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PPARD Monoclonal antibody

PPARD Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1B10E1

Cat no : 60193-1-Ig

Synonyms

FAAR, NR1C2, NUC1, NUCI, NUCII, Nuclear hormone receptor 1, PPAR beta, PPAR delta, PPARB, PPARD



Tested Applications

Positive WB detected inHeLa cells, A549 cells, pig adipose tissue, Jurkat cells, K-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 3 publications below

Product Information

The immunogen of 60193-1-Ig is PPARD Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen PPARD fusion protein Ag16770
Full Name peroxisome proliferator-activated receptor delta
Calculated molecular weight 50 kDa
Observed molecular weight 54 kDa
GenBank accession numberBC002715
Gene symbol PPARD
Gene ID (NCBI) 5467
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Peroxisome proliferator-activator receptor delta(PPARD), a ligand-activated transcription factor, belongs to the PPARs family, which get its name for their chemicals that induce proliferation of peroxisomes, organelles that contributes to the oxidation of fatty acids. PPARD is a receptor for peroxisome proliferator and once activated by a ligand, it binds to and activates the promoter elements of target genes, such as acylCoA oxidase gene. But it act as a repressor for the NPC1L1 gene.

Protocols

Product Specific Protocols
WB protocol for PPARD antibody 60193-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nat Commun

A proteomics landscape of circadian clock in mouse liver.

Authors - Yunzhi Wang
mouseWB

Am J Physiol Endocrinol Metab

Zinc finger protein 407 overexpression upregulates PPAR target gene expression and improves glucose homeostasis in mice

Authors - Alyssa Charrier
humanWB

Clin Transl Med

Cullin-associated and neddylation-dissociated 1 regulate reprogramming of lipid metabolism through SKP1-Cullin-1-F-boxFBXO11 -mediated heterogeneous nuclear ribonucleoprotein A2/B1 ubiquitination and promote hepatocellular carcinoma

Authors - Hao Zhang