Tested Applications
| Positive ELISA detected in | peptide |
Recommended dilution
| Application | Dilution |
|---|---|
| Enzyme-linked Immunosorbent Assay (ELISA) | ELISA : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
23979-1-AP targets PR repeat in WB, IHC, IF, Dot Blot, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat, drosophila |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | PR repeat |
| Gene Symbol | |
| Gene ID (NCBI) | |
| RRID | AB_2879388 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The C9orf72 "GGGGCC" repeat sequence codes five repeat peptide "GA repeat; GAGAGAGAGA", "GP repeat; GPGPGPGPG", "GR repeat; GRGRGRGRG", "AP repeat; APAPAPAPA" and "PR repeat; PRPRPRPRP". It was described previously that aggregated forms of poly-GA and poly-GP proteins do not enter the separation gel (PMID: 26374446).This antibody is used to detect the "PR repeat" sequence. This antibody detects the PRPRPRPRP peptide with dilution 1:4,500 in Elisa.
Publications
What published studies show
The PR repeat Polyclonal antibody (Cat# 23979-1-AP) has 28 citations. Citations appear in journals including Cell, Cell Stem Cell, Nature Neuroscience, Molecular Neurodegeneration, Nature Communications, Neuron, Science Translational Medicine, Acta Neuropathologica, EMBO Journal, Cell Reports, JCI Insight, Molecular Neurobiology, Stem Cells, iScience, Frontiers in Cellular Neuroscience, Human Molecular Genetics, Life Science Alliance, Neuropathology and Applied Neurobiology, FEBS Open Biology, and eLife. Research fields center on ALS/FTD, C9ORF72 dipeptide repeat protein toxicity, and neurodegeneration.
| Species | Application | Title |
|---|---|---|
Cell Stem Cell SYF2 suppression mitigates neurodegeneration in models of diverse forms of ALS | ||
Nat Neurosci RPS25 is required for efficient RAN translation of C9orf72 and other neurodegenerative disease-associated nucleotide repeats. | ||
Mol Neurodegener Dipeptide repeat proteins inhibit homology-directed DNA double strand break repair in C9ORF72 ALS/FTD. | ||
Nat Commun Motor dysfunction and neurodegeneration in a C9orf72 mouse line expressing poly-PR. | ||
Neuron Antisense Proline-Arginine RAN Dipeptides Linked to C9ORF72-ALS/FTD Form Toxic Nuclear Aggregates that Initiate In Vitro and In Vivo Neuronal Death. |
