POU4F2 Polyclonal antibody

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  WB analysis of mouse brain using 55042-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55042-1-AP (POU4F2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 55042-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 55042-1-AP (POU4F2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse eye using 55042-1-AP

  mouse eye tissue were subjected to SDS PAGE followed by western blot with 55042-1-AP (POU4F2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

POU4F2 Polyclonal Antibody for WB, ELISA

Cat No. 55042-1-AP

Publications(4)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, ELISA

Synonyms

Brn-3B, BRN3B, Brain-specific homeobox/POU domain protein 3B, Brain-3B

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Tested Applications

• Positive WB detected in: L02 cells, mouse eye tissue, mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IF: See 2 publications below

Product Information

55042-1-AP targets POU4F2 in WB, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: POU class 4 homeobox 2
• Calculated Molecular Weight: 43 kDa
• Observed Molecular Weight: 40-44 kDa
• GenBank Accession Number: NM_004575
• Gene Symbol: POU4F2
• Gene ID (NCBI): 5458
• RRID: AB_10888633
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q12837
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

POU4F2,also named as BRN3.2, BRN3B and Brn-3b, belongs to the POU transcription factor family and Class-4 subfamily. It is a transcription factor. POU4F2 may play a role in determining or maintaining the identities of a small subset of visual system neurons. The antibody is specific to POU4F2.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
		Stem Cell Res Ther; Protective effects of human iPS-derived retinal pigmented epithelial cells on retinal degenerative disease.; Authors - Deliang Zhu
mouse	WB,IF	Sci Rep; 661W is a retinal ganglion precursor-like cell line in which glaucoma-associated optineurin mutants induce cell death selectively.; Authors - Zuberwasim Sayyad
human	WB, IF	Neurochem Int; Human primary retinal cells as an in-vitro model for investigating defective signalling caused by OPTN mutants associated with glaucoma.; Authors - Zuberwasim Sayyad
human	WB	Life Sci; Intervention of epithelial mesenchymal transition against colon cancer cell growth and metastasis based on SOX21/POU4F2/Hedgehog signaling axis; Authors - Qiaochang Cao

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Alessandro (Verified Customer) (12-09-2023)

good Ab for retinal ganglio cells

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:300
• Cell Tissue Type: Retinal organoids