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  • KD/KO Validated

OCT1 Polyclonal antibody

OCT1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (3)

Applications

WB, IP, IHC, IF, ChIP, ELISA

Conjugate

Unconjugated

Cat no : 10387-1-AP

Synonyms

NF A1, Oct 1, OCT1, Octamer binding protein 1, OTF 1, OTF1, POU class 2 homeobox 1, POU2F1



Tested Applications

Positive WB detected inHEK-293 cells, NCCIT cells, HeLa cells, HepG2 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman colon cancer tissue, human lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

10387-1-AP targets OCT1 in WB, IP, IHC, IF, ChIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, rat, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen OCT1 fusion protein Ag0586
Full Name POU class 2 homeobox 1
Calculated molecular weight 76 kDa
Observed molecular weight 90 kDa
GenBank accession numberBC003571
Gene symbol POU2F1
Gene ID (NCBI) 5451
RRIDAB_2167052
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The POU class 2 homeobox 1 (POU2F1), also known as octamer-binding transcription factor-1 (OCT-1), is a ubiquitous nuclear protein that regulates the transcription of target genes by binding to a cis-acting octamer element(5'-ATTTGCAT-3'). It participated in the regulation of housekeeping genes, including histone H2B and snRNAs, for cell differentiation and proliferation [PMID:3462701]. In addition, it functions as a cellular stress sensor that modulates activities of tissue-specific target genes involved in immune and inflammatory responses [PMID:16322220]. It also acts as a repressor for the cytokine-inducible, NF-kB-dependent expression of E-selectin and the vascular cell adhesion molecule during vascular inflammation. Otherwise, it can interact with nuclear hormone receptors, such as the retinoid X receptor, the thyroid hormone receptor and the glucocorticoid receptor, and influence their transcription activity[PMID:17192276].

Protocols

Product Specific Protocols
WB protocol for OCT1 antibody 10387-1-APDownload protocol
IHC protocol for OCT1 antibody 10387-1-APDownload protocol
IF protocol for OCT1 antibody 10387-1-APDownload protocol
IP protocol for OCT1 antibody 10387-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
pigChIP

Mol Biol Evol

Long-term artificial selection reveals a role of TCTP in autophagy in mammalian cells.

Authors - Ke Chen
mouseIF

Food Funct

Anti-hyperuricemic potential of stevia (Stevia rebaudiana Bertoni) residue extract in hyperuricemic mice

Authors - Arshad Mehmood
ratWB

Food Funct

Ferulic acid supplementation alleviates hyperuricemia in high-fructose/fat diet-fed rats via promoting uric acid excretion and mediating the gut microbiota

Authors - Nanhai Zhang
humanWB

Cell Oncol (Dordr)

The POU2F1/miR-4490/USP22 axis regulates cell proliferation and metastasis in gastric cancer.

Authors - Yizhi Xiao
humanWB

iScience

MASTL is enriched in cancerous and pluripotent stem cells and influences OCT1/OCT4 levels.

Authors - Elisa Närvä
  • KD Validated
mouseWB

Mol Cell Endocrinol

MiR-451a attenuates free fatty acids-mediated hepatocyte steatosis by targeting the thyroid hormone responsive spot 14 gene.

Authors - Ni Zeng