GADD34 Antibody 30 Publications

Rabbit Polyclonal| Catalog number: 10449-1-AP

Featured Product KD/KO validated

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Freight/Packing

Con: 39 μg/150 μl

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Species specificity:
human, mouse, rat, hamster

Positive WB detected in:
K-562 cells, Jurkat cells, PC-3 cells, HeLa cells, HepG2 cells

Positive IHC detected in:
human pancreas cancer tissue, human colon cancer tissue

Positive IF detected in:
PC-3 cells

Recommended dilution:
WB : 1:300-1:1000
IHC : 1:20-1:200
IF : 1:20-1:200

Product Information


Source:
Rabbit

Purification method:
Antigen affinity purification

Isotype:
IgG

Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Full name:
protein phosphatase 1, regulatory (inhibitor) subunit 15A

Calculated molecular weight:
73 kDa

Observed molecular weight:
100 kDa

GenBank accession number:

Gene ID (NCBI):

Gene symbol
PPP1R15A

Synonyms
GADD34, growth arrest and DNA damage-inducible protein 34, PPP1R15A
Background

GADD34, also named PPP1R15A, belongs to the PPP1R15 family. GADD34 can be triggered as a direct target of activating transcription factor4 (ATF4) under ER stress, it plays a pivotal role in the recovery of cells from shut-down of translation induced by ER stress. It recruits the serine/threonine-protein phosphatase (PP1) to dephosphorylate the translation initiation factor eIF2alpha, thereby reversing the shut-off of protein synthesis initiated by stress-inducible kinases and facilitating recovery of cells from stress. GADD34 down-regulates the TGF-beta signaling pathway by promoting dephosphorylation of TGFB1 via PP1. It may also promote apoptosis by inducing TP53 phosphorylation on 'Ser-15'. Starvation-induced expression of GADD34 reduced mTOR activity and induced autophagy in wild-type mice, but not in GADD34 KO mice. Molecular weight of GADD34 appears to be about 100 kDa which was confirmed in GADD34 KO mice and drug treated cells, despite the predicated MW of 73 kDa, and Proteintech's GADD34 antibody 10449-1-AP primarily recongize the 100 kDa band.


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