GADD34 Antibody 31 Publications

Rabbit Polyclonal| Catalog number: 10449-1-AP

Featured Product KD/KO validated

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Freight/Packing

Con: 39 μg/150 μl

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Species specificity:
human, mouse, rat, hamster

Positive WB detected in:
K-562 cells,Jurkat cells, PC-3 cells, HeLa cells, HepG2 cells

Positive IHC detected in:
human pancreas cancer tissue, human colon cancer tissue

Positive IF detected in:
PC-3 cells

Recommended dilution:
WB : 1:300-1:1000
IHC : 1:20-1:200
IF : 1:20-1:200

Product Information


Source:
Rabbit

Purification method:
Antigen affinity purification

Isotype:
IgG

Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Full name:
protein phosphatase 1, regulatory (inhibitor) subunit 15A

Calculated molecular weight:
73 kDa

Observed molecular weight:
100 kDa

GenBank accession number:

Gene ID (NCBI):

Gene symbol
PPP1R15A

Synonyms
GADD34, growth arrest and DNA damage-inducible protein 34, PPP1R15A
Background

GADD34, also named PPP1R15A, belongs to the PPP1R15 family. GADD34 can be triggered as a direct target of activating transcription factor4 (ATF4) under ER stress, it plays a pivotal role in the recovery of cells from shut-down of translation induced by ER stress. It recruits the serine/threonine-protein phosphatase (PP1) to dephosphorylate the translation initiation factor eIF2alpha, thereby reversing the shut-off of protein synthesis initiated by stress-inducible kinases and facilitating recovery of cells from stress. GADD34 down-regulates the TGF-beta signaling pathway by promoting dephosphorylation of TGFB1 via PP1. It may also promote apoptosis by inducing TP53 phosphorylation on 'Ser-15'. Starvation-induced expression of GADD34 reduced mTOR activity and induced autophagy in wild-type mice, but not in GADD34 KO mice. Molecular weight of GADD34 is 100 kDa confirmed in GADD34 KO mice, and Proteintech's GADD34 antibody 10449-1-AP primarily recongize the 100 kDa band.


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