PR repeat Polyclonal antibody

PR repeat Polyclonal Antibody for ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (3)

Applications

WB, IHC, IF, Dot Blot,ELISA

Conjugate

Unconjugated

Publications

15

Cat no : 23979-1-AP

Synonyms

C9RAN, GGGCC, poly PR, poly-PR, PR repeat, PRPRPRPRP

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Validation Data Gallery

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  ELISA experiment of peptide using 23979-1-AP

  PR repeat Antibody (23979-1-AP) tested in ELISA. PR repeat peptide was coated onto the microplates at 1.5 µg/well and then incubated with dilution series of PR repeat antibody (23979-1-AP). Bound antibodies were detected with HRP conjugated anti-Rabbit IgG followed by incubation with HRP Substrate and then measuring the resulting absorbance at 450 nm.

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Tested Applications

Recommended dilution

Application	Dilution
Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 8 publications below
• IHC: See 4 publications below
• IF: See 8 publications below

Product Information

23979-1-AP targets PR repeat in WB, IHC, IF, Dot Blot,ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: Drosophila, human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: PR repeat
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

The C9orf72 "GGGGCC" repeat sequence codes five repeat peptide "GA repeat; GAGAGAGAGA", "GP repeat; GPGPGPGPG", "GR repeat; GRGRGRGRG", "AP repeat; APAPAPAPA" and "PR repeat; PRPRPRPRP". It was described previously that aggregated forms of poly-GA and poly-GP proteins do not enter the separation gel (PMID: 26374446).This antibody is used to detect the "PR repeat" sequence. This antibody detects the PRPRPRPRP peptide with dilution 1:4,500 in Elisa.

Publications

Species	Application	Title
human	WB, IHC	Neuropathol Appl Neurobiol; Neurodegeneration in Frontotemporal Lobar Degeneration and Motor Neurone Disease associated with expansions in C9orf72 is linked to TDP-43 pathology and not associated with aggregated forms of dipeptide repeat proteins.; Authors - Yvonne Davidson
human	IF, IHC	JCI Insight; Identification and therapeutic rescue of autophagosome and glutamate receptor defects in C9ORF72 and sporadic ALS neurons.; Authors - Yingxiao Shi
Drosophila	Dot Blot	Cell Rep; Altered MICOS Morphology and Mitochondrial Ion Homeostasis Contribute to Poly(GR) Toxicity Associated with C9-ALS/FTD.; Authors - Shuangxi Li
Drosophila	IF,WB	Mol Cells; C9orf72-Associated Arginine-Rich Dipeptide Repeat Proteins Reduce the Number of Golgi Outposts and Dendritic Branches in Drosophila Neurons.; Authors - Jeong Hyang Park
mouse	IF,WB	Biochim Biophys Acta Mol Cell Res; Expression of C9orf72 hexanucleotide repeat expansion leads to formation of RNA foci and dipeptide repeat proteins but does not influence autophagy or proteasomal function in neuronal cells.; Authors - Stina Leskelä
human	WB	J Cell Sci; SFPQ regulates the accumulation of RNA foci and dipeptide repeat proteins from the expanded repeat mutation in C9orf72.; Authors - Mirjana Malnar

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