POU4F2 Polyclonal antibody

POU4F2 Polyclonal Antibody for ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF,ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 55042-1-AP

Synonyms

Brain 3B, Brn 3b, BRN3.2, BRN3B, POU class 4 homeobox 2, POU4F2

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Validation Data Gallery

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  WB analysis of mouse eye using 55042-1-AP

  mouse eye tissue were subjected to SDS PAGE followed by western blot with 55042-1-AP (POU4F2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Tested Applications

Recommended dilution

Application	Dilution
Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 2 publications below
• IF: See 2 publications below

Product Information

55042-1-AP targets POU4F2 in WB, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: POU class 4 homeobox 2
• Calculated molecular weight: 43 kDa
• Observed molecular weight: 40-44 kDa
• GenBank accession number: NM_004575
• Gene symbol: POU4F2
• Gene ID (NCBI): 5458
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

POU4F2,also named as BRN3.2, BRN3B and Brn-3b, belongs to the POU transcription factor family and Class-4 subfamily. It is a transcription factor. POU4F2 may play a role in determining or maintaining the identities of a small subset of visual system neurons. The antibody is specific to POU4F2.

Publications

Species	Application	Title
		Stem Cell Res Ther; Protective effects of human iPS-derived retinal pigmented epithelial cells on retinal degenerative disease.; Authors - Deliang Zhu
mouse	WB,IF	Sci Rep; 661W is a retinal ganglion precursor-like cell line in which glaucoma-associated optineurin mutants induce cell death selectively.; Authors - Zuberwasim Sayyad
human	WB, IF	Neurochem Int; Human primary retinal cells as an in-vitro model for investigating defective signalling caused by OPTN mutants associated with glaucoma.; Authors - Zuberwasim Sayyad