|Positive WB detected in||HepG2 cells, HeLa cells|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Sample-dependent, check data in validation data gallery|
66826-1-Ig targets PPARA in WB,ELISA applications and shows reactivity with Human, rat samples.
|Tested Reactivity||Human, rat|
|Host / Isotype||Mouse / IgG1|
|Immunogen||PPARA fusion protein Ag7896|
|Full Name||peroxisome proliferator-activated receptor alpha|
|Calculated molecular weight||52 kDa|
|Observed molecular weight||53 kDa|
|GenBank accession number||BC000052|
|Gene ID (NCBI)||5465|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Peroxisome proliferator-activated receptor alpha (PPARA) is a ligand-activated transcription factor that belongs to the PPAR nuclear receptor superfamily. PPARA is essential in the modulation of lipid transport and metabolism, mainly through activating mitochondrial and peroxisomal fatty acid β-oxidation pathways. In addition, PPARA seems to decrease inflammation mainly through direct interaction with NF-κB, causing inhibition of its signaling pathway or reducing the activated levels of NF-κB and subsequent inflammation. Furthermore, PPARA was implicated in the attenuation of oxidative stress in alcoholic liver disease when treated with polyenephosphatidylcholine through downregulation of ROS-generating enzymes such as ethanol-inducible cytochrome P450 2E1 (CYP2E1), acyl-CoA oxidase, and NADPH oxidase. PPARA exists two isoforms and molecular weight of PPARA isoforms are 52 kDa and 22 kDa. The ability of a retinoid X receptor (RXR) to heterodimerize with many nuclear receptors, including LXR, PPAR, NGF1B and RAR, underscores its pivotal role within the nuclear receptor superfamily. Among these heterodimers, PPAR:RXR is considered an important signalling mediator of both PPAR ligands, such as fatty acids, and 9-cis retinoic acid (9-cis RA), an RXR ligand. (PMID: 15103326 ). PPARA can form Heterodimer with RXRA and molecular weight of Heterodimer is about 110 kDa.
Hum Exp Toxicol
Effect of fenofibrate on proliferation of SMMC-7721 cells via regulating cell cycle.
Food Chem Toxicol
Effects of dibutyl phthalate on lipid metabolism in liver and hepatocytes based on PPARα/SREBP-1c/FAS/GPAT/AMPK signal pathway.