysis and apoptosis regulator in nasopharyngeal carcinoma through NF-κB pathway modulation.

Authors - Ming Zhao
ratWB

PLoS One

The farnesoid X receptor agonist obeticholic acid upregulates biliary excretion of asymmetric dimethylarginine via MATE-1 during hepatic ischemia/reperfusion injury.

Authors - Andrea Ferrigno
mouseWB

Mol Cell Endocrinol

MiR-451a attenuates free fatty acids-mediated hepatocyte steatosis by targeting the thyroid hormone responsive spot 14 gene.

Authors - Ni Zeng