PR repeat Polyclonal antibody

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  ELISA experiment of peptide using 23979-1-AP

  PR repeat Antibody (23979-1-AP) tested in ELISA. PR repeat peptide was coated onto the microplates at 1.5 µg/well and then incubated with dilution series of PR repeat antibody (23979-1-AP). Bound antibodies were detected with HRP conjugated anti-Rabbit IgG followed by incubation with HRP Substrate and then measuring the resulting absorbance at 450 nm.

PR repeat Polyclonal Antibody for ELISA

Cat No. 23979-1-AP

Publications(26)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (3)

Applications

WB, IHC, IF, Dot Blot, ELISA

Synonyms

C9RAN, GGGCC, poly PR, poly-PR, PR repeat, PRPRPRPRP

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Tested Applications

• Positive ELISA detected in: peptide

Recommended dilution

• Enzyme-linked Immunosorbent Assay (ELISA): ELISA : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 13 publications below
• IHC: See 5 publications below
• IF: See 15 publications below

Product Information

23979-1-AP targets PR repeat in WB, IHC, IF, Dot Blot, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat, drosophila
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: PR repeat
• RRID: AB_2879388
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The C9orf72 "GGGGCC" repeat sequence codes five repeat peptide "GA repeat; GAGAGAGAGA", "GP repeat; GPGPGPGPG", "GR repeat; GRGRGRGRG", "AP repeat; APAPAPAPA" and "PR repeat; PRPRPRPRP". It was described previously that aggregated forms of poly-GA and poly-GP proteins do not enter the separation gel (PMID: 26374446).This antibody is used to detect the "PR repeat" sequence. This antibody detects the PRPRPRPRP peptide with dilution 1:4,500 in Elisa.

Publications

Species	Application	Title
human	IHC	Nat Neurosci; RPS25 is required for efficient RAN translation of C9orf72 and other neurodegenerative disease-associated nucleotide repeats.; Authors - Shizuka B Yamada
rat	WB, IF	Neuron; Antisense Proline-Arginine RAN Dipeptides Linked to C9ORF72-ALS/FTD Form Toxic Nuclear Aggregates that Initiate In Vitro and In Vivo Neuronal Death.; Authors - Xinmei Wen
mouse	IF	Nat Commun; Motor dysfunction and neurodegeneration in a C9orf72 mouse line expressing poly-PR.; Authors - Zongbing Hao
human	WB, IHC	Acta Neuropathol; Quantitative analysis and clinico-pathological correlations of different dipeptide repeat protein pathologies in C9ORF72 mutation carriers.; Authors - Ian R A Mackenzie
human	WB	EMBO J; NDST3 deacetylates α-tubulin and suppresses V-ATPase assembly and lysosomal acidification.; Authors - Qing Tang
human	WB	EMBO J; The RNA exosome complex degrades expanded hexanucleotide repeat RNA in C9orf72 FTLD/ALS.; Authors - Yuya Kawabe