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  • KD/KO Validated

GADD34 Polyclonal antibody

GADD34 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (5)

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 10449-1-AP

Synonyms

GADD34, growth arrest and DNA damage-inducible protein 34, PPP1R15A



Tested Applications

Positive WB detected inK-562 cells, HeLa cells, Tunicamycin treated HeLa cells, HepG2 cells, Jurkat cells, PC-3 cells
Positive IHC detected inhuman pancreas tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:300-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

10449-1-AP targets GADD34 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity hamster, human, monkey, mouse, pig, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GADD34 fusion protein Ag0578
Full Name protein phosphatase 1, regulatory (inhibitor) subunit 15A
Calculated molecular weight 73 kDa
Observed molecular weight 100 kDa
GenBank accession numberBC003067
Gene symbol PPP1R15A
Gene ID (NCBI) 23645
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GADD34, also named PPP1R15A, belongs to the PPP1R15 family. GADD34 can be triggered as a direct target of activating transcription factor4 (ATF4) under ER stress, it plays a pivotal role in the recovery of cells from shut-down of translation induced by ER stress. It recruits the serine/threonine-protein phosphatase (PP1) to dephosphorylate the translation initiation factor eIF2alpha, thereby reversing the shut-off of protein synthesis initiated by stress-inducible kinases and facilitating recovery of cells from stress. GADD34 down-regulates the TGF-beta signaling pathway by promoting dephosphorylation of TGFB1 via PP1. It may also promote apoptosis by inducing TP53 phosphorylation on 'Ser-15'. Starvation-induced expression of GADD34 reduced mTOR activity and induced autophagy in wild-type mice, but not in GADD34 KO mice. Molecular weight of GADD34 is 100 kDa confirmed in GADD34 KO mice, and Proteintech's GADD34 antibody 10449-1-AP primarily recongize the 100 kDa band.

Protocols

Product Specific Protocols
WB protocol for GADD34 antibody 10449-1-APDownload protocol
IHC protocol for GADD34 antibody 10449-1-APDownload protocol
IF protocol for GADD34 antibody 10449-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Sci Rep

PMID: 30814564

Authors - Vruti Patel
humanWB

Mol Biol Cell

Cytosolic translational responses differ under conditions of severe short-term and long-term mitochondrial stress.

Authors - Lukasz Samluk
humanWB

Cell Cycle

Proscillaridin A slows the prostate cancer progression through triggering the activation of endoplasmic reticulum stress.

Authors - Fan Wang
hamsterWB

Cell Struct Funct

PMID: 23812432

Authors - Ozasa Riwa R
ratWB

J Clin Lab Anal

The endoplasmic reticulum stress induced by tunicamycin affects the viability and autophagy activity of chondrocytes.

Authors - Hao Wu
humanWB

Virology

GADD34 attenuates HIV-1 replication by viral 5'-UTR TAR RNA-mediated translational inhibition.

Authors - Mohammad Ishaq

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Ana (Verified Customer) (03-11-2021)

GADD34 in mouse muscle. 10 ug of protein. BSA blocking. 1:1000 primary ab dilution O/N incubation 4ºC.1:5000 secondary ab HRP conjugated.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Mouse Muscle
GADD34 Antibody Western Blot validation (1:1000 dilution) in Mouse Muscle (Cat no:10449-1-AP)
FH

Maria (Verified Customer) (02-10-2021)

GADD34 in human primary fibroblasts. UT and TN treated. PFA 4% 20 min. Blocking: PBST (0.1% Triton) 10% Goat Serum 1h RT. Primary incubation: 16h 4ºC 1:200 in PBST 10% GS. Secondary: 2h RT 1:500 Goat anti-rabbit Alexa fluor 555 in PBST 10% GS.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: Human primary fibroblasts
GADD34 Antibody Immunofluorescence validation (1:200 dilution) in Human primary fibroblasts (Cat no:10449-1-AP)
FH

Daniel (Verified Customer) (10-01-2019)

Cytoplasmic staining of the tubules of wild-type kidneys.

  • Applications: Immunohistochemistry,
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Mouse kidney
GADD34 Antibody Immunohistochemistry, validation (1:100 dilution) in Mouse kidney (Cat no:10449-1-AP)
FH

Stefan (Verified Customer) (07-31-2019)

See publication:Dalton LE, Clarke HJ, Knight J, Lawson MH, Wason J, Lomas DA, Howat WJ, Rintoul RC, Rassl DM, Marciniak SJ. (2013). The endoplasmic reticulum stress marker CHOP predicts survival in malignant mesothelioma. Br J Cancer. 2013 Apr 2;108(6):1340-7. doi: 10.1038/bjc.2013.66. Epub 2013 Feb 14.https://www.nature.com/articles/bjc201366

  • Applications: Western Blot,Immunohistochemistry,
  • Primary Antibody Dilution: WB 1:1000, IHC 1:100
  • Cell Tissue Type: HCT116 human colon carcinoma
GADD34 Antibody Western Blot,Immunohistochemistry, validation (WB 1:1000, IHC 1:100 dilution) in HCT116 human colon carcinoma (Cat no:10449-1-AP)