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- KD/KO Validated
PPARD Monoclonal antibody
PPARD Monoclonal Antibody for WB, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
human, mouse, rat
Applications
WB, ELISA
Conjugate
Unconjugated
CloneNo.
1B10E1
Cat no : 60193-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, A549 cells, pig adipose tissue, Jurkat cells, K-562 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:6000 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 3 publications below |
Product Information
The immunogen of 60193-1-Ig is PPARD Fusion Protein expressed in E. coli.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Immunogen | PPARD fusion protein Ag16770 |
Full Name | peroxisome proliferator-activated receptor delta |
Calculated molecular weight | 50 kDa |
Observed molecular weight | 54 kDa |
GenBank accession number | BC002715 |
Gene symbol | PPARD |
Gene ID (NCBI) | 5467 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Peroxisome proliferator-activator receptor delta(PPARD), a ligand-activated transcription factor, belongs to the PPARs family, which get its name for their chemicals that induce proliferation of peroxisomes, organelles that contributes to the oxidation of fatty acids. PPARD is a receptor for peroxisome proliferator and once activated by a ligand, it binds to and activates the promoter elements of target genes, such as acylCoA oxidase gene. But it act as a repressor for the NPC1L1 gene.
Protocols
Product Specific Protocols | |
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WB protocol for PPARD antibody 60193-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Am J Physiol Endocrinol Metab Zinc finger protein 407 overexpression upregulates PPAR target gene expression and improves glucose homeostasis in mice | ||
Clin Transl Med Cullin-associated and neddylation-dissociated 1 regulate reprogramming of lipid metabolism through SKP1-Cullin-1-F-boxFBXO11 -mediated heterogeneous nuclear ribonucleoprotein A2/B1 ubiquitination and promote hepatocellular carcinoma |